IHC-plus™ ATF6 Antibody LS-B2430
LifeSpan Biosciences
catalog: LS-B2430
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.
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IHC-plus™ ATF6 Antibody LS-B2516
LifeSpan Biosciences
catalog: LS-B2516
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-ATF6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/ml.

Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/ml.
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ATF6 Antibody (C-Terminus) LS-C3517
LifeSpan Biosciences
catalog: LS-C3517
domestic rabbit polyclonal
reactivity: human
application: western blot
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ATF6 Antibody (aa36-85) LS-C109612
LifeSpan Biosciences
catalog: LS-C109612
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ ATF6 Antibody (C-Terminus) LS-B2388
LifeSpan Biosciences
catalog: LS-B2388
domestic goat polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATF6 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ ATF6 Antibody (C-Terminus) LS-B5587
LifeSpan Biosciences
catalog: LS-B5587
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATF6 antibody IHC of mouse adipose. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ATF6 Antibody LS-C136870
LifeSpan Biosciences
catalog: LS-C136870
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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IHC-plus™ ATF6 Antibody (clone 3D5) LS-B6032
LifeSpan Biosciences
catalog: LS-B6032
mouse monoclonal (3D5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATF6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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ATF6 Antibody (aa350-377) LS-C156565
LifeSpan Biosciences
catalog: LS-C156565
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of ATF6 Antibody in A2058 cell line lysates (35 ug/lane). ATF6 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ATF6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ATF6 Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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ATF6 Antibody (N-Terminus, Magnetic beads) LS-C171662
LifeSpan Biosciences
catalog: LS-C171662
domestic rabbit polyclonal
reactivity: human
conjugate: Bead
application: immunoprecipitation
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ATF6 Antibody (N-Terminus, Sepharose) LS-C171663
LifeSpan Biosciences
catalog: LS-C171663
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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ATF6 Antibody LS-C180124
LifeSpan Biosciences
catalog: LS-C180124
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
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ATF6 Antibody (DY488) LS-C180694
LifeSpan Biosciences
catalog: LS-C180694
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
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ATF6 Antibody (DY550) LS-C180695
LifeSpan Biosciences
catalog: LS-C180695
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
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ATF6 Antibody (clone 47C6a) LS-C184793
LifeSpan Biosciences
catalog: LS-C184793
mouse monoclonal (47C6a)
reactivity: human
application: immunocytochemistry
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ATF6 Antibody (aa437-449) LS-C186444
LifeSpan Biosciences
catalog: LS-C186444
domestic goat polyclonal
reactivity: human
application: western blot

ATF6 antibody (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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ATF6 Antibody (aa350-377, AP) LS-C206373
LifeSpan Biosciences
catalog: LS-C206373
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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ATF6 Antibody (aa350-377, APC) LS-C206374
LifeSpan Biosciences
catalog: LS-C206374
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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ATF6 Antibody (aa350-377, FITC) LS-C206375
LifeSpan Biosciences
catalog: LS-C206375
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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ATF6 Antibody (aa350-377, PE) LS-C206376
LifeSpan Biosciences
catalog: LS-C206376
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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ATF6 Antibody (aa350-377, Azide-free, HRP) LS-C207933
LifeSpan Biosciences
catalog: LS-C207933
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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ATF6 Antibody (aa125-175) LS-C290376
LifeSpan Biosciences
catalog: LS-C290376
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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ATF6 Antibody (aa620-670) LS-C290377
LifeSpan Biosciences
catalog: LS-C290377
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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ATF6 Antibody (aa423-646) LS-C292619
LifeSpan Biosciences
catalog: LS-C292619
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant ATF6. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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ATF6 Antibody LS-C306425
LifeSpan Biosciences
catalog: LS-C306425
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry
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ATF6 Antibody LS-C309543
LifeSpan Biosciences
catalog: LS-C309543
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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ATF6 Antibody LS-C192659
LifeSpan Biosciences
catalog: LS-C192659
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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ATF6 Antibody (aa350-377) LS-C326783
LifeSpan Biosciences
catalog: LS-C326783
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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ATF6 Antibody LS-C330827
LifeSpan Biosciences
catalog: LS-C330827
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cell lines, using ATF6 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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ATF6 Antibody (clone 47C6a) LS-C342810
LifeSpan Biosciences
catalog: LS-C342810
mouse monoclonal (47C6a)
reactivity: human
application: western blot, immunocytochemistry

Left : Western blot analysis of immunized recombinant protein, using anti-ATF6 monoclonal antibody. Right : CBB staining of immunized recombinant protein.
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ATF6 Antibody (N-Terminus, clone AF6229) LS-C357712
LifeSpan Biosciences
catalog: LS-C357712
mouse monoclonal (AF6229)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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ATF6 Antibody (aa1-400, clone ABM1A40) LS-C358236
LifeSpan Biosciences
catalog: LS-C358236
mouse monoclonal (ABM1A40)
reactivity: human
application: western blot
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IHC-plus™ ATF6 Antibody (N-Terminus) LS-B13270
LifeSpan Biosciences
catalog: LS-B13270
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
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ATF6 Antibody (aa1-377) LS-C372729
LifeSpan Biosciences
catalog: LS-C372729
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of ATF6 antibody.

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ATF6 Antibody LS-C482243
LifeSpan Biosciences
catalog: LS-C482243
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of ATF6 expression in K562 (A); PC3 (B); mouse heart (C) whole cell lysates.

Immunofluorescent analysis of ATF6 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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