IHC-plus™ FHIT Antibody LS-B3166
LifeSpan Biosciences
catalog: LS-B3166

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-FHIT antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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FHIT Antibody (aa81-130) LS-C118449
LifeSpan Biosciences
catalog: LS-C118449

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of A549 cells, using FHIT Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FHIT Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using FHIT Antibody. The lane on the right is blocked with the synthesized peptide.
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FHIT Antibody (C-Terminus) LS-C116412
LifeSpan Biosciences
catalog: LS-C116412

domestic rabbit polyclonal
reactivity: human
application: western blot

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FHIT Antibody (Glu116) LS-C176023
LifeSpan Biosciences
catalog: LS-C176023

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of FHIT (E116) pAb in extracts from A549 cells.

IHC of FHIT (E116) pAb in paraffin-embedded human breast carcinoma tissue.
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FHIT Antibody (clone 1C3) LS-C197220
LifeSpan Biosciences
catalog: LS-C197220

mouse monoclonal (1C3)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged FHIT is approximately 0.1 ng/ml as a capture antibody.
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FHIT Antibody (phospho-Tyr114) LS-C198960
LifeSpan Biosciences
catalog: LS-C198960

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using FHIT (Phospho-Tyr114) Antibody. The picture on the right is blocked with the phospho peptide.
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IHC-plus™ FHIT Antibody LS-B10125
LifeSpan Biosciences
catalog: LS-B10125

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 ug/ml.

Immunofluorescence of FHIT in HeLa cells with FHIT antibody at 5 ug/ml.

Anti-FHIT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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FHIT Antibody LS-C192457
LifeSpan Biosciences
catalog: LS-C192457

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of FHIT pAb in extracts from K562 cells.
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FHIT Antibody LS-C331341
LifeSpan Biosciences
catalog: LS-C331341

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using FHIT antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunohistochemistry of paraffin-embedded rat heart using FHIT antibodyat dilution of 1:100 (40x lens).
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FHIT Antibody (aa1-147) LS-C344033
LifeSpan Biosciences
catalog: LS-C344033

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

FHIT antibody Western blot. All lanes: Anti FHIT at 0.5 ug/ml. Lane 1: HT1080 Whole Cell Lysate at 40 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: Jurkat Whole Cell Lysate at 40 ug. Predicted band size: 17 kD. Observed band size: 17 kD.

FHIT antibody IHC-paraffin: Human Mammary Cancer Tissue.
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FHIT Antibody (C-Terminus) LS-C345642
LifeSpan Biosciences
catalog: LS-C345642

domestic rabbit polyclonal
reactivity: human
application: western blot

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FHIT Antibody (Internal) LS-C352195
LifeSpan Biosciences
catalog: LS-C352195

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of FHIT expression in HEK293T (A) whole cell lysates.

Immunohistochemical analysis of FHIT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of FHIT staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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FHIT Antibody (clone 1093CT23.1.2) LS-C356276
LifeSpan Biosciences
catalog: LS-C356276

mouse monoclonal (1093CT23.1.2)
reactivity: human
application: western blot

All lanes : Anti-FHIT Antibody at 1:4000 dilution Lane 1: HL-60 whole cell lysates Lane 2: MDA-MB-453 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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FHIT Antibody (aa2-150, FITC) LS-C371314
LifeSpan Biosciences
catalog: LS-C371314

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: ELISA

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FHIT Antibody (aa2-150, Biotin) LS-C371315
LifeSpan Biosciences
catalog: LS-C371315

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: ELISA

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FHIT Antibody (aa2-150) LS-C371318
LifeSpan Biosciences
catalog: LS-C371318

domestic rabbit polyclonal
reactivity: human, mouse
application: ELISA

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FHIT Antibody (50-130 aa) LS-C381701
LifeSpan Biosciences
catalog: LS-C381701

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

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FHIT Antibody (60-140 aa, Internal) LS-C383488
LifeSpan Biosciences
catalog: LS-C383488

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Fhit antibody
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FHIT Antibody (50-130 aa) LS-C383491
LifeSpan Biosciences
catalog: LS-C383491

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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FHIT Antibody LS-C404982
LifeSpan Biosciences
catalog: LS-C404982

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

Immunohistochemistry of paraffin-embedded Human cervical cancer using FHIT Polyclonal Antibody at dilution of 1:30.
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