IHC-plus™ GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-A9453
LifeSpan Biosciences
catalog: LS-A9453

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-GJA1 / Connexin 43 antibody IHC of human testis, seminiferous tubules and Leydig cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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GJA1 / CX43 / Connexin 43 Antibody (aa333-346) LS-C101308
LifeSpan Biosciences
catalog: LS-C101308

domestic goat polyclonal
reactivity: human
application: western blot

Antibody (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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GJA1 / CX43 / Connexin 43 Antibody (aa107-138) LS-C97725
LifeSpan Biosciences
catalog: LS-C97725

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of lysates from HeLa, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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IHC-plus™ GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-B9771
LifeSpan Biosciences
catalog: LS-B9771

domestic goat polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Anti-GJA1 / CX43 / Connexin 43 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot. Endogenous CX43 detected with GJA1 / CX43 / Connexin 43 antibody at 1:1000 dilution. Lysate at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Immunofluorescence. Immunostaining of primary RPE cells with CX43 antibody at 1:100 dilution.
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GJA1 / CX43 / Connexin 43 Antibody (aa252-270) LS-C14553
LifeSpan Biosciences
catalog: LS-C14553

mouse monoclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

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GJA1 / CX43 / Connexin 43 Antibody (2nd Cytoplasmic Domain) LS-C14562
LifeSpan Biosciences
catalog: LS-C14562

mouse monoclonal
reactivity: human, mouse
application: western blot, ELISA

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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-C14563
LifeSpan Biosciences
catalog: LS-C14563

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

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GJA1 / CX43 / Connexin 43 Antibody LS-C14565
LifeSpan Biosciences
catalog: LS-C14565

mouse monoclonal
reactivity: human, rat
application: western blot, ELISA, immunocytochemistry

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GJA1 / CX43 / Connexin 43 Antibody (aa91-140) LS-C30040
LifeSpan Biosciences
catalog: LS-C30040

domestic rabbit polyclonal
reactivity: human
application: western blot

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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-C14554
LifeSpan Biosciences
catalog: LS-C14554

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-C14557
LifeSpan Biosciences
catalog: LS-C14557

mouse monoclonal
reactivity: human, rat
application: western blot, ELISA, immunocytochemistry

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GJA1 / CX43 / Connexin 43 Antibody (Ser368) LS-C79740
LifeSpan Biosciences
catalog: LS-C79740

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-C110179
LifeSpan Biosciences
catalog: LS-C110179

domestic rabbit polyclonal
reactivity: human
application: western blot

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GJA1 / CX43 / Connexin 43 Antibody (phospho-Ser367) LS-C117325
LifeSpan Biosciences
catalog: LS-C117325

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Connexin 43 (Phospho-Ser367) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells treated with PMA 200ng/ml 10', using Connexin 43 (Phospho-Ser367) Antibody. The lane on the right is blocked with the phospho peptide.
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GJA1 / CX43 / Connexin 43 Antibody (aa226-275) LS-C117864
LifeSpan Biosciences
catalog: LS-C117864

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using Connexin 43 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Connexin 43 Antibody. The picture on the right is blocked with the synthesized peptide.
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GJA1 / CX43 / Connexin 43 Antibody (aa107-138) LS-C121660
LifeSpan Biosciences
catalog: LS-C121660

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-C123320
LifeSpan Biosciences
catalog: LS-C123320

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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GJA1 / CX43 / Connexin 43 Antibody LS-C137272
LifeSpan Biosciences
catalog: LS-C137272

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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IHC-plus™ GJA1 / CX43 / Connexin 43 Antibody (aa333-382) LS-B7595
LifeSpan Biosciences
catalog: LS-B7595

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-GJA1 / Connexin 43 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of A549 cells, using Connexin 43 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using Connexin 43 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ GJA1 / CX43 / Connexin 43 Antibody (Internal) LS-A9454
LifeSpan Biosciences
catalog: LS-A9454

