ENO1 / Alpha Enolase Antibody (N-Terminus) LS-C31037
LifeSpan Biosciences
catalog: LS-C31037
domestic rabbit polyclonal
reactivity: human
application: western blot
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PathPlus™ ENO1 / Alpha Enolase Antibody (clone 8G8) LS-B4318
LifeSpan Biosciences
catalog: LS-B4318
mouse monoclonal (8G8)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.

Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).

Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.
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PathPlus™ ENO1 / Alpha Enolase Antibody (aa199-434) LS-B10960
LifeSpan Biosciences
catalog: LS-B10960
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Sample (30 ug of whole cell lysate). A: A431 , B: H1299. 10% SDS PAGE. ENO1 / Alpha Enolase antibody diluted at 1:3000

Immunofluorescence of paraformaldehyde-fixed HeLa using enolase 1 antibody at 1:200 dilution.

Anti-ENO1 / Alpha Enolase antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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ENO1 / Alpha Enolase Antibody (C-Terminus) LS-C31039
LifeSpan Biosciences
catalog: LS-C31039
domestic rabbit polyclonal
reactivity: human
application: western blot
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ENO1 / Alpha Enolase Antibody LS-C27288
LifeSpan Biosciences
catalog: LS-C27288
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
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ENO1 / Alpha Enolase Antibody LS-C96066
LifeSpan Biosciences
catalog: LS-C96066
chicken polyclonal
reactivity: human
application: western blot
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IHC-plus™ ENO1 / Alpha Enolase Antibody (aa178-205) LS-B3633
LifeSpan Biosciences
catalog: LS-B3633
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

ENO1 Antibody western blot of Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35 ug/lane). The ENO1 antibody detected the ENO1 protein (arrow).

ENO1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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ENO1 / Alpha Enolase Antibody (aa44-93) LS-C135049
LifeSpan Biosciences
catalog: LS-C135049
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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ENO1 / Alpha Enolase Antibody (clone 28) LS-C137598
LifeSpan Biosciences
catalog: LS-C137598
mouse monoclonal (28)
reactivity: human
application: western blot, ELISA
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PathPlus™ ENO1 / Alpha Enolase Antibody (aa246-295) LS-B6703
LifeSpan Biosciences
catalog: LS-B6703
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ENO1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


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ENO1 / Alpha Enolase Antibody (HRP) LS-C183172
LifeSpan Biosciences
catalog: LS-C183172
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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ENO1 / Alpha Enolase Antibody (FITC) LS-C183173
LifeSpan Biosciences
catalog: LS-C183173
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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ENO1 / Alpha Enolase Antibody (Biotin) LS-C183174
LifeSpan Biosciences
catalog: LS-C183174
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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ENO1 / Alpha Enolase Antibody LS-C189979
LifeSpan Biosciences
catalog: LS-C189979
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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ENO1 / Alpha Enolase Antibody (clone 253) LS-C204514
LifeSpan Biosciences
catalog: LS-C204514
mouse monoclonal (253)
reactivity: human
application: western blot, immunocytochemistry

Blot of HeLa cell lysates probed with monoclonal antibody ENO1 / Alpha Enolase antibody. The antibody stains a single sharp band corresponding to enolase-1/alpha-enolase/NNE at about 47 kDa.

Rat 3T3 cells stained with ENO1 / Alpha Enolase antibody (green) and counterstained with chicken polyclonal antibody to vimentin (red) and DNA (blue). The ENO1 / Alpha Enolase antibody antibody reveals strong cytoplasmic staining, while the vimentin antibody reveals cytoplasmic intermediate filaments.
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ENO1 / Alpha Enolase Antibody LS-C211089
LifeSpan Biosciences
catalog: LS-C211089
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Alpha-enolase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 47.7 kDa. Observed band size: 47.7 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ENO1 / Alpha Enolase Antibody (HRP) LS-C211091
LifeSpan Biosciences
catalog: LS-C211091
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Western blot of Alpha-enolase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 47.7 kDa. Observed band size: 47.7 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ENO1 / Alpha Enolase Antibody (FITC) LS-C211093
LifeSpan Biosciences
catalog: LS-C211093
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

Western blot of Alpha-enolase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 47.7 kDa. Observed band size: 47.7 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ENO1 / Alpha Enolase Antibody (Biotin) LS-C211094
LifeSpan Biosciences
catalog: LS-C211094
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Western blot of Alpha-enolase antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 47.7 kDa. Observed band size: 47.7 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ENO1 / Alpha Enolase Antibody LS-C285499
LifeSpan Biosciences
catalog: LS-C285499
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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ENO1 / Alpha Enolase Antibody LS-C310259
LifeSpan Biosciences
catalog: LS-C310259
domestic rabbit polyclonal
reactivity: human
application: western blot
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ENO1 / Alpha Enolase Antibody (FITC) LS-C316827
LifeSpan Biosciences
catalog: LS-C316827
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA
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ENO1 / Alpha Enolase Antibody (HRP) LS-C316828
LifeSpan Biosciences
catalog: LS-C316828
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA
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ENO1 / Alpha Enolase Antibody (Biotin) LS-C318749
LifeSpan Biosciences
catalog: LS-C318749
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA
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ENO1 / Alpha Enolase Antibody LS-C192429
LifeSpan Biosciences
catalog: LS-C192429
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of ENO1 pAb in extracts from PC3, Ramos, HC1-H460, Hela, THP1 cells and mouse kidney, liver tissues.
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ENO1 / Alpha Enolase Antibody LS-C331216
LifeSpan Biosciences
catalog: LS-C331216
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of various cell lines, using ENO1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunofluorescence analysis of HeLa cells using ENO1 antibody. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody. Western blot was performed from the immunoprecipitate using ENO1 antibodyat a dilition of 1:1000.
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PathPlus™ ENO1 / Alpha Enolase Antibody (clone 9) LS-B11765
LifeSpan Biosciences
catalog: LS-B11765
mouse monoclonal (9)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

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PathPlus™ ENO1 / Alpha Enolase Antibody (clone 12) LS-B11766
LifeSpan Biosciences
catalog: LS-B11766
mouse monoclonal (12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

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PathPlus™ ENO1 / Alpha Enolase Antibody (clone 786CT6.6.4) LS-B11813
LifeSpan Biosciences
catalog: LS-B11813
mouse monoclonal (786CT6.6.4)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

ENO1 Antibody western blot of MCF-7,A431,HeLa,HepG2,mouse NIH/3T3 cell line and mouse stomach,liver tissue lysates (35 ug/lane). The ENO1 antibody detected the ENO1 protein (arrow).
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