IHC-plus™ DFFA / ICAD / DFF45 Antibody LS-B3186
LifeSpan Biosciences
catalog: LS-B3186
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of DFF45 in HeLa cell lysate with DFF45 antibody at (A) 1 and (B) 2 µg/mL.

Anti-DFFA / ICAD antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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IHC-plus™ DFFA / ICAD / DFF45 Antibody LS-B558
LifeSpan Biosciences
catalog: LS-B558
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-DFFA / ICAD antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 ug/ml.
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DFFA / ICAD / DFF45 Antibody (aa151-200) LS-C119544
LifeSpan Biosciences
catalog: LS-C119544
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DFFA Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat, 293, and HeLa cells, using DFFA Antibody. The lane on the right is blocked with the synthesized peptide.
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DFFA / ICAD / DFF45 Antibody (Asp224) LS-C14839
LifeSpan Biosciences
catalog: LS-C14839
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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DFFA / ICAD / DFF45 Antibody (C-Terminus) LS-C14853
LifeSpan Biosciences
catalog: LS-C14853
domestic rabbit polyclonal
reactivity: human
application: western blot
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DFFA / ICAD / DFF45 Antibody (N-Terminus) LS-C31859
LifeSpan Biosciences
catalog: LS-C31859
domestic rabbit polyclonal
reactivity: human
application: western blot
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DFFA / ICAD / DFF45 Antibody (aa2-21) LS-C14836
LifeSpan Biosciences
catalog: LS-C14836
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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DFFA / ICAD / DFF45 Antibody (N-Terminus) LS-C48158
LifeSpan Biosciences
catalog: LS-C48158
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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DFFA / ICAD / DFF45 Antibody (aa1-331) LS-C64697
LifeSpan Biosciences
catalog: LS-C64697
domestic goat polyclonal
reactivity: human
application: western blot
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DFFA / ICAD / DFF45 Antibody LS-C68323
LifeSpan Biosciences
catalog: LS-C68323
mouse monoclonal
reactivity: human
application: western blot
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DFFA / ICAD / DFF45 Antibody (aa175-224) LS-C121037
LifeSpan Biosciences
catalog: LS-C121037
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded human lung carcinoma, using DFF45 (Cleaved-Asp224) Antibody. The picture on the right is treated with the synthesized peptide.
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DFFA / ICAD / DFF45 Antibody (aa1-331) LS-C128587
LifeSpan Biosciences
catalog: LS-C128587
mouse monoclonal
reactivity: human
application: western blot
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DFFA / ICAD / DFF45 Antibody LS-C136865
LifeSpan Biosciences
catalog: LS-C136865
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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DFFA / ICAD / DFF45 Antibody LS-C136927
LifeSpan Biosciences
catalog: LS-C136927
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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DFFA / ICAD / DFF45 Antibody LS-C153025
LifeSpan Biosciences
catalog: LS-C153025
domestic rabbit polyclonal
reactivity: human
application: western blot
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DFFA / ICAD / DFF45 Antibody (aa312-331) LS-C153030
LifeSpan Biosciences
catalog: LS-C153030
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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DFFA / ICAD / DFF45 Antibody LS-C153031
LifeSpan Biosciences
catalog: LS-C153031
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry
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DFFA / ICAD / DFF45 Antibody (aa304-331) LS-C167373
LifeSpan Biosciences
catalog: LS-C167373
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of DFFA Antibody in K562 cell line lysates (35 ug/lane). DFFA (arrow) was detected using the purified antibody.
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DFFA / ICAD / DFF45 Antibody (clone OTI5C4) LS-C114353
LifeSpan Biosciences
catalog: LS-C114353
mouse monoclonal (OTI5C4)
reactivity: human
application: western blot, immunocytochemistry

DFF45 antibody (5C4) at 1:5000 dilution + Hela cell lysate.

Anti-DFFA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.
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DFFA / ICAD / DFF45 Antibody (clone OTI3F12) LS-C114354
LifeSpan Biosciences
catalog: LS-C114354
mouse monoclonal (OTI3F12)
reactivity: human
application: western blot, immunocytochemistry

DFF45 antibody (3F12) at 1:500 dilution + Hela cell lysate.

Anti-DFFA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.
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DFFA / ICAD / DFF45 Antibody (Gln177) LS-C176879
LifeSpan Biosciences
catalog: LS-C176879
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of ICAD (Q177) pAb in extracts from Jurkat cells.

