EIF4E Antibody (phospho-Ser209) LS-C9778
LifeSpan Biosciences
catalog: LS-C9778

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

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IHC-plus™ EIF4E Antibody LS-B12932
LifeSpan Biosciences
catalog: LS-B12932

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Fluorescent image of HeLa cell stained with EIF4E Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). EIF4E immunoreactivity is localized to Cytoplasm significantly.

EIF4E Antibody western blot of HeLa cell line lysates (35 ug/lane). The EIF4E antibody detected the EIF4E protein (arrow).

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
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EIF4E Antibody LS-C9776
LifeSpan Biosciences
catalog: LS-C9776

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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EIF4E Antibody (phospho-Ser209) LS-C9777
LifeSpan Biosciences
catalog: LS-C9777

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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EIF4E Antibody (aa32-61) LS-C97583
LifeSpan Biosciences
catalog: LS-C97583

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-EIF4E Antibody antibody in HL60 cell line lysates. EIF4E Antibody (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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EIF4E Antibody (phospho-Ser209) LS-C117342
LifeSpan Biosciences
catalog: LS-C117342

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eIF4E (Phospho-Ser209) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with Anisomycin 25ug/ml 30', using eIF4E (Phospho-Ser209) Antibody. The lane on the right is blocked with the phospho peptide.
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EIF4E Antibody (aa1-217) LS-C128900
LifeSpan Biosciences
catalog: LS-C128900

mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry

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EIF4E Antibody (C-Terminus) LS-C145290
LifeSpan Biosciences
catalog: LS-C145290

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

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IHC-plus™ EIF4E Antibody (C-Terminus) LS-B6817
LifeSpan Biosciences
catalog: LS-B6817

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-EIF4E antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ EIF4E Antibody (aa168-217) LS-B7007
LifeSpan Biosciences
catalog: LS-B7007

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-EIF4E antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot analysis of lysates from NIH/3T3 cells, treated with FBS, using eIF4E Antibody. The lane on the right is blocked with the synthesized peptide.
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EIF4E Antibody LS-C167701
LifeSpan Biosciences
catalog: LS-C167701

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of EIF4E Antibody in HL-60 cell line lysates (35 ug/lane). EIF4E (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human testis tissue reacted with EIF4E Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

EIF4E Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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EIF4E Antibody (clone 5D11) LS-C169339
LifeSpan Biosciences
catalog: LS-C169339

mouse monoclonal (5D11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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EIF4E Antibody (clone OTI6C3) LS-C172763
LifeSpan Biosciences
catalog: LS-C172763

mouse monoclonal (OTI6C3)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E.

IHC of paraffin-embedded Human pancreas tissue using anti-EIF4E mouse monoclonal antibody.
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EIF4E Antibody (clone OTI6C11) LS-C173004
LifeSpan Biosciences
catalog: LS-C173004

mouse monoclonal (OTI6C11)
reactivity: human
application: western blot, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E.

Flow cytometry of HeLa cells, using anti-EIF4E antibody (Red), compared to a nonspecific negative control antibody (Blue).
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EIF4E Antibody (clone OTI9H1) LS-C173020
LifeSpan Biosciences
catalog: LS-C173020

mouse monoclonal (OTI9H1)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E.
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EIF4E Antibody (clone OTI5D11) LS-C173027
LifeSpan Biosciences
catalog: LS-C173027

mouse monoclonal (OTI5D11)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E.

IHC of paraffin-embedded Human Kidney tissue using anti-EIF4E mouse monoclonal antibody.
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EIF4E Antibody (Thr203) LS-C177501
LifeSpan Biosciences
catalog: LS-C177501

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of eIF4E (T203) pAb in extracts from COLO205 cells.

IHC of eIF4E (T203) pAb in paraffin-embedded human breast carcinoma tissue.
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EIF4E Antibody (phospho-Ser209) LS-C178024
LifeSpan Biosciences
catalog: LS-C178024

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-eIF4E (S209) pAb in extracts from 293 Anisomycin cells.

IHC of p-eIF4E (S209) pAb in paraffin-embedded human brain tissue.
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EIF4E Antibody (phospho-Ser209) LS-C178084
LifeSpan Biosciences
catalog: LS-C178084

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-eIF4E (S209) pAb in extracts from NIH/3T3 FBS cells.

IHC of p-eIF4E (S209) pAb in paraffin-embedded human breast carcinoma tissue.
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EIF4E Antibody (Arg215) LS-C178169
LifeSpan Biosciences
catalog: LS-C178169

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of eIF4E (R215) pAb in extracts from NIH/3T3 cells.

IHC of eIF4E (R215) pAb in paraffin-embedded human breast carcinoma tissue.
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EIF4E Antibody (aa2-217) LS-C178544
LifeSpan Biosciences
catalog: LS-C178544

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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EIF4E Antibody (Ser209) LS-C189378
LifeSpan Biosciences
catalog: LS-C189378

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

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EIF4E Antibody (phospho-Ser209) LS-C189379
LifeSpan Biosciences
catalog: LS-C189379

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

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EIF4E Antibody (APC) LS-C213758
LifeSpan Biosciences
catalog: LS-C213758

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry

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EIF4E Antibody (Azide-free, HRP) LS-C213759
LifeSpan Biosciences
catalog: LS-C213759

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, flow cytometry

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EIF4E Antibody (Biotin) LS-C213760
LifeSpan Biosciences
catalog: LS-C213760

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry

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EIF4E Antibody (FITC) LS-C213761
LifeSpan Biosciences
catalog: LS-C213761

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry

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EIF4E Antibody (AP) LS-C213762
LifeSpan Biosciences
catalog: LS-C213762

