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IHC-plus™ ERBB4 / HER4 Antibody (Internal) LS-A2847
LifeSpan Biosciences
catalog: LS-A2847
domestic rabbit polyclonal
reactivity:
human
application:
immunohistochemistry
,
immunohistochemistry - paraffin section
Anti-ERBB4 antibody IHC of human glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (clone H4.72.8 or AB72, Azide-free) LS-C88052
LifeSpan Biosciences
catalog: LS-C88052
mouse monoclonal (H4.72.8 or AB72)
reactivity:
human
application:
flow cytometry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1276-1308) LS-C97506
LifeSpan Biosciences
catalog: LS-C97506
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunohistochemistry - paraffin section
citations: 1
Western blot of anti-ErbB4 antibody in T-47D cell lysate. ErbB4 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
quantity:
price:
to the supplier
IHC-plus™ ERBB4 / HER4 Antibody (Internal) LS-A2846
LifeSpan Biosciences
catalog: LS-A2846
domestic rabbit polyclonal
reactivity:
human
application:
immunohistochemistry
,
immunohistochemistry - paraffin section
Anti-ERBB4 antibody IHC of human glomerulus and renal tubular epithelium in cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
quantity:
price:
to the supplier
IHC-plus™ ERBB4 / HER4 Antibody (C-Terminus) LS-A3452
LifeSpan Biosciences
catalog: LS-A3452
domestic rabbit polyclonal
reactivity:
human
application:
immunohistochemistry
,
immunohistochemistry - paraffin section
Anti-ERBB4 antibody IHC of human glomerulus and renal tubular epithelium in cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1285-1308) LS-C6701
LifeSpan Biosciences
catalog: LS-C6701
domestic rabbit polyclonal
reactivity:
human
application:
ELISA
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (C-Terminus) LS-C6702
LifeSpan Biosciences
catalog: LS-C6702
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunohistochemistry - paraffin section
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (C-Terminus) LS-C46879
LifeSpan Biosciences
catalog: LS-C46879
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (clone H4.77.16, Azide-free) LS-C88004
LifeSpan Biosciences
catalog: LS-C88004
mouse monoclonal (H4.77.16)
reactivity:
human
application:
immunoprecipitation
,
flow cytometry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (clone H4.72.8) LS-C88050
LifeSpan Biosciences
catalog: LS-C88050
mouse monoclonal (H4.72.8)
reactivity:
human
application:
flow cytometry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (clone H4.72.8, Biotin) LS-C88051
LifeSpan Biosciences
catalog: LS-C88051
mouse monoclonal (H4.72.8)
reactivity:
human
conjugate: biotin
application:
flow cytometry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (clone HFR-1) LS-C88314
LifeSpan Biosciences
catalog: LS-C88314
mouse monoclonal (HFR-1)
reactivity:
human
application:
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (clone HFR-1, Azide-free) LS-C88315
LifeSpan Biosciences
catalog: LS-C88315
mouse monoclonal (HFR-1)
reactivity:
human
application:
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody LS-C88730
LifeSpan Biosciences
catalog: LS-C88730
domestic rabbit polyclonal
reactivity:
human
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (Azide-free) LS-C88731
LifeSpan Biosciences
catalog: LS-C88731
domestic rabbit polyclonal
reactivity:
human
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1162) LS-C96884
LifeSpan Biosciences
catalog: LS-C96884
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Phospho-HER4-Y1162 Antibody was used to detect Phospho-HER4-Y1162 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min Phospho-HER4-Y1162 Antibody was used at 1:750 in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Confocal immunofluorescence of Phospho-HER4-Y1162 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa25-55) LS-C97505
LifeSpan Biosciences
catalog: LS-C97505
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunohistochemistry - paraffin section
Western blot of anti-ErbB4 antibody in HL60 cell lysate. ErbB4 (Arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1250-1299) LS-C117911
LifeSpan Biosciences
catalog: LS-C117911
domestic rabbit polyclonal
reactivity:
human
application:
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Immunofluorescence analysis of HepG2 cells, using HER4 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HER4 Antibody. The picture on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (Extracellular Domain, APC) LS-C125220
LifeSpan Biosciences
catalog: LS-C125220
mouse monoclonal
reactivity:
human
conjugate: APC
application:
flow cytometry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (Extracellular Domain, PE) LS-C125221
LifeSpan Biosciences
catalog: LS-C125221
mouse monoclonal
reactivity:
human
conjugate: PE
application:
flow cytometry
quantity:
price:
to the supplier
IHC-plus™ ERBB4 / HER4 Antibody (Internal) LS-A3451
LifeSpan Biosciences
catalog: LS-A3451
domestic rabbit polyclonal
reactivity:
human
application:
immunohistochemistry
,
immunohistochemistry - paraffin section
Anti-ERBB4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1141-1166) LS-C159823
LifeSpan Biosciences
catalog: LS-C159823
domestic rabbit polyclonal
reactivity:
human
application:
western blot
HER4 Antibody (pY1162) western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The HER4 antibody detected the HER4 protein (arrow).
