LifeSpan Biosciences erbB3 antibody

PathPlus™ ERBB3 / HER3 Antibody (aa1300-C-term) LS-B2126
LifeSpan Biosciences
catalog: LS-B2126

domestic goat polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

ERBB3 / HER3 antibody (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

Anti-HER3 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

ERBB3 / HER3 antibody flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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ERBB3 / HER3 Antibody (C-Terminus, clone 11A4) LS-C4246
LifeSpan Biosciences
catalog: LS-C4246

mouse monoclonal (11A4)
reactivity: human
application: western blot

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ERBB3 / HER3 Antibody LS-C4249
LifeSpan Biosciences
catalog: LS-C4249

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation

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ERBB3 / HER3 Antibody LS-C4250
LifeSpan Biosciences
catalog: LS-C4250

mouse monoclonal
reactivity: human
application: ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

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ERBB3 / HER3 Antibody (Azide-free) LS-C4251
LifeSpan Biosciences
catalog: LS-C4251

mouse monoclonal
reactivity: human, rat
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody (Azide-free) LS-C4252
LifeSpan Biosciences
catalog: LS-C4252

mouse monoclonal
reactivity: human, rat
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody (aa1295-1323) LS-C6678
LifeSpan Biosciences
catalog: LS-C6678

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

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ERBB3 / HER3 Antibody (clone H3.90.6, Azide-free) LS-C6680
LifeSpan Biosciences
catalog: LS-C6680

mouse monoclonal (H3.90.6)
reactivity: human
application: immunocytochemistry, immunoprecipitation, flow cytometry

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ERBB3 / HER3 Antibody (C-Terminus) LS-C6691
LifeSpan Biosciences
catalog: LS-C6691

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody LS-C18797
LifeSpan Biosciences
catalog: LS-C18797

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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ERBB3 / HER3 Antibody (phospho-Tyr1222) LS-C35220
LifeSpan Biosciences
catalog: LS-C35220

domestic rabbit monoclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

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ERBB3 / HER3 Antibody (clone 2F12) LS-C87931
LifeSpan Biosciences
catalog: LS-C87931

mouse monoclonal (2F12)
reactivity: human, rat
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody (clone 2F12, Azide-free) LS-C87932
LifeSpan Biosciences
catalog: LS-C87932

mouse monoclonal (2F12)
reactivity: human, rat
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody (clone H3.90.6) LS-C87994
LifeSpan Biosciences
catalog: LS-C87994

mouse monoclonal (H3.90.6)
reactivity: human
application: immunocytochemistry, immunoprecipitation, flow cytometry

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ERBB3 / HER3 Antibody (clone H3.90.6, Biotin) LS-C87995
LifeSpan Biosciences
catalog: LS-C87995

mouse monoclonal (H3.90.6)
reactivity: human
conjugate: biotin
application: immunocytochemistry, immunoprecipitation, flow cytometry

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ERBB3 / HER3 Antibody (clone H3.105.5) LS-C88048
LifeSpan Biosciences
catalog: LS-C88048

mouse monoclonal (H3.105.5)
reactivity: human
application: immunocytochemistry, immunoprecipitation, flow cytometry

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ERBB3 / HER3 Antibody (clone 2B5) LS-C88059
LifeSpan Biosciences
catalog: LS-C88059

mouse monoclonal (2B5)
reactivity: human, rat
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody (clone 2B5, Biotin) LS-C88060
LifeSpan Biosciences
catalog: LS-C88060

mouse monoclonal (2B5)
reactivity: human, rat
conjugate: biotin
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody (clone 2B5, Azide-free) LS-C88061
LifeSpan Biosciences
catalog: LS-C88061

mouse monoclonal (2B5)
reactivity: human, rat
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody (clone 2C3) LS-C88065
LifeSpan Biosciences
catalog: LS-C88065

mouse monoclonal (2C3)
reactivity: human, rat
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody (clone 2C3, Azide-free) LS-C88066
LifeSpan Biosciences
catalog: LS-C88066

mouse monoclonal (2C3)
reactivity: human, rat
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody LS-C90417
LifeSpan Biosciences
catalog: LS-C90417

mouse monoclonal
reactivity: human
application: immunohistochemistry, flow cytometry

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ERBB3 / HER3 Antibody (aa24-55) LS-C97504
LifeSpan Biosciences
catalog: LS-C97504

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of anti-ErbB3 antibody in mouse brain lysate. ErbB3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of 293 cells using ErbB3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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ERBB3 / HER3 Antibody (clone 2B11D11) LS-C108867
LifeSpan Biosciences
catalog: LS-C108867

mouse monoclonal (2B11D11)
reactivity: human
application: western blot, ELISA

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ERBB3 / HER3 Antibody (clone 2F9) LS-C109093
LifeSpan Biosciences
catalog: LS-C109093

mouse monoclonal (2F9)
reactivity: human
application: western blot, ELISA, immunocytochemistry

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IHC-plus™ ERBB3 / HER3 Antibody (clone 3F10F6) LS-B3896
LifeSpan Biosciences
catalog: LS-B3896

mouse monoclonal (3F10F6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-HER3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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ERBB3 / HER3 Antibody (aa1191-1240) LS-C117909
LifeSpan Biosciences
catalog: LS-C117909

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HUVEC cells, using HER3 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HER3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using HER3 Antibody. The lane on the right is blocked with the synthesized peptide.
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ERBB3 / HER3 Antibody (aa1256-1305) LS-C117910
LifeSpan Biosciences
catalog: LS-C117910

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of A549 cells, using HER3 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HER3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with LiCl 0.01U/ml 15', using HER3 Antibody. The lane on the right is blocked with the synthesized peptide.
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ERBB3 / HER3 Antibody (aa1-50) LS-C118409
LifeSpan Biosciences
catalog: LS-C118409

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HER3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205 cells, using HER3 Antibody. The lane on the right is blocked with the synthesized peptide.
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ERBB3 / HER3 Antibody LS-C128812
LifeSpan Biosciences
catalog: LS-C128812

domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry

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ERBB3 / HER3 Antibody (clone 2A4) LS-C133887
LifeSpan Biosciences
catalog: LS-C133887

mouse monoclonal (2A4)
reactivity: human
application: ELISA

Detection limit for recombinant GST tagged ERBB3 is approximately 0.3 ng/ml as a capture antibody.
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ERBB3 / HER3 Antibody (clone 4H3) LS-C133889
LifeSpan Biosciences
catalog: LS-C133889

mouse monoclonal (4H3)
reactivity: human
application: ELISA

Detection limit for recombinant GST tagged ERBB3 is approximately 0.1 ng/ml as a capture antibody.
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IHC-plus™ ERBB3 / HER3 Antibody (clone 2E9) LS-B6035
LifeSpan Biosciences
catalog: LS-B6035

mouse monoclonal (2E9)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

ERBB3 monoclonal antibody, clone 2E9 Western blot of ERBB3 expression in HeLa NE.

Detection limit for recombinant GST tagged ERBB3 is 3 ng/ml as a capture antibody.

Anti-HER3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ ERBB3 / HER3 Antibody (clone 3F12) LS-B6036
LifeSpan Biosciences
catalog: LS-B6036

mouse monoclonal (3F12)
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Detection limit for recombinant GST tagged ERBB3 is approximately 3 ng/ml as a capture antibody.

IHC-plus™ ERBB3 / HER3 Antibody (clone 2C7) LS-B6037
LifeSpan Biosciences
catalog: LS-B6037

mouse monoclonal (2C7)
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Detection limit for recombinant GST tagged ERBB3 is approximately 0.3 ng/ml as a capture antibody.

Anti-HER3 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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ERBB3 / HER3 Antibody (Asp1324) LS-C177110
LifeSpan Biosciences
catalog: LS-C177110

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of HER3 (D1324) pAb in extracts from HeLa cells.
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ERBB3 / HER3 Antibody (Tyr1328) LS-C189377
LifeSpan Biosciences
catalog: LS-C189377

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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ERBB3 / HER3 Antibody (aa1293-1342) LS-C199051
LifeSpan Biosciences
catalog: LS-C199051

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HeLa cells, using HER3 Antibody. The lane on the right is blocked with the synthesized peptide.
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ERBB3 / HER3 Antibody (phospho-Tyr1197) LS-C199052
LifeSpan Biosciences
catalog: LS-C199052

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HepG2 cells , using HER3 (Phospho-Tyr1197) Antibody. The lane on the right is blocked with the phospho peptide.
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ERBB3 / HER3 Antibody (phospho-Tyr1222) LS-C199053
LifeSpan Biosciences
catalog: LS-C199053