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-GJA1 / Connexin 43 antibody IHC of human seminiferous tubule and Leydig cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-C171543
LifeSpan Biosciences
catalog: LS-C171543

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus, Magnetic beads) LS-C171544
LifeSpan Biosciences
catalog: LS-C171544

domestic rabbit polyclonal
reactivity: human
conjugate: Bead
application: immunoprecipitation

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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus, Sepharose) LS-C171545
LifeSpan Biosciences
catalog: LS-C171545

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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GJA1 / CX43 / Connexin 43 Antibody (Lys257) LS-C176593
LifeSpan Biosciences
catalog: LS-C176593

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

IHC of Connexin 43 (K257) pAb in paraffin-embedded human brain tissue.

Western blot analysis of Connexin 43 antibody at 1:500 dilution. Lane 1: MCF-7 cell lysate, Lane 2: HeLa cell lysate, Lane 3: Raw264.7 cell lysate.
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GJA1 / CX43 / Connexin 43 Antibody (Arg361) LS-C177684
LifeSpan Biosciences
catalog: LS-C177684

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of Connexin 43 (R361) pAb in extracts from mouse heart cells.

IHC of Connexin 43 (R361) pAb in paraffin-embedded human brain tissue.
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GJA1 / CX43 / Connexin 43 Antibody (phospho-Ser367) LS-C178044
LifeSpan Biosciences
catalog: LS-C178044

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-Connexin 43 (S367) pAb in extracts from Jurkat cells.

IHC of p-Connexin 43 (S367) pAb in paraffin-embedded human brain tissue.
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GJA1 / CX43 / Connexin 43 Antibody (aa366-382) LS-C189648
LifeSpan Biosciences
catalog: LS-C189648

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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GJA1 / CX43 / Connexin 43 Antibody (clone 3E5) LS-C197296
LifeSpan Biosciences
catalog: LS-C197296

mouse monoclonal (3E5)
reactivity: human
application: western blot, ELISA

GJA1 monoclonal antibody (M01), clone 3E5. Western Blot analysis of GJA1 expression in human placenta.
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GJA1 / CX43 / Connexin 43 Antibody (phospho-Ser261) LS-C198823
LifeSpan Biosciences
catalog: LS-C198823

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using Connexin 43 (Phospho-Ser261) Antibody. The picture on the right is blocked with the phospho peptide.
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IHC-plus™ GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-B9770
LifeSpan Biosciences
catalog: LS-B9770

domestic goat polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-GJA1 / CX43 / Connexin 43 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot. Endogenous Cx43 detected with GJA1 / CX43 / Connexin 43 antibody at 1:1000 dilution. Lysate at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Immunofluorescence. Immunostaining of primary RPE cells with CX43 antibody at 1:100 dilution.
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GJA1 / CX43 / Connexin 43 Antibody (aa107-138, AP) LS-C218824
LifeSpan Biosciences
catalog: LS-C218824

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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GJA1 / CX43 / Connexin 43 Antibody (aa107-138, APC) LS-C218825
LifeSpan Biosciences
catalog: LS-C218825

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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GJA1 / CX43 / Connexin 43 Antibody (aa107-138, Biotin) LS-C218826
LifeSpan Biosciences
catalog: LS-C218826

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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GJA1 / CX43 / Connexin 43 Antibody (aa107-138, FITC) LS-C218827
LifeSpan Biosciences
catalog: LS-C218827

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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GJA1 / CX43 / Connexin 43 Antibody (aa107-138, Azide-free, HRP) LS-C218828
LifeSpan Biosciences
catalog: LS-C218828

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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GJA1 / CX43 / Connexin 43 Antibody (aa107-138, PE) LS-C218833
LifeSpan Biosciences
catalog: LS-C218833

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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GJA1 / CX43 / Connexin 43 Antibody (aa180-382) LS-C293522
LifeSpan Biosciences
catalog: LS-C293522

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of recombinant GJA1 / CX43 / Connexin 43. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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GJA1 / CX43 / Connexin 43 Antibody (aa180-382) LS-C293525
LifeSpan Biosciences
catalog: LS-C293525

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Mouse Brain Tissue.