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (Glu1280) LS-C176638
LifeSpan Biosciences
catalog: LS-C176638
domestic rabbit polyclonal
reactivity:
human
application:
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
IHC of ErbB-4 (E1280) pAb in paraffin-embedded human brain tissue.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1284) LS-C199055
LifeSpan Biosciences
catalog: LS-C199055
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right is blocked with the phospho peptide.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1141-1166, APC) LS-C258412
LifeSpan Biosciences
catalog: LS-C258412
domestic rabbit polyclonal
reactivity:
human
conjugate: APC
application:
western blot
,
ELISA
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1141-1166, AP) LS-C258413
LifeSpan Biosciences
catalog: LS-C258413
domestic rabbit polyclonal
reactivity:
human
conjugate: AP
application:
western blot
,
ELISA
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1141-1166, Biotin) LS-C258414
LifeSpan Biosciences
catalog: LS-C258414
domestic rabbit polyclonal
reactivity:
human
conjugate: biotin
application:
western blot
,
ELISA
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1141-1166, FITC) LS-C258415
LifeSpan Biosciences
catalog: LS-C258415
domestic rabbit polyclonal
reactivity:
human
conjugate: FITC
application:
western blot
,
ELISA
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1141-1166, Azide-free, HRP) LS-C258416
LifeSpan Biosciences
catalog: LS-C258416
domestic rabbit polyclonal
reactivity:
human
conjugate: HRP
application:
western blot
,
ELISA
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1141-1166, PE) LS-C258422
LifeSpan Biosciences
catalog: LS-C258422
domestic rabbit polyclonal
reactivity:
human
conjugate: PE
application:
western blot
,
ELISA
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1162, AP) LS-C277155
LifeSpan Biosciences
catalog: LS-C277155
domestic rabbit polyclonal
reactivity:
human
conjugate: AP
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1162, APC) LS-C277156
LifeSpan Biosciences
catalog: LS-C277156
domestic rabbit polyclonal
reactivity:
human
conjugate: APC
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1162, Biotin) LS-C277157
LifeSpan Biosciences
catalog: LS-C277157
domestic rabbit polyclonal
reactivity:
human
conjugate: biotin
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1162, FITC) LS-C277158
LifeSpan Biosciences
catalog: LS-C277158
domestic rabbit polyclonal
reactivity:
human
conjugate: FITC
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1162, Azide-free, HRP) LS-C277159
LifeSpan Biosciences
catalog: LS-C277159
domestic rabbit polyclonal
reactivity:
human
conjugate: HRP
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1162, PE) LS-C277160
LifeSpan Biosciences
catalog: LS-C277160
domestic rabbit polyclonal
reactivity:
human
conjugate: PE
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1188, AP) LS-C277161
LifeSpan Biosciences
catalog: LS-C277161
domestic rabbit polyclonal
reactivity:
human
conjugate: AP
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1188, Biotin) LS-C277162
LifeSpan Biosciences
catalog: LS-C277162
domestic rabbit polyclonal
reactivity:
human
conjugate: biotin
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1188, APC) LS-C277163
LifeSpan Biosciences
catalog: LS-C277163
domestic rabbit polyclonal
reactivity:
human
conjugate: APC
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1188, FITC) LS-C277164
LifeSpan Biosciences
catalog: LS-C277164
domestic rabbit polyclonal
reactivity:
human
conjugate: FITC
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1188, Azide-free, HRP) LS-C277165
LifeSpan Biosciences
catalog: LS-C277165
domestic rabbit polyclonal
reactivity:
human
conjugate: HRP
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa25-55, AP) LS-C277166
LifeSpan Biosciences
catalog: LS-C277166
domestic rabbit polyclonal
reactivity:
human
conjugate: AP
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa25-55, Biotin) LS-C277167
LifeSpan Biosciences
catalog: LS-C277167
domestic rabbit polyclonal
reactivity:
human
conjugate: biotin
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1188, PE) LS-C277168
LifeSpan Biosciences
catalog: LS-C277168
domestic rabbit polyclonal
reactivity:
human
conjugate: PE
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa25-55, APC) LS-C277169
LifeSpan Biosciences
catalog: LS-C277169
domestic rabbit polyclonal
reactivity:
human
conjugate: APC
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa25-55, FITC) LS-C277170
LifeSpan Biosciences
catalog: LS-C277170
domestic rabbit polyclonal
reactivity:
human
conjugate: FITC
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa25-55, Azide-free, HRP) LS-C277171
LifeSpan Biosciences
catalog: LS-C277171
domestic rabbit polyclonal
reactivity:
human
conjugate: HRP
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa25-55, PE) LS-C277172
LifeSpan Biosciences
catalog: LS-C277172
domestic rabbit polyclonal
reactivity:
human
conjugate: PE
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1276-1308, APC) LS-C277173
LifeSpan Biosciences
catalog: LS-C277173
domestic rabbit polyclonal
reactivity:
human
conjugate: APC
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1276-1308, AP) LS-C277174