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HUVEC cells, using HER3 (Phospho-Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using HER3 (Phospho-Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using HER3 (Phospho-Tyr1222) Antibody. The lane on the right is blocked with the phospho peptide.
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ERBB3 / HER3 Antibody (phospho-Tyr1289) LS-C199054
LifeSpan Biosciences
catalog: LS-C199054

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunocytochemistry

Immunofluorescence analysis of HeLa cells, using HER3 (Phospho-Tyr1289) Antibody. The picture on the right is blocked with the phospho peptide.
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ERBB3 / HER3 Antibody (N-Terminus) LS-C201209
LifeSpan Biosciences
catalog: LS-C201209

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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ERBB3 / HER3 Antibody (aa24-55, AP) LS-C219086
LifeSpan Biosciences
catalog: LS-C219086

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry

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ERBB3 / HER3 Antibody (aa24-55, Biotin) LS-C219087
LifeSpan Biosciences
catalog: LS-C219087

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry

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ERBB3 / HER3 Antibody (aa24-55, APC) LS-C219088
LifeSpan Biosciences
catalog: LS-C219088

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry

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ERBB3 / HER3 Antibody (aa24-55, FITC) LS-C219089
LifeSpan Biosciences
catalog: LS-C219089

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry

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ERBB3 / HER3 Antibody (aa24-55, Azide-free, HRP) LS-C219090
LifeSpan Biosciences
catalog: LS-C219090

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, flow cytometry

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ERBB3 / HER3 Antibody (aa24-55, PE) LS-C219091
LifeSpan Biosciences
catalog: LS-C219091

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, flow cytometry

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ERBB3 / HER3 Antibody (aa1275-1325) LS-C291013
LifeSpan Biosciences
catalog: LS-C291013

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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ERBB3 / HER3 Antibody (aa1292-1342) LS-C291014
LifeSpan Biosciences
catalog: LS-C291014

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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ERBB3 / HER3 Antibody (phospho-Tyr1328) LS-C291162
LifeSpan Biosciences
catalog: LS-C291162

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

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ERBB3 / HER3 Antibody (aa1275-1324) LS-C292053
LifeSpan Biosciences
catalog: LS-C292053

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from K562 cells using HER3 antibody.
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ERBB3 / HER3 Antibody LS-C310275
LifeSpan Biosciences
catalog: LS-C310275

domestic rabbit polyclonal
reactivity: human
application: western blot

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ERBB3 / HER3 Antibody (aa162-181) LS-C313025
LifeSpan Biosciences
catalog: LS-C313025

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of HER3 / ERBB3 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human ERBB3, 45.0KD. (162aa tag+ M1-A245). Lane 1: Recombinant Human ERBB3 Protein 5ng. Lane 2: Recombinant Human ERBB3 Protein 2.5ng. Lane 3: Recombinant Human ERBB3 Protein 1.25ng.
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ERBB3 / HER3 Antibody (aa24-55) LS-C327308
LifeSpan Biosciences
catalog: LS-C327308

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry

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ERBB3 / HER3 Antibody LS-C331164
LifeSpan Biosciences
catalog: LS-C331164

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cell lines, using ERBB3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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ERBB3 / HER3 Antibody LS-C334132
LifeSpan Biosciences
catalog: LS-C334132

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of A431 cells.
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ERBB3 / HER3 Antibody (phospho-Tyr1328) LS-C335936
LifeSpan Biosciences
catalog: LS-C335936

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts from A431 cells.
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ERBB3 / HER3 Antibody (clone OTI3E10) LS-C338846
LifeSpan Biosciences
catalog: LS-C338846

mouse monoclonal (OTI3E10)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Human colon tissue using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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ERBB3 / HER3 Antibody (clone OTI1C6) LS-C338847
LifeSpan Biosciences
catalog: LS-C338847

mouse monoclonal (OTI1C6)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Human lung tissue using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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ERBB3 / HER3 Antibody (clone OTI1B11) LS-C338859
LifeSpan Biosciences
catalog: LS-C338859

mouse monoclonal (OTI1B11)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB3.
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ERBB3 / HER3 Antibody (clone OTI1A9) LS-C338897
LifeSpan Biosciences
catalog: LS-C338897

mouse monoclonal (OTI1A9)
reactivity: human
application: western blot

ERBB3 / HER3 Antibody (clone OTI1A5) LS-C338899
LifeSpan Biosciences
catalog: LS-C338899

mouse monoclonal (OTI1A5)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ERBB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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ERBB3 / HER3 Antibody (clone OTI2F11) LS-C340648
LifeSpan Biosciences
catalog: LS-C340648