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GJA1 / CX43 / Connexin 43 Antibody (aa180-382) LS-C293528
LifeSpan Biosciences
catalog: LS-C293528

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western Blot; Sample: Mouse Brain Tissue.

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IHC-plus™ GJA1 / CX43 / Connexin 43 Antibody (aa340-390) LS-B11315
LifeSpan Biosciences
catalog: LS-B11315

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-GJA1 / CX43 / Connexin 43 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot analysis of Connexin 43 pAb at a 1:500 dilution. Lane 1: HEK293T cell lysate. Lane 2: Mouse heart tissue lysate. Lane 3: Rat brain tissue lysate.
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GJA1 / CX43 / Connexin 43 Antibody LS-C192784
LifeSpan Biosciences
catalog: LS-C192784

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of HeLa cell lines, using Connexin 43 antibody.
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GJA1 / CX43 / Connexin 43 Antibody LS-C331950
LifeSpan Biosciences
catalog: LS-C331950

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of Mouse heart tissue.
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GJA1 / CX43 / Connexin 43 Antibody LS-C335077
LifeSpan Biosciences
catalog: LS-C335077

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cell lines, using GJA1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
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GJA1 / CX43 / Connexin 43 Antibody (aa351-367) LS-C343860
LifeSpan Biosciences
catalog: LS-C343860

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Connexin 43/GJA1 antibody IHC-frozen: Rat Cardiac Muscle Tissue.

Connexin 43/GJA1 antibody Western blot. WB: Rat Heart Tissue Lysate.
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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-C353999
LifeSpan Biosciences
catalog: LS-C353999

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

Western blot analysis of Connexin 43 expression in HeLa (A); mouse heart (B); rat heart (C) whole cell lysates.
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GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) LS-C356071
LifeSpan Biosciences
catalog: LS-C356071

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of Connexin 43 (pS368) expression in Jurkat (A); A549 (B) whole cell lysates.

Immunohistochemical analysis of Connexin 43 (pS368) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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GJA1 / CX43 / Connexin 43 Antibody (Internal) LS-C358999
LifeSpan Biosciences
catalog: LS-C358999

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of Connexin 43 expression in HeLa (A); MCF7 (B) whole cell lysates.

Immunofluorescent analysis of Connexin 43 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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GJA1 / CX43 / Connexin 43 Antibody (aa233-382) LS-C375818
LifeSpan Biosciences
catalog: LS-C375818

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human tonsil using antibody at 1:100 dilution.
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GJA1 / CX43 / Connexin 43 Antibody (aa233-382) LS-C375819
LifeSpan Biosciences
catalog: LS-C375819

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: Gap junction alpha-1 protein antibody at 6 ug/ml+HeLa whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 43 kDa. Observed band size: 43 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.
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GJA1 / CX43 / Connexin 43 Antibody (300-380 aa) LS-C380672
LifeSpan Biosciences
catalog: LS-C380672

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Connexin 43 (S368) antibody
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GJA1 / CX43 / Connexin 43 Antibody (phospho-Tyr265) LS-C381054
LifeSpan Biosciences
catalog: LS-C381054

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

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GJA1 / CX43 / Connexin 43 Antibody (200-280 aa) LS-C381572
LifeSpan Biosciences
catalog: LS-C381572

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

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GJA1 / CX43 / Connexin 43 Antibody (200-280 aa) LS-C382851
LifeSpan Biosciences
catalog: LS-C382851

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry

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GJA1 / CX43 / Connexin 43 Antibody (310-390 aa, C-terminal) LS-C382857
LifeSpan Biosciences
catalog: LS-C382857

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Connexin 43 antibody
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GJA1 / CX43 / Connexin 43 Antibody (300-380 aa) LS-C382858
LifeSpan Biosciences
catalog: LS-C382858

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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