LifeSpan Biosciences FMRP antibody

FMR1 / FMRP Antibody (N-Terminus, clone 1C3) LS-C15265
LifeSpan Biosciences
catalog: LS-C15265

mouse monoclonal (1C3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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FMR1 / FMRP Antibody (clone 4G9) LS-C108759
LifeSpan Biosciences
catalog: LS-C108759

mouse monoclonal (4G9)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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IHC-plus™ FMR1 / FMRP Antibody (aa116-130) LS-B3970
LifeSpan Biosciences
catalog: LS-B3970

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody (0.3 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-FMR1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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FMR1 / FMRP Antibody (N-Terminus, clone 6A15) LS-C82231
LifeSpan Biosciences
catalog: LS-C82231

mouse monoclonal (6A15)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation

Immunoprecipitation - Drosophila FMR1 antibody [6A15] using tissue lysate from Drosophila cellularizing embryos
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IHC-plus™ FMR1 / FMRP Antibody (aa594-607) LS-B3953
LifeSpan Biosciences
catalog: LS-B3953

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

FMR1 / FMRP antibody (0.1µg/ml) staining of KELLY cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-FMR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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FMR1 / FMRP Antibody LS-C121832
LifeSpan Biosciences
catalog: LS-C121832

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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FMR1 / FMRP Antibody LS-C129158
LifeSpan Biosciences
catalog: LS-C129158

domestic rabbit polyclonal
reactivity: human
application: western blot

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FMR1 / FMRP Antibody (aa421-470) LS-C135098
LifeSpan Biosciences
catalog: LS-C135098

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

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FMR1 / FMRP Antibody (aa20-48) LS-C166192
LifeSpan Biosciences
catalog: LS-C166192

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Formalin-fixed and paraffin-embedded human brain tissue reacted with FMR1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot of FMR1 Antibody in mouse lung tissue lysates (35 ug/lane). FMR1 (arrow) was detected using the purified antibody.

FMR1 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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FMR1 / FMRP Antibody (clone OTI1C6) LS-C173739
LifeSpan Biosciences
catalog: LS-C173739

mouse monoclonal (OTI1C6)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-FMR1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FMR1 mouse monoclonal antibody.

Anti-FMR1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FMR1.
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FMR1 / FMRP Antibody (clone OTI2C3) LS-C173741
LifeSpan Biosciences
catalog: LS-C173741

mouse monoclonal (OTI2C3)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Human breast tissue using anti-FMR1 mouse monoclonal antibody.
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FMR1 / FMRP Antibody (clone OTI1H9) LS-C173769
LifeSpan Biosciences
catalog: LS-C173769

mouse monoclonal (OTI1H9)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FMR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FMR1.
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FMR1 / FMRP Antibody (clone OTI3B4) LS-C173787
LifeSpan Biosciences
catalog: LS-C173787

mouse monoclonal (OTI3B4)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Human Kidney tissue using anti-FMR1 mouse monoclonal antibody.

Anti-FMR1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FMR1.
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FMR1 / FMRP Antibody (clone 2D4) LS-C197237
LifeSpan Biosciences
catalog: LS-C197237

mouse monoclonal (2D4)
reactivity: human
application: western blot, ELISA

Western Blot analysis of FMR1 expression in transfected 293T cell line by FMR1 monoclonal antibody (M01), clone 2D4.Lane 1: FMR1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged FMR1 is approximately 0.1 ng/ml as a capture antibody.

Western blot analysis of FMR1 over-expressed 293 cell line, cotransfected with FMR1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FMR1 monoclonal antibody (M01), clone 2D4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
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FMR1 / FMRP Antibody (C-Terminus) LS-C201385
LifeSpan Biosciences
catalog: LS-C201385

domestic rabbit polyclonal
reactivity: human
application: western blot

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FMR1 / FMRP Antibody (aa20-48, AP) LS-C206636
LifeSpan Biosciences
catalog: LS-C206636

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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FMR1 / FMRP Antibody (aa20-48, FITC) LS-C206637
LifeSpan Biosciences
catalog: LS-C206637

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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FMR1 / FMRP Antibody (aa20-48, Biotin) LS-C206638
LifeSpan Biosciences
catalog: LS-C206638

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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FMR1 / FMRP Antibody (aa20-48, APC) LS-C206639
LifeSpan Biosciences
catalog: LS-C206639

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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FMR1 / FMRP Antibody (aa20-48, PE) LS-C206641
LifeSpan Biosciences
catalog: LS-C206641

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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FMR1 / FMRP Antibody (aa20-48, Azide-free, HRP) LS-C209094
LifeSpan Biosciences
catalog: LS-C209094

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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IHC-plus™ FMR1 / FMRP Antibody (clone 1D10) LS-B9991
LifeSpan Biosciences
catalog: LS-B9991

mouse monoclonal (1D10)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-FMR1 / FMRP antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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IHC-plus™ FMR1 / FMRP Antibody LS-B10188
LifeSpan Biosciences
catalog: LS-B10188

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence of FMR1 in human brain tissue with FMR1 antibody at 20 µg/mL.

Anti-FMR1 / FMRP antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot analysis of FMR1 in rat brain tissue lysate with FMR1 antibody at 1 ug/ml.
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FMR1 / FMRP Antibody (aa20-48) LS-C327398
LifeSpan Biosciences
catalog: LS-C327398

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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FMR1 / FMRP Antibody LS-C334188
LifeSpan Biosciences
catalog: LS-C334188

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded rat brain tissue.

Immunofluorescence analysis of U2OS cells.
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FMR1 / FMRP Antibody (aa36-279, clone OTI5B9) LS-C337652
LifeSpan Biosciences
catalog: LS-C337652

mouse monoclonal (OTI5B9)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Human Kidney tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Anti-FMR1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FMR1.
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FMR1 / FMRP Antibody LS-C341686
LifeSpan Biosciences
catalog: LS-C341686

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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FMR1 / FMRP Antibody LS-C482477
LifeSpan Biosciences
catalog: LS-C482477

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of FMR1 expression in MCF7 (A); Jurkat (B); mouse kidney (C); mouse liver (D) whole cell lysates.

Immunohistochemical analysis of FMR1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of FMR1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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