PHGDH Antibody (aa362-411) LS-C81937
LifeSpan Biosciences
catalog: LS-C81937
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ PHGDH Antibody (clone 4A3-1D6) LS-B5457
LifeSpan Biosciences
catalog: LS-B5457
mouse monoclonal (4A3-1D6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 1

Western blot of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody, clone 4A3-1D6.

Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1 ~10 ug/ml].

Immunoprecipitation of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead.
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PHGDH Antibody (aa249-277) LS-C163573
LifeSpan Biosciences
catalog: LS-C163573
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

PHGDH Antibody western blot of Ramos cell line lysates (15 ug/lane). The PHGDH antibody detected the PHGDH protein (arrow).

PHGDH Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

PHGDH Antibody flow cytometry of Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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PHGDH Antibody (aa140-168) LS-C163574
LifeSpan Biosciences
catalog: LS-C163574
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of PHGDH (arrow) using rabbit polyclonal PHGDH Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (Lane 2).

Formalin-fixed and paraffin-embedded human brain tissue reacted with PHGDH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of HeLa cells using PHGDH Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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PHGDH Antibody LS-C211551
LifeSpan Biosciences
catalog: LS-C211551
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of D-3-phosphoglycerate dehydrogenase antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 59 kDa. Observed band size: 70 kDa Additional bands at: 100 kDa. 36 kDa. We are unsure as to the identity of these extra bands. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PHGDH Antibody (HRP) LS-C211552
LifeSpan Biosciences
catalog: LS-C211552
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Western blot of D-3-phosphoglycerate dehydrogenase antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 59 kDa. Observed band size: 70 kDa Additional bands at: 100 kDa. 36 kDa. We are unsure as to the identity of these extra bands. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PHGDH Antibody (FITC) LS-C211553
LifeSpan Biosciences
catalog: LS-C211553
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

Western blot of D-3-phosphoglycerate dehydrogenase antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 59 kDa. Observed band size: 70 kDa Additional bands at: 100 kDa. 36 kDa. We are unsure as to the identity of these extra bands. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PHGDH Antibody (Biotin) LS-C211554
LifeSpan Biosciences
catalog: LS-C211554
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Western blot of D-3-phosphoglycerate dehydrogenase antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 59 kDa. Observed band size: 70 kDa Additional bands at: 100 kDa. 36 kDa. We are unsure as to the identity of these extra bands. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PHGDH Antibody (FITC) LS-C211739
LifeSpan Biosciences
catalog: LS-C211739
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

Western blot of D-3-phosphoglycerate dehydrogenase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PHGDH Antibody (HRP) LS-C211740
LifeSpan Biosciences
catalog: LS-C211740
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Western blot of D-3-phosphoglycerate dehydrogenase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PHGDH Antibody LS-C211741
LifeSpan Biosciences
catalog: LS-C211741
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of D-3-phosphoglycerate dehydrogenase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PHGDH Antibody (Biotin) LS-C211744
LifeSpan Biosciences
catalog: LS-C211744
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Western blot of D-3-phosphoglycerate dehydrogenase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PHGDH Antibody (aa249-277, AP) LS-C266182
LifeSpan Biosciences
catalog: LS-C266182
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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PHGDH Antibody (aa249-277, APC) LS-C266183
LifeSpan Biosciences
catalog: LS-C266183
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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PHGDH Antibody (aa249-277, PE) LS-C266184
LifeSpan Biosciences
catalog: LS-C266184
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, flow cytometry
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IHC-plus™ PHGDH Antibody (Internal) LS-B10757
LifeSpan Biosciences
catalog: LS-B10757
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate) A: A431 B: Raji 7.5% SDS PAGE PHGDH antibody diluted at 1:10000

Anti-PHGDH / PGDH antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
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PHGDH Antibody (aa249-277) LS-C329967
LifeSpan Biosciences
catalog: LS-C329967
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
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PHGDH Antibody (clone OTI5C4) LS-C340072
LifeSpan Biosciences
catalog: LS-C340072
mouse monoclonal (OTI5C4)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH.

IHC of paraffin-embedded Human liver tissue using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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PHGDH Antibody (clone OTI4A1) LS-C340080
LifeSpan Biosciences
catalog: LS-C340080
mouse monoclonal (OTI4A1)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH.
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PHGDH Antibody (clone OTI9C2) LS-C340121
LifeSpan Biosciences
catalog: LS-C340121
mouse monoclonal (OTI9C2)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH.

IHC of paraffin-embedded Human liver tissue using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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IHC-plus™ PHGDH Antibody (aa254-303) LS-B12727
LifeSpan Biosciences
catalog: LS-B12727
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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