SIRT1 / Sirtuin 1 Antibody (aa207-256) LS-C31402
LifeSpan Biosciences
catalog: LS-C31402
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ SIRT1 / Sirtuin 1 Antibody (clone 7B7) LS-B4520
LifeSpan Biosciences
catalog: LS-B4520
mouse monoclonal (7B7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

SIRT1 monoclonal antibody, clone 7B7 Western blot of SIRT1 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to SIRT1 on HeLa cell (antibody concentration 10 ug/ml).

Detection limit for recombinant GST tagged SIRT1 is approximately 0.1 ng/ml as a capture antibody.
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IHC-plus™ SIRT1 / Sirtuin 1 Antibody (aa577-590) LS-B8356
LifeSpan Biosciences
catalog: LS-B8356
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

SIRT1 antibody (0.05 ug/ml) staining of Jurkat lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-SIRT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
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IHC-plus™ SIRT1 / Sirtuin 1 Antibody (aa207-256) LS-B1564
LifeSpan Biosciences
catalog: LS-B1564
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
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SIRT1 / Sirtuin 1 Antibody (aa551-599) LS-C10179
LifeSpan Biosciences
catalog: LS-C10179
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation
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IHC-plus™ SIRT1 / Sirtuin 1 Antibody (C-Terminus) LS-B1895
LifeSpan Biosciences
catalog: LS-B1895
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-SIRT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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SIRT1 / Sirtuin 1 Antibody LS-C115840
LifeSpan Biosciences
catalog: LS-C115840
chicken polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Immunofluorescence of SIRT1 in rat lung tissue with SIRT1 antibody at 20 ug/ml.

Western blot analysis of SIRT1 in mouse liver tissue lysate with SIRT1 antibody at 1 ug/ml.
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SIRT1 / Sirtuin 1 Antibody (C-Terminus) LS-C137090
LifeSpan Biosciences
catalog: LS-C137090
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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SIRT1 / Sirtuin 1 Antibody (clone 1F3) LS-C169237
LifeSpan Biosciences
catalog: LS-C169237
mouse monoclonal (1F3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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SIRT1 / Sirtuin 1 Antibody (aa41-90) LS-C200670
LifeSpan Biosciences
catalog: LS-C200670
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of the lysates from COLO205 cells using SirT1 antibody.
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SIRT1 / Sirtuin 1 Antibody (aa25-75) LS-C287559
LifeSpan Biosciences
catalog: LS-C287559
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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SIRT1 / Sirtuin 1 Antibody (aa700-C-term) LS-C287560
LifeSpan Biosciences
catalog: LS-C287560
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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SIRT1 / Sirtuin 1 Antibody (aa244-498, clone C4) LS-C292515
LifeSpan Biosciences
catalog: LS-C292515
mouse monoclonal (C4)
reactivity: human
application: western blot

Western Blot; Sample: Recombinant protein.
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SIRT1 / Sirtuin 1 Antibody (aa244-498, Biotin) LS-C298038
LifeSpan Biosciences
catalog: LS-C298038
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western Blot; Sample: Mouse Testis Tissue.

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SIRT1 / Sirtuin 1 Antibody (aa236-490, Biotin) LS-C298039
LifeSpan Biosciences
catalog: LS-C298039
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot

Western blot of recombinant SIRT1 / Sirtuin 1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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SIRT1 / Sirtuin 1 Antibody (aa244-498, Biotin) LS-C301339
LifeSpan Biosciences
catalog: LS-C301339
mouse monoclonal
reactivity: human
conjugate: biotin
application: western blot

Western blot of recombinant SIRT1 / Sirtuin 1. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SIRT1 / Sirtuin 1 Antibody (aa244-498, FITC) LS-C301346
LifeSpan Biosciences
catalog: LS-C301346
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot

Western Blot; Sample: Mouse Testis Tissue.

