IHC-plus™ AKR1B1 / Aldose Reductase Antibody (N-Terminus) LS-B5811
LifeSpan Biosciences
catalog: LS-B5811
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-AKR1B1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
quantity:
price:
to the supplier
IHC-plus™ AKR1B1 / Aldose Reductase Antibody (clone 2D12) LS-B6072
LifeSpan Biosciences
catalog: LS-B6072
mouse monoclonal (2D12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Detection limit for recombinant GST tagged AKR1B1 is 1 ng/ml as a capture antibody.

Anti-AKR1B1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody (aa290-316) LS-C98606
LifeSpan Biosciences
catalog: LS-C98606
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

AKR1B1 Antibody western blot of A375 cell line and human placenta tissue lysates (35 ug/lane). The AKR1B1 antibody detected the AKR1B1 protein (arrow).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescence of AKR1B1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody (aa201-250) LS-C81174
LifeSpan Biosciences
catalog: LS-C81174
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody (aa102-135) LS-C98607
LifeSpan Biosciences
catalog: LS-C98607
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.

Confocal immunofluorescence of AKR1B1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody LS-C109517
LifeSpan Biosciences
catalog: LS-C109517
domestic rabbit polyclonal
reactivity: human
application: western blot

Sample (30 ug of whole cell lysate). A: 293T. B: A431. 10% SDS PAGE. AKR1B1 antibody diluted at 1:1000.
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody (Lys275) LS-C177349
LifeSpan Biosciences
catalog: LS-C177349
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Aldose Reductase (K275) pAb in extracts from HUVEC cells.

IHC of Aldose Reductase (K275) pAb in paraffin-embedded human lung carcinoma tissue.
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody LS-C178285
LifeSpan Biosciences
catalog: LS-C178285
mouse monoclonal
reactivity: human
application: western blot

Western blot detection of AKR1B1 in HT1080,MCF7,A431&Hela cell lysates using AKR1B1 antibody (1:1000 diluted).
quantity:
price:
to the supplier
IHC-plus™ AKR1B1 / Aldose Reductase Antibody (aa241-290) LS-B9253
LifeSpan Biosciences
catalog: LS-B9253
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-AKR1B1 / Aldose Reductase antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot analysis of lysates from HUVEC cells, using AKR1B1 Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody (aa226-275) LS-C200112
LifeSpan Biosciences
catalog: LS-C200112
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HUVECcells using AKR1B1 antibody.
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody LS-C306181
LifeSpan Biosciences
catalog: LS-C306181
mouse polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody LS-C309387
LifeSpan Biosciences
catalog: LS-C309387
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody LS-C192693
LifeSpan Biosciences
catalog: LS-C192693
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of human liver cell lines, using AKR1B1 antibody.
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody LS-C331634
LifeSpan Biosciences
catalog: LS-C331634
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using AKR1B1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody (C-Terminus) LS-C347626
LifeSpan Biosciences
catalog: LS-C347626
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody LS-C347630
LifeSpan Biosciences
catalog: LS-C347630
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody (C-Terminus) LS-C353884
LifeSpan Biosciences
catalog: LS-C353884
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Aldose Reductase expression in Jurkat (A); HeLa (B); SP2/0 (C); PC12 (D) whole cell lysates.

Immunohistochemical analysis of Aldose Reductase staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Aldose Reductase staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
AKR1B1 / Aldose Reductase Antibody (210-290 aa, C-terminal) LS-C381986
LifeSpan Biosciences
catalog: LS-C381986
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Aldose Reductase antibody
quantity:
price:
to the supplier