IHC-plus™ APG4B / ATG4B Antibody LS-B1812
LifeSpan Biosciences
catalog: LS-B1812

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATG4B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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APG4B / ATG4B Antibody (C-Terminus) LS-C29815
LifeSpan Biosciences
catalog: LS-C29815

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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APG4B / ATG4B Antibody (C-Terminus) LS-C31597
LifeSpan Biosciences
catalog: LS-C31597

domestic rabbit polyclonal
reactivity: human
application: western blot

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APG4B / ATG4B Antibody LS-C47395
LifeSpan Biosciences
catalog: LS-C47395

domestic rabbit polyclonal
reactivity: human
application: western blot

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APG4B / ATG4B Antibody (N-Terminus) LS-C69118
LifeSpan Biosciences
catalog: LS-C69118

domestic rabbit polyclonal
reactivity: human
application: ELISA

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APG4B / ATG4B Antibody (aa72-121) LS-C81992
LifeSpan Biosciences
catalog: LS-C81992

domestic rabbit polyclonal
reactivity: human
application: western blot

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APG4B / ATG4B Antibody (aa71-120) LS-C119347
LifeSpan Biosciences
catalog: LS-C119347

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using ATG4B Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using ATG4B Antibody. The lane on the right is blocked with the synthesized peptide.
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APG4B / ATG4B Antibody (aa258-284) LS-C156588
LifeSpan Biosciences
catalog: LS-C156588

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of APG4B (arrow) using purified antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APG4B gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Autophagy APG4B Antibody (E273), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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APG4B / ATG4B Antibody (aa16-45) LS-C156593
LifeSpan Biosciences
catalog: LS-C156593

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-hAPG4B-R31 antibody in 293 cell line lysates transiently transfected with the ATG4B gene (2 ug/lane). hAPG4B-R31(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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APG4B / ATG4B Antibody (aa239-265) LS-C159088
LifeSpan Biosciences
catalog: LS-C159088

domestic rabbit polyclonal
reactivity: human
application: western blot

APG4B Antibody (Center G254)western blot of Uterus tissue lysates (35 ug/lane). The APG4B antibody detected the APG4B protein (arrow).
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APG4B / ATG4B Antibody (aa358-390) LS-C156592
LifeSpan Biosciences
catalog: LS-C156592

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Fluorescent image of U251 cells stained with ATG4B antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG4B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Western blot of anti-hAPG4B-373 antibody in 293 cell line lysates transiently transfected with the ATG4B gene (2 ug/lane). hAPG4B-373(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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APG4B / ATG4B Antibody (aa239-265, AP) LS-C251502
LifeSpan Biosciences
catalog: LS-C251502

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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APG4B / ATG4B Antibody (aa239-265, APC) LS-C251503
LifeSpan Biosciences
catalog: LS-C251503

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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APG4B / ATG4B Antibody (aa239-265, FITC) LS-C251505
LifeSpan Biosciences
catalog: LS-C251505

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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APG4B / ATG4B Antibody (aa239-265, Azide-free, HRP) LS-C251506
LifeSpan Biosciences
catalog: LS-C251506

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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APG4B / ATG4B Antibody (aa239-265, PE) LS-C251507
LifeSpan Biosciences
catalog: LS-C251507

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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APG4B / ATG4B Antibody (aa258-284, AP) LS-C251932
LifeSpan Biosciences
catalog: LS-C251932

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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APG4B / ATG4B Antibody (aa258-284, Biotin) LS-C251933
LifeSpan Biosciences
catalog: LS-C251933

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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APG4B / ATG4B Antibody (aa258-284, APC) LS-C251934
LifeSpan Biosciences
catalog: LS-C251934

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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APG4B / ATG4B Antibody (aa258-284, Azide-free, HRP) LS-C251935
LifeSpan Biosciences
catalog: LS-C251935

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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APG4B / ATG4B Antibody (aa258-284, FITC) LS-C251936
LifeSpan Biosciences
catalog: LS-C251936

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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APG4B / ATG4B Antibody (aa258-284, PE) LS-C251937
LifeSpan Biosciences
catalog: LS-C251937

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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APG4B / ATG4B Antibody (aa239-265, Biotin) LS-C252867
LifeSpan Biosciences
catalog: LS-C252867

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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IHC-plus™ APG4B / ATG4B Antibody LS-B10684
LifeSpan Biosciences
catalog: LS-B10684

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-APG4B / ATG4B antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

ATG4B/MB10290 antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ATG4B/MB10290 antibody detected the ATG4B/MB10290 protein (arrow).
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APG4B / ATG4B Antibody (aa350-400) LS-C286143
LifeSpan Biosciences
catalog: LS-C286143

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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APG4B / ATG4B Antibody (aa275-325) LS-C289609
LifeSpan Biosciences
catalog: LS-C289609

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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APG4B / ATG4B Antibody (aa239-265) LS-C319527
LifeSpan Biosciences
catalog: LS-C319527

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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APG4B / ATG4B Antibody (aa258-284) LS-C319674
LifeSpan Biosciences
catalog: LS-C319674

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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IHC-plus™ APG4B / ATG4B Antibody (aa110-159) LS-B11540
LifeSpan Biosciences
catalog: LS-B11540

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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APG4B / ATG4B Antibody (aa250-290) LS-C344196
LifeSpan Biosciences
catalog: LS-C344196

domestic rabbit polyclonal
reactivity: human
application: western blot

ATG4B Antibody western blot of Jurkat cell line lysates (35 ug/lane). The ATG4B antibody detected the ATG4B protein (arrow).
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APG4B / ATG4B Antibody (aa1-30) LS-C344682
LifeSpan Biosciences
catalog: LS-C344682

domestic rabbit polyclonal
reactivity: human, zebrafish
application: western blot

DANRE atg4b Antibody western blot of zebra fish brain tissue lysates (35 ug/lane). The DANRE atg4b antibody detected the DANRE atg4b protein (arrow).
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IHC-plus™ APG4B / ATG4B Antibody LS-B12106
LifeSpan Biosciences
catalog: LS-B12106

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 ug/ml.
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APG4B / ATG4B Antibody (Internal) LS-C354558
LifeSpan Biosciences
catalog: LS-C354558

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of ATG4B expression in Jurkat (A) whole cell lysates.

Immunohistochemical analysis of ATG4B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
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APG4B / ATG4B Antibody (C-Terminus) LS-C357715
LifeSpan Biosciences
catalog: LS-C357715

domestic rabbit polyclonal
reactivity: human
application: western blot

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APG4B / ATG4B Antibody (N-Terminus) LS-C357717
LifeSpan Biosciences
catalog: LS-C357717

domestic rabbit polyclonal
reactivity: human
application: western blot

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APG4B / ATG4B Antibody (aa1-300) LS-C358144
LifeSpan Biosciences
catalog: LS-C358144

domestic rabbit polyclonal
reactivity: human
application: western blot

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APG4B / ATG4B Antibody (aa150-393) LS-C358145
LifeSpan Biosciences
catalog: LS-C358145

domestic rabbit polyclonal
reactivity: human
application: western blot

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APG4B / ATG4B Antibody (40-120 aa, Internal) LS-C382187
LifeSpan Biosciences
catalog: LS-C382187

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Atg4b antibody
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