IHC-plus™ STK39 / SPAK Antibody LS-B3852
LifeSpan Biosciences
catalog: LS-B3852
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Stk39 in SK-N-SH cell lysate with Stk39 antibody at (A) 1 and (B) 2 ug/ml.

Anti-SPAK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of stk39 in human brain tissue with stk39 antibody at 20 ug/ml.
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STK39 / SPAK Antibody (aa386-415) LS-C98171
LifeSpan Biosciences
catalog: LS-C98171
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

STK39 Antibody western blot of human placenta tissue lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with STK39 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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STK39 / SPAK Antibody (aa346-376) LS-C98726
LifeSpan Biosciences
catalog: LS-C98726
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of lysate from HepG2 cell line, using SPAK Antibody (A363). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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STK39 / SPAK Antibody (aa151-200) LS-C81289
LifeSpan Biosciences
catalog: LS-C81289
domestic rabbit polyclonal
reactivity: human
application: western blot
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STK39 / SPAK Antibody LS-C82871
LifeSpan Biosciences
catalog: LS-C82871
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Stk39 in rat brain tissue lysate with Stk39 antibody at (A) 1 and (B) 2 ug/ml

Immunohistochemistry of Stk39 in human brain tissue with Stk39 antibody at 2.5 µg/mL.
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STK39 / SPAK Antibody (aa291-340) LS-C118325
LifeSpan Biosciences
catalog: LS-C118325
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from COLO cells, using STK39 Antibody. The lane on the right is blocked with the synthesized peptide.
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STK39 / SPAK Antibody (aa277-326) LS-C118326
LifeSpan Biosciences
catalog: LS-C118326
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from COLO cells, using STK39 Antibody. The lane on the right is blocked with the synthesized peptide.
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STK39 / SPAK Antibody (aa351-400) LS-C120422
LifeSpan Biosciences
catalog: LS-C120422
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from COLO205 cells, using STK39 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ STK39 / SPAK Antibody (aa401-414) LS-B8310
LifeSpan Biosciences
catalog: LS-B8310
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

STK39 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-STK39 / SPAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
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STK39 / SPAK Antibody (aa433-460) LS-C158271
LifeSpan Biosciences
catalog: LS-C158271
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Stk39 Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The Stk39 antibody detected the Stk39 protein (arrow).
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STK39 / SPAK Antibody LS-C161567
LifeSpan Biosciences
catalog: LS-C161567
mouse monoclonal
reactivity: human
application: western blot

STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).
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STK39 / SPAK Antibody LS-C161568
LifeSpan Biosciences
catalog: LS-C161568
mouse monoclonal
reactivity: human
application: western blot

STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).
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STK39 / SPAK Antibody (clone OTI2E10) LS-C114596
LifeSpan Biosciences
catalog: LS-C114596
mouse monoclonal (OTI2E10)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-STK39 monoclonal antibody.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
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STK39 / SPAK Antibody (clone OTI3C12) LS-C114601
LifeSpan Biosciences
catalog: LS-C114601
mouse monoclonal (OTI3C12)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK39 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39.

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).