IHC of ICAD (Q177) pAb in paraffin-embedded human breast carcinoma tissue.
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DFFA / ICAD / DFF45 Antibody (clone 6B8) LS-C179767
LifeSpan Biosciences
catalog: LS-C179767
mouse monoclonal (6B8)
reactivity: human
application: western blot
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DFFA / ICAD / DFF45 Antibody (clone 2262C2a) LS-C184682
LifeSpan Biosciences
catalog: LS-C184682
mouse monoclonal (2262C2a)
reactivity: human
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DFFA / ICAD / DFF45 Antibody LS-C190091
LifeSpan Biosciences
catalog: LS-C190091
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
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DFFA / ICAD / DFF45 Antibody (clone 3A11) LS-C197044
LifeSpan Biosciences
catalog: LS-C197044
mouse monoclonal (3A11)
reactivity: human
application: western blot, ELISA

Western Blot analysis of DFFA expression in transfected 293T cell line by DFFA monoclonal antibody (M05), clone 3A11.Lane 1: DFFA transfected lysate (Predicted MW: 36.5 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged DFFA is 0.1 ng/ml as a capture antibody.
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DFFA / ICAD / DFF45 Antibody (aa304-331, AP) LS-C222073
LifeSpan Biosciences
catalog: LS-C222073
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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DFFA / ICAD / DFF45 Antibody (aa304-331, Biotin) LS-C222074
LifeSpan Biosciences
catalog: LS-C222074
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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DFFA / ICAD / DFF45 Antibody (aa304-331, APC) LS-C222075
LifeSpan Biosciences
catalog: LS-C222075
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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DFFA / ICAD / DFF45 Antibody (aa304-331, FITC) LS-C222076
LifeSpan Biosciences
catalog: LS-C222076
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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DFFA / ICAD / DFF45 Antibody (aa304-331, Azide-free, HRP) LS-C222079
LifeSpan Biosciences
catalog: LS-C222079
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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DFFA / ICAD / DFF45 Antibody (aa304-331, PE) LS-C222083
LifeSpan Biosciences
catalog: LS-C222083
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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DFFA / ICAD / DFF45 Antibody (aa271-331) LS-C286535
LifeSpan Biosciences
catalog: LS-C286535
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry
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DFFA / ICAD / DFF45 Antibody (aa271-331) LS-C288218
LifeSpan Biosciences
catalog: LS-C288218
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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DFFA / ICAD / DFF45 Antibody (C-Terminus) LS-C288219
LifeSpan Biosciences
catalog: LS-C288219
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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DFFA / ICAD / DFF45 Antibody LS-C307231
LifeSpan Biosciences
catalog: LS-C307231
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry
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DFFA / ICAD / DFF45 Antibody LS-C311091
LifeSpan Biosciences
catalog: LS-C311091
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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DFFA / ICAD / DFF45 Antibody LS-C311092
LifeSpan Biosciences
catalog: LS-C311092
domestic rabbit polyclonal
reactivity: human
application: western blot
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DFFA / ICAD / DFF45 Antibody (aa304-331) LS-C329473
LifeSpan Biosciences
catalog: LS-C329473
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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DFFA / ICAD / DFF45 Antibody LS-C330980
LifeSpan Biosciences
catalog: LS-C330980
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of HeLa cells, using DFFA antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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DFFA / ICAD / DFF45 Antibody LS-C348943
LifeSpan Biosciences
catalog: LS-C348943
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of HeLa cells using DFFA antibody. Blue: DAPI for nuclear staining.
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DFFA / ICAD / DFF45 Antibody (Internal) LS-C352123
LifeSpan Biosciences
catalog: LS-C352123
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of DFF45 expression in HeLa (A); U937 (B); WR19L (C); HEK293T (D); Jurkat (E) whole cell lysates.

Immunohistochemical analysis of DFF45 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of DFF45 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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DFFA / ICAD / DFF45 Antibody (Internal) LS-C356000
LifeSpan Biosciences
catalog: LS-C356000
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of DFF45 expression in HEK293T (A); H9C2 (B) whole cell lysates.
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DFFA / ICAD / DFF45 Antibody LS-C356882
LifeSpan Biosciences
catalog: LS-C356882
domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-DFF45 Antibody at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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DFFA / ICAD / DFF45 Antibody LS-C357931
LifeSpan Biosciences
catalog: LS-C357931
domestic rabbit polyclonal
reactivity: human
application: western blot
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DFFA / ICAD / DFF45 Antibody (aa19-262) LS-C374991
LifeSpan Biosciences
catalog: LS-C374991
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of DFFA / ICAD / DFF45 antibody.

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DFFA / ICAD / DFF45 Antibody (aa1-331) LS-C375596
LifeSpan Biosciences
catalog: LS-C375596
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: DFFA antibody at 10 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: PC-3 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 37 kDa. Observed band size: 37 kDa.

Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.
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DFFA / ICAD / DFF45 Antibody (150-230 aa, Internal) LS-C380500
LifeSpan Biosciences
catalog: LS-C380500
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of ICAD antibody
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DFFA / ICAD / DFF45 Antibody (120-200 aa, Internal) LS-C384058
LifeSpan Biosciences
catalog: LS-C384058
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of ICAD antibody
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