DHFR Antibody (aa135-164) LS-C100566
LifeSpan Biosciences
catalog: LS-C100566
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of DHFR (arrow) using rabbit polyclonal DHFR Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHFR gene.

DHFR Antibody IHC of formalin-fixed and paraffin-embedded human lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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DHFR Antibody (aa1-187) LS-C128641
LifeSpan Biosciences
catalog: LS-C128641
domestic rabbit polyclonal
reactivity: human
application: western blot
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DHFR Antibody (clone OTI3A11) LS-C114614
LifeSpan Biosciences
catalog: LS-C114614
mouse monoclonal (OTI3A11)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR.

IHC of paraffin-embedded breast tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).
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DHFR Antibody (clone OTI6G7) LS-C114640
LifeSpan Biosciences
catalog: LS-C114640
mouse monoclonal (OTI6G7)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-DHFR monoclonal antibody.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).

Anti-DHFR mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DHFR.
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DHFR Antibody (clone 2B10) LS-C197047
LifeSpan Biosciences
catalog: LS-C197047
mouse monoclonal (2B10)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western Blot analysis of DHFR expression in transfected 293T cell line by DHFR monoclonal antibody (M01), clone 2B10.Lane 1: DHFR transfected lysate(21.5 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to DHFR on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged DHFR is approximately 0.1 ng/ml as a capture antibody.
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DHFR Antibody LS-C192755
LifeSpan Biosciences
catalog: LS-C192755
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of various cell lines, using DHFR antibody.
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DHFR Antibody (aa2-187) LS-C344027
LifeSpan Biosciences
catalog: LS-C344027
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

DHFR antibody Western blot. All lanes: Anti DHFR at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Mouse Liver Tissue Lysate at 50 ug. Lane 3: HepG2 Whole Cell Lysate at 40 ug. Lane 4: Jurkat Whole Cell Lysate at 40 ug. Predicted band size: 21 kD. Observed band size: 21 kD.

DHFR antibody IHC-paraffin: Mouse Brain Tissue.
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DHFR Antibody (aa16-30) LS-C344257
LifeSpan Biosciences
catalog: LS-C344257
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemical of paraffin-embedded H. liver section using DHFR Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot of lysates from 293T, HeLa, Jurkat, Y79 cell line , mouse spleen and rat kidney tissue lysate (from left to right) with DHFR Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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DHFR Antibody (aa2-187, FITC) LS-C369533
LifeSpan Biosciences
catalog: LS-C369533
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA
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DHFR Antibody (aa2-187) LS-C369534
LifeSpan Biosciences
catalog: LS-C369534
domestic rabbit polyclonal
reactivity: human
application: ELISA
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DHFR Antibody (aa2-187, HRP) LS-C369535
LifeSpan Biosciences
catalog: LS-C369535
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA
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DHFR Antibody (aa2-187, Biotin) LS-C369540
LifeSpan Biosciences
catalog: LS-C369540
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA
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