IHC-plus™ GLI2 Antibody (Internal) LS-B75
LifeSpan Biosciences
catalog: LS-B75

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-GLI2 antibody IHC of mouse, testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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GLI2 Antibody (aa600-649) LS-C31484
LifeSpan Biosciences
catalog: LS-C31484

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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IHC-plus™ GLI2 Antibody LS-B2808
LifeSpan Biosciences
catalog: LS-B2808

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-GLI2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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GLI2 Antibody (aa1444-1463) LS-C313075
LifeSpan Biosciences
catalog: LS-C313075

domestic rabbit polyclonal
reactivity: human
application: western blot
citations: 1

WB of GLI2 antibody. Lane 1: MCF-7 Cell Lysate. Lane 2: HELA Cell Lysate. Lane 3: SKOV Cell Lysate. Lane 4: HT1080 Cell Lysate. Lane 5: A549 Cell Lysate.
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GLI2 Antibody (aa416-429) LS-C54915
LifeSpan Biosciences
catalog: LS-C54915

domestic goat polyclonal
reactivity: human
application: immunohistochemistry - paraffin section

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GLI2 Antibody (aa729-1123, clone OTI1F9) LS-C339221
LifeSpan Biosciences
catalog: LS-C339221

mouse monoclonal (OTI1F9)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2.

IHC of paraffin-embedded Human skin tissue using anti-GLI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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GLI2 Antibody (aa729-1123, clone OTI1B2) LS-C339225
LifeSpan Biosciences
catalog: LS-C339225

mouse monoclonal (OTI1B2)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2.
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GLI2 Antibody (aa729-1123, clone OTI1G2) LS-C339241
LifeSpan Biosciences
catalog: LS-C339241

mouse monoclonal (OTI1G2)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2.
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GLI2 Antibody (aa729-1123, clone OTI1G1) LS-C339242
LifeSpan Biosciences
catalog: LS-C339242

mouse monoclonal (OTI1G1)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2.

IHC of paraffin-embedded Human testicular cancer tissue using anti-GLI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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GLI2 Antibody (aa1287-1321) LS-C344252
LifeSpan Biosciences
catalog: LS-C344252

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Fluorescent image of HeLa cells stained with GLI2 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Flow cytometric of HeLa cells with GLI2 Antibody (green) compared to an isotype control of rabbit IgG (blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot of lysates from 293, Jurkat cell line (from left to right) with GLI2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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GLI2 Antibody (aa300-400) LS-C359357
LifeSpan Biosciences
catalog: LS-C359357

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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GLI2 Antibody LS-C408977
LifeSpan Biosciences
catalog: LS-C408977

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

Western blot analysis of extracts of various tissues.
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