domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry
reactivity: human
application: western blot, ELISA, immunocytochemistry
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
With 293T cell line lysate,resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EF1A1(1:500, 4 degrees C overnight ) and horseradish peroxidase-conjugated second antibody (rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).
Formalin-fixed and paraffin-embedded human brain with EEF1A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric of NCI-H292 cells using EEF1A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
quantity:
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Western blot of EEF1A1 Antibody in MCF-7 cell line lysates (35 ug/lane). EEF1A1 (arrow) was detected using the purified antibody.
Flow cytometric of NCI-H292 cells using EEF1A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescence of EEF1A1 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
quantity:
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
reactivity: human
application: western blot, immunocytochemistry
Knockout Varification: Lane 1: Wild-type Jurkat cell lysate; Lane 2: EEF1a1 knockout Jurkat cell lysate; Predicted MW: 48,50kd Observed MW: 50kd Primary Ab: 1µg/ml Rabbit Anti-Human EEF1a1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
quantity:
price:
to the supplier