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry

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EIF4E Antibody (PE) LS-C213767
LifeSpan Biosciences
catalog: LS-C213767

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, flow cytometry

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EIF4E Antibody (aa32-61, AP) LS-C219165
LifeSpan Biosciences
catalog: LS-C219165

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

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EIF4E Antibody (aa32-61, APC) LS-C219166
LifeSpan Biosciences
catalog: LS-C219166

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

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EIF4E Antibody (aa32-61, Biotin) LS-C219167
LifeSpan Biosciences
catalog: LS-C219167

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

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EIF4E Antibody (aa32-61, FITC) LS-C219168
LifeSpan Biosciences
catalog: LS-C219168

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

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EIF4E Antibody (aa32-61, Azide-free, HRP) LS-C219169
LifeSpan Biosciences
catalog: LS-C219169

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

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EIF4E Antibody (aa32-61, PE) LS-C219170
LifeSpan Biosciences
catalog: LS-C219170

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

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EIF4E Antibody (phospho-Ser209, AP) LS-C219171
LifeSpan Biosciences
catalog: LS-C219171

domestic rabbit polyclonal
reactivity: human
conjugate: AP
modification: phosphorylated
application: ELISA

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EIF4E Antibody (phospho-Ser209, APC) LS-C219172
LifeSpan Biosciences
catalog: LS-C219172

domestic rabbit polyclonal
reactivity: human
conjugate: APC
modification: phosphorylated
application: ELISA

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EIF4E Antibody (phospho-Ser209, Biotin) LS-C219173
LifeSpan Biosciences
catalog: LS-C219173

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
modification: phosphorylated
application: ELISA

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EIF4E Antibody (phospho-Ser209, FITC) LS-C219174
LifeSpan Biosciences
catalog: LS-C219174

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
modification: phosphorylated
application: ELISA

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EIF4E Antibody (phospho-Ser209, Azide-free, HRP) LS-C219175
LifeSpan Biosciences
catalog: LS-C219175

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
modification: phosphorylated
application: ELISA

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EIF4E Antibody (phospho-Ser209, PE) LS-C219176
LifeSpan Biosciences
catalog: LS-C219176

domestic rabbit polyclonal
reactivity: human
conjugate: PE
modification: phosphorylated
application: ELISA

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EIF4E Antibody (clone 163CT48.1.9, AP) LS-C221240
LifeSpan Biosciences
catalog: LS-C221240

mouse monoclonal (163CT48.1.9)
reactivity: human
conjugate: AP
application: western blot, ELISA, immunocytochemistry

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EIF4E Antibody (clone 163CT48.1.9, APC) LS-C221241
LifeSpan Biosciences
catalog: LS-C221241

mouse monoclonal (163CT48.1.9)
reactivity: human
conjugate: APC
application: western blot, ELISA, immunocytochemistry

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EIF4E Antibody (clone 163CT48.1.9, Biotin) LS-C221242
LifeSpan Biosciences
catalog: LS-C221242

mouse monoclonal (163CT48.1.9)
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunocytochemistry

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EIF4E Antibody (clone 163CT48.1.9, FITC) LS-C221243
LifeSpan Biosciences
catalog: LS-C221243

mouse monoclonal (163CT48.1.9)
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunocytochemistry

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EIF4E Antibody (clone 163CT48.1.9, Azide-free, HRP) LS-C221244
LifeSpan Biosciences
catalog: LS-C221244

mouse monoclonal (163CT48.1.9)
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunocytochemistry

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EIF4E Antibody (clone 163CT48.1.9, PE) LS-C221245
LifeSpan Biosciences
catalog: LS-C221245

mouse monoclonal (163CT48.1.9)
reactivity: human
conjugate: PE
application: western blot, ELISA, immunocytochemistry

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EIF4E Antibody (aa167-217) LS-C287980
LifeSpan Biosciences
catalog: LS-C287980

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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EIF4E Antibody (aa1-50) LS-C287981
LifeSpan Biosciences
catalog: LS-C287981

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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EIF4E Antibody LS-C192761
LifeSpan Biosciences
catalog: LS-C192761

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of HEK-293 cell lines, using EIF4E antibody.
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EIF4E Antibody (clone 163CT48.1.9) LS-C319147
LifeSpan Biosciences
catalog: LS-C319147

mouse monoclonal (163CT48.1.9)
reactivity: human
application: western blot, ELISA, immunocytochemistry

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EIF4E Antibody LS-C321077
LifeSpan Biosciences
catalog: LS-C321077

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry

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to the supplier

EIF4E Antibody (phospho-Ser209) LS-C327340
LifeSpan Biosciences
catalog: LS-C327340

domestic rabbit polyclonal
reactivity: human
application: ELISA

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EIF4E Antibody LS-C331949
LifeSpan Biosciences
catalog: LS-C331949

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human kidney tissue.
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EIF4E Antibody (C-Terminus) LS-C352157
LifeSpan Biosciences
catalog: LS-C352157

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of EIF4E expression in HEK293T (A); NIH3T3 (B) whole cell lysates.

Immunohistochemical analysis of EIF4E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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EIF4E Antibody (clone 1504CT774.14.55) LS-C356292
LifeSpan Biosciences
catalog: LS-C356292

mouse monoclonal (1504CT774.14.55)
reactivity: human
application: western blot

All lanes : Anti-IF4E Antibody at 1:4000 dilution Lane 1: HeLa whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: K562 whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lane 5: PC-12 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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EIF4E Antibody (140-220 aa) LS-C380690
LifeSpan Biosciences
catalog: LS-C380690

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-eIF4E (S209) antibody
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EIF4E Antibody (140-220 aa) LS-C383319
LifeSpan Biosciences
catalog: LS-C383319

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of eIF4E antibody
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