SIRT3 / Sirtuin 3 Antibody LS-C75838
LifeSpan Biosciences
catalog: LS-C75838
domestic rabbit monoclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

Western blot of various cell extracts using SIRT 3 (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3).
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SIRT3 / Sirtuin 3 Antibody (C-Terminus) LS-C46967
LifeSpan Biosciences
catalog: LS-C46967
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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SIRT3 / Sirtuin 3 Antibody LS-C10182
LifeSpan Biosciences
catalog: LS-C10182
mouse monoclonal
reactivity: human
application: ELISA
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SIRT3 / Sirtuin 3 Antibody (aa250-279) LS-C100489
LifeSpan Biosciences
catalog: LS-C100489
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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SIRT3 / Sirtuin 3 Antibody (aa152-201) LS-C31604
LifeSpan Biosciences
catalog: LS-C31604
domestic rabbit polyclonal
reactivity: human
application: western blot
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SIRT3 / Sirtuin 3 Antibody (C-Terminus) LS-C31605
LifeSpan Biosciences
catalog: LS-C31605
domestic rabbit polyclonal
reactivity: human
application: western blot
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SIRT3 / Sirtuin 3 Antibody (aa388-399) LS-C55469
LifeSpan Biosciences
catalog: LS-C55469
domestic goat polyclonal
reactivity: human
application: western blot

Antibody (0.2 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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SIRT3 / Sirtuin 3 Antibody LS-C115841
LifeSpan Biosciences
catalog: LS-C115841
chicken polyclonal
reactivity: human
application: western blot, ELISA

Western blot analysis of SIRT3 in mouse heart tissue lysate with SIRT3 antibody at (A) 1 and (B) 2 ug/ml.
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SIRT3 / Sirtuin 3 Antibody (aa170-183) LS-C139812
LifeSpan Biosciences
catalog: LS-C139812
domestic goat polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry

SIRT3 / Sirtuin 3 antibody (0.3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

SIRT3 / Sirtuin 3 antibody flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton, showing a low level of staining. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).

SIRT3 / Sirtuin 3 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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SIRT3 / Sirtuin 3 Antibody LS-C153166
LifeSpan Biosciences
catalog: LS-C153166
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ SIRT3 / Sirtuin 3 Antibody (C-Terminus) LS-B8047
LifeSpan Biosciences
catalog: LS-B8047
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-SIRT3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SIRT3 / Sirtuin 3 Antibody (aa56-84) LS-C161103
LifeSpan Biosciences
catalog: LS-C161103
domestic rabbit polyclonal
reactivity: human
application: western blot

SIRT3 Antibody western blot of MDA-MB453 cell line lysates (35 ug/lane). The SIRT3 antibody detected the SIRT3 protein (arrow).
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SIRT3 / Sirtuin 3 Antibody (aa304-334) LS-C164578
LifeSpan Biosciences
catalog: LS-C164578
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of lysate from mouse heart tissue lysate, using Mouse Sirt3 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Sirt3 Antibody IHC of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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SIRT3 / Sirtuin 3 Antibody (aa387) LS-C190247
LifeSpan Biosciences
catalog: LS-C190247
domestic rabbit polyclonal
reactivity: human
application: western blot
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SIRT3 / Sirtuin 3 Antibody (aa346-395) LS-C200672
LifeSpan Biosciences
catalog: LS-C200672
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HepG2 cells using SIRT3 antibody.
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SIRT3 / Sirtuin 3 Antibody (aa304-334, APC) LS-C217508
LifeSpan Biosciences
catalog: LS-C217508
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa304-334, AP) LS-C217509
LifeSpan Biosciences
catalog: LS-C217509
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa304-334, Biotin) LS-C217510
LifeSpan Biosciences
catalog: LS-C217510
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa304-334, FITC) LS-C217511
LifeSpan Biosciences
catalog: LS-C217511
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa304-334, Azide-free, HRP) LS-C217512
LifeSpan Biosciences
catalog: LS-C217512
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa304-334, PE) LS-C217513
LifeSpan Biosciences
catalog: LS-C217513
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa56-84, AP) LS-C217514
LifeSpan Biosciences
catalog: LS-C217514
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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SIRT3 / Sirtuin 3 Antibody (aa56-84, APC) LS-C217515
LifeSpan Biosciences
catalog: LS-C217515
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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SIRT3 / Sirtuin 3 Antibody (aa56-84, Biotin) LS-C217516
LifeSpan Biosciences
catalog: LS-C217516
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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SIRT3 / Sirtuin 3 Antibody (aa56-84, FITC) LS-C217517
LifeSpan Biosciences
catalog: LS-C217517
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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SIRT3 / Sirtuin 3 Antibody (aa56-84, Azide-free, HRP) LS-C217518
LifeSpan Biosciences
catalog: LS-C217518
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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SIRT3 / Sirtuin 3 Antibody (aa56-84, PE) LS-C217519
LifeSpan Biosciences
catalog: LS-C217519
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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SIRT3 / Sirtuin 3 Antibody (aa250-279, AP) LS-C220673
LifeSpan Biosciences
catalog: LS-C220673
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa250-279, APC) LS-C220674
LifeSpan Biosciences
catalog: LS-C220674
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa250-279, Biotin) LS-C220675
LifeSpan Biosciences
catalog: LS-C220675
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa250-279, FITC) LS-C220676
LifeSpan Biosciences
catalog: LS-C220676
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa250-279, Azide-free, HRP) LS-C220677
LifeSpan Biosciences
catalog: LS-C220677
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa250-279, PE) LS-C220678
LifeSpan Biosciences
catalog: LS-C220678
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa126-382) LS-C292516
LifeSpan Biosciences
catalog: LS-C292516
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western blot of recombinant SIRT3 / Sirtuin 3. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SIRT3 / Sirtuin 3 Antibody (aa126-382, Biotin) LS-C298037
LifeSpan Biosciences
catalog: LS-C298037
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western blot of recombinant SIRT3 / Sirtuin 3. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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SIRT3 / Sirtuin 3 Antibody (aa127-303, Biotin) LS-C298041
LifeSpan Biosciences
catalog: LS-C298041
domestic rabbit polyclonal
reactivity: human, rat
conjugate: biotin
application: western blot

