IHC-plus™ EN2 Antibody (C-Terminus) LS-B3477
LifeSpan Biosciences
catalog: LS-B3477

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

Anti-EN2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
quantity:
price:
to the supplier

EN2 Antibody (aa320-333) LS-C54787
LifeSpan Biosciences
catalog: LS-C54787

domestic goat polyclonal
reactivity: human
application: western blot

Antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
quantity:
price:
to the supplier

EN2 Antibody (aa243-271) LS-C100662
LifeSpan Biosciences
catalog: LS-C100662

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of EN2 antibody in 293 cell line lysates and mouse stomach and lung tissue lysates (35 ug/lane). EN2 (arrow) was detected using the purified antibody.

Fluorescent confocal image of HeLa cell stained with EN2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.
quantity:
price:
to the supplier

EN2 Antibody (aa243-271) LS-C167753
LifeSpan Biosciences
catalog: LS-C167753

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of EN2 Antibody in mouse cerebellum, brain tissue lysates (35 ug/lane). EN2 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human brain tissue reacted with EN2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

EN2 Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
quantity:
price:
to the supplier

IHC-plus™ EN2 Antibody (aa243-271) LS-B9057
LifeSpan Biosciences
catalog: LS-B9057

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of EN2 antibody (C-term-2) in NCI-H460 cell line lysates (35 ug/lane). EN2 (arrow) was detected using the purified antibody.

EN2 Antibody (C-term-2) flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
quantity:
price:
to the supplier

EN2 Antibody (clone 1E1) LS-C197134
LifeSpan Biosciences
catalog: LS-C197134

mouse monoclonal (1E1)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged EN2 is 3 ng/ml as a capture antibody.
quantity:
price:
to the supplier

EN2 Antibody (aa243-271, AP) LS-C277091
LifeSpan Biosciences
catalog: LS-C277091

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, Biotin) LS-C277092
LifeSpan Biosciences
catalog: LS-C277092

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, APC) LS-C277093
LifeSpan Biosciences
catalog: LS-C277093

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, FITC) LS-C277094
LifeSpan Biosciences
catalog: LS-C277094

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, Azide-free, HRP) LS-C277095
LifeSpan Biosciences
catalog: LS-C277095

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, PE) LS-C277096
LifeSpan Biosciences
catalog: LS-C277096

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, AP) LS-C277097
LifeSpan Biosciences
catalog: LS-C277097

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, APC) LS-C277098
LifeSpan Biosciences
catalog: LS-C277098

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, Biotin) LS-C277099
LifeSpan Biosciences
catalog: LS-C277099

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, FITC) LS-C277100
LifeSpan Biosciences
catalog: LS-C277100

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, PE) LS-C277101
LifeSpan Biosciences
catalog: LS-C277101

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271, Azide-free, HRP) LS-C277102
LifeSpan Biosciences
catalog: LS-C277102

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa64-93) LS-C167754
LifeSpan Biosciences
catalog: LS-C167754

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry

Western blot analysis of EN2 antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). EN2 (arrow) was detected using the purified Pab.
quantity:
price:
to the supplier

EN2 Antibody (aa243-271) LS-C327270
LifeSpan Biosciences
catalog: LS-C327270

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

EN2 Antibody (aa243-271) LS-C327271
LifeSpan Biosciences
catalog: LS-C327271

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier