IHC-plus™ ZIF268 / EGR1 Antibody LS-B28
LifeSpan Biosciences
catalog: LS-B28

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-EGR1 antibody IHC of human lung small cell carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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ZIF268 / EGR1 Antibody (aa53-102) LS-C31244
LifeSpan Biosciences
catalog: LS-C31244

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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ZIF268 / EGR1 Antibody (clone 6E8) LS-C104940
LifeSpan Biosciences
catalog: LS-C104940

mouse monoclonal (6E8)
reactivity: human
application: western blot, ELISA, immunocytochemistry

EGR1 monoclonal antibody, clone 6E8. Western blot of EGR1 expression in Raw 264.7.

Immunofluorescence of monoclonal antibody to EGR1 on HeLa cell (antibody concentration 10 ug/ml).

Detection limit for recombinant GST tagged EGR1 is approximately 0.1 ng/ml as a capture antibody.
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ZIF268 / EGR1 Antibody (aa282-433) LS-C36221
LifeSpan Biosciences
catalog: LS-C36221

rat monoclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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ZIF268 / EGR1 Antibody (aa467-516) LS-C107931
LifeSpan Biosciences
catalog: LS-C107931

domestic rabbit polyclonal
reactivity: human
application: western blot

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ZIF268 / EGR1 Antibody (clone 8A6) LS-C109143
LifeSpan Biosciences
catalog: LS-C109143

mouse monoclonal (8A6)
reactivity: human
application: western blot, ELISA

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ZIF268 / EGR1 Antibody (aa451-500) LS-C115991
LifeSpan Biosciences
catalog: LS-C115991

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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ZIF268 / EGR1 Antibody LS-C137415
LifeSpan Biosciences
catalog: LS-C137415

domestic rabbit polyclonal
reactivity: human
application: western blot

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ZIF268 / EGR1 Antibody (aa9-37) LS-C167664
LifeSpan Biosciences
catalog: LS-C167664

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

EGR1 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The EGR1 antibody detected the EGR1 protein (arrow).

EGR1 Antibody flow cytometry of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescence of EGR1 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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ZIF268 / EGR1 Antibody (aa1-50) LS-C290068
LifeSpan Biosciences
catalog: LS-C290068

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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ZIF268 / EGR1 Antibody (aa282-433) LS-C293720
LifeSpan Biosciences
catalog: LS-C293720

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Mouse Brain Tissue.

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ZIF268 / EGR1 Antibody (aa273-431) LS-C293722
LifeSpan Biosciences
catalog: LS-C293722

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant ZIF268 / EGR1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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ZIF268 / EGR1 Antibody (FITC) LS-C317697
LifeSpan Biosciences
catalog: LS-C317697

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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ZIF268 / EGR1 Antibody (Biotin) LS-C317698
LifeSpan Biosciences
catalog: LS-C317698

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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ZIF268 / EGR1 Antibody LS-C317699
LifeSpan Biosciences
catalog: LS-C317699

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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ZIF268 / EGR1 Antibody (HRP) LS-C317701
LifeSpan Biosciences
catalog: LS-C317701

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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ZIF268 / EGR1 Antibody (C-Terminus) LS-C352150
LifeSpan Biosciences
catalog: LS-C352150

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of EGR1 expression in MCF7 (A); SHSY5Y (B); PC12 (C) whole cell lysates.

Immunohistochemical analysis of EGR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of EGR1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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