mouse monoclonal (OTI2F11)
reactivity: human
application: western blot

ERBB3 / HER3 Antibody (clone RBT-HER3, Ready-to-Use) LS-C348485
LifeSpan Biosciences
catalog: LS-C348485

domestic rabbit recombinant (RBT-HER3)
reactivity: human
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of HER-3 on FFPE Colon Tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.
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ERBB3 / HER3 Antibody (clone RBT-HER3) LS-C348486
LifeSpan Biosciences
catalog: LS-C348486

domestic rabbit recombinant (RBT-HER3)
reactivity: human
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of HER-3 on FFPE Colon Tissue.
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ERBB3 / HER3 Antibody (C-Terminus) LS-C356018
LifeSpan Biosciences
catalog: LS-C356018

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of HER3 (pY1328) expression in A431 EGF-treated (A); Raw264.7 (B) whole cell lysates.

Immunohistochemical analysis of HER3 (pY1328) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of HER3 (pY1328) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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ERBB3 / HER3 Antibody (aa1076-1110) LS-C356691
LifeSpan Biosciences
catalog: LS-C356691

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Anti-Erbb3 Antibody (P1076)at 1:2000 dilution + mouse lung lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 148 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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ERBB3 / HER3 Antibody (N-Terminus) LS-C368595
LifeSpan Biosciences
catalog: LS-C368595

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of HER3 expression in K562 (A); NIH3T3 (B); mouse heart (C); mouse brain (D) whole cell lysates.

Immunohistochemical analysis of HER3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of HER3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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ERBB3 / HER3 Antibody (C-Terminus) LS-C368654
LifeSpan Biosciences
catalog: LS-C368654

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of HER3 (pY1222) expression in A431 EGF-treated (A); MCF7 EGF-treated (B) whole cell lysates.
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ERBB3 / HER3 Antibody (C-Terminus) LS-C368814
LifeSpan Biosciences
catalog: LS-C368814

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of HER3 (pY1289) expression in MCF7 (A); K562 (B) whole cell lysates.
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ERBB3 / HER3 Antibody (C-Terminus) LS-C368839
LifeSpan Biosciences
catalog: LS-C368839

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of HER3 expression in HUVEC (A); HEK293T (B) whole cell lysates.
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ERBB3 / HER3 Antibody (aa709-966) LS-C372929
LifeSpan Biosciences
catalog: LS-C372929

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of ERBB3 / HER3 antibody.

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ERBB3 / HER3 Antibody (aa707-964) LS-C372931
LifeSpan Biosciences
catalog: LS-C372931

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of ERBB3 / HER3 antibody.

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ERBB3 / HER3 Antibody (1140-1220 aa) LS-C381091
LifeSpan Biosciences
catalog: LS-C381091

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot of Phospho-ErbB-3 (Y1197) antibody
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ERBB3 / HER3 Antibody (1160-1240 aa) LS-C381525
LifeSpan Biosciences
catalog: LS-C381525

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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ERBB3 / HER3 Antibody (1270-1350 aa) LS-C381580
LifeSpan Biosciences
catalog: LS-C381580

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

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ERBB3 / HER3 Antibody (1230-1310 aa) LS-C381776
LifeSpan Biosciences
catalog: LS-C381776

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Phospho-ErbB-3 (Y1289) antibody
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price:
to the supplier

ERBB3 / HER3 Antibody (1160-1240 aa) LS-C383406
LifeSpan Biosciences
catalog: LS-C383406

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of ErbB-3 antibody
quantity:
price:
to the supplier

ERBB3 / HER3 Antibody (1230-1310 aa) LS-C383411
LifeSpan Biosciences
catalog: LS-C383411

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of ErbB-3 antibody
quantity:
price:
to the supplier

ERBB3 / HER3 Antibody (1270-1350 aa) LS-C383412
LifeSpan Biosciences
catalog: LS-C383412

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of ErbB-3 antibody
quantity:
price:
to the supplier

ERBB3 / HER3 Antibody (1140-1220 aa) LS-C383414
LifeSpan Biosciences
catalog: LS-C383414

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of ErbB-3 antibody
quantity:
price:
to the supplier

ERBB3 / HER3 Antibody (1-80 aa, N-terminal) LS-C383416
LifeSpan Biosciences
catalog: LS-C383416

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of ErbB-3 antibody
quantity:
price:
to the supplier