SLIM / FHL1 Antibody (aa207-256) LS-C30688
LifeSpan Biosciences
catalog: LS-C30688

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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IHC-plus™ SLIM / FHL1 Antibody (aa51-100) LS-B3484
LifeSpan Biosciences
catalog: LS-B3484

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-FHL1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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IHC-plus™ SLIM / FHL1 Antibody (aa261-272) LS-B2495
LifeSpan Biosciences
catalog: LS-B2495

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody staining (0.05 ug/ml) of Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-FHL1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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SLIM / FHL1 Antibody (aa233-246) LS-C55478
LifeSpan Biosciences
catalog: LS-C55478

domestic goat polyclonal
reactivity: human
application: western blot

Antibody (0.01 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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SLIM / FHL1 Antibody (aa214-240) LS-C166150
LifeSpan Biosciences
catalog: LS-C166150

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of FHL1 (arrow) using rabbit polyclonal FHL1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FHL1 gene.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with FHL1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of widr cells using FHL1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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SLIM / FHL1 Antibody (clone OTI2E1) LS-C115289
LifeSpan Biosciences
catalog: LS-C115289

mouse monoclonal (OTI2E1)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.

Anti-FHL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FHL1 antibody, and then analyzed by flow cytometry.
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SLIM / FHL1 Antibody (clone OTI1D6) LS-C115303
LifeSpan Biosciences
catalog: LS-C115303

mouse monoclonal (OTI1D6)
reactivity: human
application: western blot, flow cytometry

Western blot of extracts (35 ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FHL1 antibody, and then analyzed by flow cytometry.
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SLIM / FHL1 Antibody (clone OTI3H3) LS-C115366
LifeSpan Biosciences
catalog: LS-C115366

mouse monoclonal (OTI3H3)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.

Anti-FHL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FHL1 antibody, and then analyzed by flow cytometry.
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SLIM / FHL1 Antibody (clone OTI2E11) LS-C172314
LifeSpan Biosciences
catalog: LS-C172314

mouse monoclonal (OTI2E11)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot of extracts (35 ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.

Anti-FHL1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FHL1 antibody, and then analyzed by flow cytometry.
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SLIM / FHL1 Antibody (aa18-261) LS-C179177
LifeSpan Biosciences
catalog: LS-C179177

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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SLIM / FHL1 Antibody (aa93-166) LS-C191445
LifeSpan Biosciences
catalog: LS-C191445

domestic goat polyclonal
reactivity: human
application: western blot

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SLIM / FHL1 Antibody (clone 2A9) LS-C197221
LifeSpan Biosciences
catalog: LS-C197221

mouse monoclonal (2A9)
reactivity: human
application: western blot, ELISA

Western Blot analysis of FHL1 expression in transfected 293T cell line by FHL1 monoclonal antibody (M01), clone 2A9.Lane 1: FHL1 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged FHL1 is approximately 0.3 ng/ml as a capture antibody.
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SLIM / FHL1 Antibody LS-C311143
LifeSpan Biosciences
catalog: LS-C311143

domestic rabbit polyclonal
reactivity: human
application: western blot

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SLIM / FHL1 Antibody LS-C346088
LifeSpan Biosciences
catalog: LS-C346088

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HeLa cell using FHL1 antibody. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cells.
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