Anti-STK39 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STK39.
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STK39 / SPAK Antibody (phospho-Ser311) LS-C199616
LifeSpan Biosciences
catalog: LS-C199616
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using STK39 (Phospho-Ser311) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COLO205 cells, using STK39 (Phospho-Ser311) Antibody. The lane on the right is blocked with the phospho peptide.
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STK39 / SPAK Antibody (phospho-Ser325) LS-C199617
LifeSpan Biosciences
catalog: LS-C199617
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using STK39 (Phospho-Ser325) Antibody. The picture on the right is blocked with the phospho peptide.
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STK39 / SPAK Antibody (aa271-320) LS-C200733
LifeSpan Biosciences
catalog: LS-C200733
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HeLa cells using STK39 antibody.
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STK39 / SPAK Antibody (aa257-306) LS-C200734
LifeSpan Biosciences
catalog: LS-C200734
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HepG2 cells using STK39 antibody.
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STK39 / SPAK Antibody (aa345-394) LS-C200735
LifeSpan Biosciences
catalog: LS-C200735
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from RAW264.7cells using STK39 antibody.
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STK39 / SPAK Antibody (aa346-376, AP) LS-C270091
LifeSpan Biosciences
catalog: LS-C270091
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa346-376, APC) LS-C270092
LifeSpan Biosciences
catalog: LS-C270092
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa346-376, Biotin) LS-C270093
LifeSpan Biosciences
catalog: LS-C270093
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa346-376, Azide-free, HRP) LS-C270094
LifeSpan Biosciences
catalog: LS-C270094
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa346-376, FITC) LS-C270095
LifeSpan Biosciences
catalog: LS-C270095
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa346-376, PE) LS-C270098
LifeSpan Biosciences
catalog: LS-C270098
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa433-460, AP) LS-C270583
LifeSpan Biosciences
catalog: LS-C270583
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: AP
application: western blot, ELISA
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STK39 / SPAK Antibody (aa433-460, Biotin) LS-C270584
LifeSpan Biosciences
catalog: LS-C270584
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA
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STK39 / SPAK Antibody (aa433-460, FITC) LS-C270585
LifeSpan Biosciences
catalog: LS-C270585
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA
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STK39 / SPAK Antibody (aa433-460, APC) LS-C270586
LifeSpan Biosciences
catalog: LS-C270586
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: APC
application: western blot, ELISA
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STK39 / SPAK Antibody (aa433-460, Azide-free, HRP) LS-C270587
LifeSpan Biosciences
catalog: LS-C270587
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA
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STK39 / SPAK Antibody (aa433-460, PE) LS-C270588
LifeSpan Biosciences
catalog: LS-C270588
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA
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STK39 / SPAK Antibody (aa386-415, APC) LS-C282851
LifeSpan Biosciences
catalog: LS-C282851
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa386-415, AP) LS-C282852
LifeSpan Biosciences
catalog: LS-C282852
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa386-415, FITC) LS-C282853
LifeSpan Biosciences
catalog: LS-C282853
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa386-415, Biotin) LS-C282854
LifeSpan Biosciences
catalog: LS-C282854
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa386-415, Azide-free, HRP) LS-C282856
LifeSpan Biosciences
catalog: LS-C282856
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa386-415, PE) LS-C282859
LifeSpan Biosciences
catalog: LS-C282859
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa425-450) LS-C286893
LifeSpan Biosciences
catalog: LS-C286893
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
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STK39 / SPAK Antibody (aa375-425) LS-C289202
LifeSpan Biosciences
catalog: LS-C289202
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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STK39 / SPAK Antibody (aa425-450) LS-C289203
LifeSpan Biosciences
catalog: LS-C289203
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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STK39 / SPAK Antibody (aa346-376) LS-C325312
LifeSpan Biosciences
catalog: LS-C325312
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (aa433-460) LS-C325459
LifeSpan Biosciences
catalog: LS-C325459
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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STK39 / SPAK Antibody (aa386-415) LS-C328709
LifeSpan Biosciences
catalog: LS-C328709
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (Internal) LS-C368943
LifeSpan Biosciences
catalog: LS-C368943
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of SPAK expression in Jurkat (A); HepG2 (B) whole cell lysates.

Immunohistochemical analysis of SPAK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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STK39 / SPAK Antibody (aa63-545) LS-C373817
LifeSpan Biosciences
catalog: LS-C373817
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of STK39 / SPAK antibody.

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STK39 / SPAK Antibody (250-330 aa) LS-C381399
LifeSpan Biosciences
catalog: LS-C381399
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (260-340 aa) LS-C381648
LifeSpan Biosciences
catalog: LS-C381648
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry
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to the supplier
STK39 / SPAK Antibody (250-330 aa) LS-C386150
LifeSpan Biosciences
catalog: LS-C386150
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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STK39 / SPAK Antibody (260-340 aa) LS-C386151
LifeSpan Biosciences
catalog: LS-C386151
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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STK39 / SPAK Antibody (320-400 aa, Internal) LS-C386152
LifeSpan Biosciences
catalog: LS-C386152
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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