LifeSpan Biosciences
catalog: LS-C277174
domestic rabbit polyclonal
reactivity:
human
conjugate: AP
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1276-1308, Biotin) LS-C277175
LifeSpan Biosciences
catalog: LS-C277175
domestic rabbit polyclonal
reactivity:
human
conjugate: biotin
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1276-1308, Azide-free, HRP) LS-C277176
LifeSpan Biosciences
catalog: LS-C277176
domestic rabbit polyclonal
reactivity:
human
conjugate: HRP
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1276-1308, FITC) LS-C277177
LifeSpan Biosciences
catalog: LS-C277177
domestic rabbit polyclonal
reactivity:
human
conjugate: FITC
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1276-1308, PE) LS-C277178
LifeSpan Biosciences
catalog: LS-C277178
domestic rabbit polyclonal
reactivity:
human
conjugate: PE
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1100-1150) LS-C287543
LifeSpan Biosciences
catalog: LS-C287543
domestic rabbit polyclonal
reactivity:
human
application:
immunoprecipitation
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa35-51) LS-C313024
LifeSpan Biosciences
catalog: LS-C313024
domestic rabbit polyclonal
reactivity:
human
application:
western blot
WB of ERBB4 / HER4 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant human ERRB4, 40.6KD. (162aa tag+M1-P200). Lane 1: Recombinant Human ERRB4 Protein 10ng. Lane 2: Recombinant Human ERRB4 Protein 5ng. Lane 3: Recombinant Human ERRB4 Protein 2.5ng. Lane 4: Recombinant Human ERRB4 Protein 1.25ng.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1284) LS-C192549
LifeSpan Biosciences
catalog: LS-C192549
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunocytochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1162) LS-C327307
LifeSpan Biosciences
catalog: LS-C327307
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1188) LS-C327309
LifeSpan Biosciences
catalog: LS-C327309
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1276-1308) LS-C327310
LifeSpan Biosciences
catalog: LS-C327310
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa25-55) LS-C327311
LifeSpan Biosciences
catalog: LS-C327311
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1141-1166) LS-C329625
LifeSpan Biosciences
catalog: LS-C329625
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Tyr1284) LS-C335737
LifeSpan Biosciences
catalog: LS-C335737
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Western blot analysis of extracts of A431 cells.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa992-1308, clone OTI1D9) LS-C339247
LifeSpan Biosciences
catalog: LS-C339247
mouse monoclonal (OTI1D9)
reactivity:
human
application:
western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB4.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa992-1308, clone OTI3A10) LS-C339405
LifeSpan Biosciences
catalog: LS-C339405
mouse monoclonal (OTI3A10)
reactivity:
human
application:
western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB4.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (clone OTI1E3) LS-C339407
LifeSpan Biosciences
catalog: LS-C339407
mouse monoclonal (OTI1E3)
reactivity:
human
application:
western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB4.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (phospho-Y1284) LS-C356020
LifeSpan Biosciences
catalog: LS-C356020
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Western blot analysis of HER4 (pY1284) expression in MCF7 (A); A431 EGF-treated (B) whole cell lysates.
Immunohistochemical analysis of HER4 (pY1284) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of HER4 (pY1284) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (C-Terminus) LS-C356027
LifeSpan Biosciences
catalog: LS-C356027
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Western blot analysis of HER4 expression in Y79 (A); MOLT4 (B) whole cell lysates.
Immunohistochemical analysis of HER4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of HER4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa1165-1199) LS-C356457
LifeSpan Biosciences
catalog: LS-C356457
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Anti-ERBB4 Antibody at 1:500 dilution + rat brain lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 147 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (aa29-278) LS-C372932
LifeSpan Biosciences
catalog: LS-C372932
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Western blot of ERBB4 / HER4 antibody.
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (1220-1300 aa) LS-C381531
LifeSpan Biosciences
catalog: LS-C381531
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
ELISA
,
immunohistochemistry
,
immunocytochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody (1220-1300 aa) LS-C383417
LifeSpan Biosciences
catalog: LS-C383417
domestic rabbit polyclonal
reactivity:
human
application:
ELISA
,
immunohistochemistry
,
immunocytochemistry
quantity:
price:
to the supplier
ERBB4 / HER4 Antibody LS-C400654
LifeSpan Biosciences
catalog: LS-C400654
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
Western blot analysis of HeLa cell, using ERBB4 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Human breast cancer using ERBB4 Polyclonal Antibody at dilution of 1:40.
quantity:
price:
to the supplier