Western blot of recombinant SIRT3 / Sirtuin 3. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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SIRT3 / Sirtuin 3 Antibody (aa126-399, Biotin) LS-C299182
LifeSpan Biosciences
catalog: LS-C299182
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western blot of recombinant SIRT3 / Sirtuin 3. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SIRT3 / Sirtuin 3 Antibody (aa126-382, Biotin) LS-C299291
LifeSpan Biosciences
catalog: LS-C299291
mouse monoclonal
reactivity: human
conjugate: biotin
application: western blot, immunocytochemistry, immunoprecipitation

Western blot of recombinant SIRT3 / Sirtuin 3. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SIRT3 / Sirtuin 3 Antibody (aa126-382, FITC) LS-C301813
LifeSpan Biosciences
catalog: LS-C301813
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot

Western blot of recombinant SIRT3 / Sirtuin 3. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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SIRT3 / Sirtuin 3 Antibody (aa126-399, FITC) LS-C302554
LifeSpan Biosciences
catalog: LS-C302554
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot

Western blot of recombinant SIRT3 / Sirtuin 3. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SIRT3 / Sirtuin 3 Antibody (aa126-382, FITC) LS-C303496
LifeSpan Biosciences
catalog: LS-C303496
mouse monoclonal
reactivity: human
conjugate: FITC
application: western blot, immunocytochemistry, immunoprecipitation

Western blot of recombinant SIRT3 / Sirtuin 3. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SIRT3 / Sirtuin 3 Antibody (aa250-279) LS-C328673
LifeSpan Biosciences
catalog: LS-C328673
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa304-334) LS-C330136
LifeSpan Biosciences
catalog: LS-C330136
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa56-84) LS-C330139
LifeSpan Biosciences
catalog: LS-C330139
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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SIRT3 / Sirtuin 3 Antibody LS-C334057
LifeSpan Biosciences
catalog: LS-C334057
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various tissues, using SIRT3 antibody.

Immunohistochemistry of paraffin-embedded human prostate.
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SIRT3 / Sirtuin 3 Antibody LS-C341794
LifeSpan Biosciences
catalog: LS-C341794
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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SIRT3 / Sirtuin 3 Antibody (aa66-399) LS-C344021
LifeSpan Biosciences
catalog: LS-C344021
domestic rabbit polyclonal
reactivity: human
application: western blot

SIRT3 antibody Western blot. All lanes: Anti SIRT3 at 0.5 ug/ml. WB: Recombinant Human SIRT3 Protein 0.5ng. Predicted band size: 39 kD. Observed band size: 39 kD.
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SIRT3 / Sirtuin 3 Antibody (N-Terminus) LS-C345732
LifeSpan Biosciences
catalog: LS-C345732
domestic rabbit polyclonal
reactivity: human
application: western blot
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SIRT3 / Sirtuin 3 Antibody LS-C346370
LifeSpan Biosciences
catalog: LS-C346370
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of MCF7 cell using SIRT3 antibody. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded rat kidney tissue.
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SIRT3 / Sirtuin 3 Antibody (C-Terminus) LS-C358865
LifeSpan Biosciences
catalog: LS-C358865
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of SIRT3 expression in HEK293T (A); HepG2 (B) whole cell lysates.
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SIRT3 / Sirtuin 3 Antibody (aa20-130) LS-C379892
LifeSpan Biosciences
catalog: LS-C379892
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody at 1 ug/ml+HepG2 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 44 kDa. Observed band size: 44 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
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SIRT3 / Sirtuin 3 Antibody (aa20-130) LS-C379893
LifeSpan Biosciences
catalog: LS-C379893
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody at 2 ug/ml. Lane 1: NIH3T3 whole cell lysate. Lane 2: HepG2 whole cell lysate. secondary Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 44 kDa. Observed band size: 44 kDa.

Immunohistochemistry of paraffin-embedded human pancreas using antibody at 1:100 dilution.
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SIRT3 / Sirtuin 3 Antibody (320-400 aa, C-terminal) LS-C386088
LifeSpan Biosciences
catalog: LS-C386088
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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IHC-plus™ SIRT3 / Sirtuin 3 Antibody LS-B13905
LifeSpan Biosciences
catalog: LS-B13905
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of extracts of various cell lines, using SIRT3 antibody.

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
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