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SIRT1 / Sirtuin 1 Antibody (aa244-498, FITC) LS-C304190
LifeSpan Biosciences
catalog: LS-C304190
mouse monoclonal
reactivity: human
conjugate: FITC
application: western blot

Western blot of recombinant SIRT1 / Sirtuin 1. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SIRT1 / Sirtuin 1 Antibody LS-C192676
LifeSpan Biosciences
catalog: LS-C192676
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of SIRT1 pAb in extracts from Hela, Jurkat, 293T, MCF7 cells.
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SIRT1 / Sirtuin 1 Antibody (aa456-555, Azide-free) LS-C330660
LifeSpan Biosciences
catalog: LS-C330660
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry
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SIRT1 / Sirtuin 1 Antibody LS-C330804
LifeSpan Biosciences
catalog: LS-C330804
mouse polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of Jurkat cells, using SirT1 antibody. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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SIRT1 / Sirtuin 1 Antibody LS-C330850
LifeSpan Biosciences
catalog: LS-C330850
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) human stomach using SIRT1 antibody at dilution of 1:100 (40x magnification).
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SIRT1 / Sirtuin 1 Antibody LS-C343825
LifeSpan Biosciences
catalog: LS-C343825
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of recombinant human sirtuins, Jurkat cells and HeLa nuclear extract. The nitrocellulose was probed with anti- SIRT1 at 1:1000. The secondary antibody was coupled to alkaline phosphatase and the blot was developed with BCIP/NBT. Arrows indicate positions of molecular weight markers. Lanes: S2, S3, S5 (SIRTs 2, 3, 5, 50 ng each); J (150,000 Jurkat cells); H (18 ug HeLa nuclear extract); S1 (SIRT1- 5 ng, 0.5 ng).

Immunohistochemistry staining of formalin fixed, paraffin-embedded human liver tumor tissue using SIRT1 antibody showing nuclear staining of hepatocytes.
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SIRT1 / Sirtuin 1 Antibody (aa112-311) LS-C344018
LifeSpan Biosciences
catalog: LS-C344018
domestic rabbit polyclonal
reactivity: human
application: western blot

SIRT1 antibody Western blot. All lanes: Anti SIRT1 at 0.5 ug/ml. Lane 1: HEPG2 Whole Cell Lysate at 40 ug. Lane 2: MCF Whole Cell Lysate at 40 ug. Lane 3: SW620 Cell Lysate at 40 ug. Predicted band size: 120 kD . Observed band size: 120 kD.
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SIRT1 / Sirtuin 1 Antibody (aa1-180, clone 1089CT5.3.1) LS-C344127
LifeSpan Biosciences
catalog: LS-C344127
mouse monoclonal (1089CT5.3.1)
reactivity: human
application: western blot

Western blot of lysates from A549, 293 cell line (from left to right) with RPS6KB2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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SIRT1 / Sirtuin 1 Antibody (aa566-601) LS-C344646
LifeSpan Biosciences
catalog: LS-C344646
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry

Fluorescent image of A549 cells stained with Mouse Sirt1 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot of lysate from mouse F9 cell line with Mouse Sirt1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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SIRT1 / Sirtuin 1 Antibody LS-C344838
LifeSpan Biosciences
catalog: LS-C344838
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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SIRT1 / Sirtuin 1 Antibody (aa686-721) LS-C356392
LifeSpan Biosciences
catalog: LS-C356392
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of lysate from mouse NIH/3T3 cell line, using Sirt1 antibody diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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SIRT1 / Sirtuin 1 Antibody (aa587-621) LS-C356624
LifeSpan Biosciences
catalog: LS-C356624
domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-SIRT1 Antibody at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: HeLa whole cell lysates Lane 3: A549 whole cell lysates Lane 4: human testis lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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SIRT1 / Sirtuin 1 Antibody (clone 1F3) LS-C359279
LifeSpan Biosciences
catalog: LS-C359279
mouse monoclonal (1F3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
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SIRT1 / Sirtuin 1 Antibody (aa400-450) LS-C359428
LifeSpan Biosciences
catalog: LS-C359428
domestic rabbit polyclonal
reactivity: human
application: western blot
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SIRT1 / Sirtuin 1 Antibody (30-110 aa) LS-C381089
LifeSpan Biosciences
catalog: LS-C381089
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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SIRT1 / Sirtuin 1 Antibody (30-110 aa) LS-C386081
LifeSpan Biosciences
catalog: LS-C386081
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of SIRT1 antibody
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SIRT1 / Sirtuin 1 Antibody (phospho-Ser47) LS-C416846
LifeSpan Biosciences
catalog: LS-C416846
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of SirT1 phosphorylation expression in JK whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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