IHC-plus™ DDX3 / DDX3X Antibody LS-B2348
LifeSpan Biosciences
catalog: LS-B2348

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-DDX3X antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 ug/ml.

Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 ug/ml.
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DDX3 / DDX3X Antibody LS-C64576
LifeSpan Biosciences
catalog: LS-C64576

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
citations: 3

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DDX3 / DDX3X Antibody (N-Terminus) LS-C64572
LifeSpan Biosciences
catalog: LS-C64572

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ DDX3 / DDX3X Antibody LS-B2615
LifeSpan Biosciences
catalog: LS-B2615

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-DDX3X antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 ug/ml.

Western blot of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 1 and (B) 2 ug/ml.
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IHC-plus™ DDX3 / DDX3X Antibody (N-Terminus) LS-B3117
LifeSpan Biosciences
catalog: LS-B3117

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-DDX3X antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
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DDX3 / DDX3X Antibody (aa456-485) LS-C98236
LifeSpan Biosciences
catalog: LS-C98236

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of DDX3 (arrow) using rabbit polyclonal DDX3 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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DDX3 / DDX3X Antibody (N-Terminus) LS-C110145
LifeSpan Biosciences
catalog: LS-C110145

domestic rabbit polyclonal
reactivity: human
application: western blot

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DDX3 / DDX3X Antibody (aa466-515) LS-C117879
LifeSpan Biosciences
catalog: LS-C117879

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DDX3/DEAD-box Protein 3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2 cells, using DDX3/DEAD-box Protein 3 Antibody. The lane on the right is blocked with the synthesized peptide.
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DDX3 / DDX3X Antibody LS-C125439
LifeSpan Biosciences
catalog: LS-C125439

domestic rabbit polyclonal
reactivity: human
application: western blot

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DDX3 / DDX3X Antibody (Leu318) LS-C176503
LifeSpan Biosciences
catalog: LS-C176503

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of DDX3 (L318) pAb in extracts from HepG2 cells.

IHC of DDX3 (L318) pAb in paraffin-embedded human brain tissue.
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DDX3 / DDX3X Antibody (aa472-662) LS-C179108
LifeSpan Biosciences
catalog: LS-C179108

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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DDX3 / DDX3X Antibody (clone 2253C5a) LS-C184433
LifeSpan Biosciences
catalog: LS-C184433

mouse monoclonal (2253C5a)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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DDX3 / DDX3X Antibody (phospho-Thr322) LS-C198876
LifeSpan Biosciences
catalog: LS-C198876

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The picture on the right is blocked with the phospho peptide.
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DDX3 / DDX3X Antibody (aa456-485, AP) LS-C206576
LifeSpan Biosciences
catalog: LS-C206576

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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DDX3 / DDX3X Antibody (aa456-485, APC) LS-C206577
LifeSpan Biosciences
catalog: LS-C206577

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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DDX3 / DDX3X Antibody (aa456-485, Biotin) LS-C206578
LifeSpan Biosciences
catalog: LS-C206578

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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DDX3 / DDX3X Antibody (aa456-485, FITC) LS-C206579
LifeSpan Biosciences
catalog: LS-C206579

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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DDX3 / DDX3X Antibody (aa456-485, PE) LS-C206584
LifeSpan Biosciences
catalog: LS-C206584

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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DDX3 / DDX3X Antibody (aa456-485, Azide-free, HRP) LS-C207975
LifeSpan Biosciences
catalog: LS-C207975

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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DDX3 / DDX3X Antibody (aa1-50) LS-C286004
LifeSpan Biosciences
catalog: LS-C286004

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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DDX3 / DDX3X Antibody (aa1-50) LS-C286657
LifeSpan Biosciences
catalog: LS-C286657

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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DDX3 / DDX3X Antibody (aa600-C-term) LS-C287361
LifeSpan Biosciences
catalog: LS-C287361

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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DDX3 / DDX3X Antibody (aa550-600) LS-C287362
LifeSpan Biosciences
catalog: LS-C287362

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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DDX3 / DDX3X Antibody (phospho-Tyr69) LS-C291494
LifeSpan Biosciences
catalog: LS-C291494

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot analysis of lysates from HepG2 cells, using phospho-DDX3 (Phospho-Tyr69) antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DDX3 (Phospho-Tyr69) Antibody
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DDX3 / DDX3X Antibody (aa14-63) LS-C292236
LifeSpan Biosciences
catalog: LS-C292236

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysate from Jurkat cells, using DDX3 antibody.
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DDX3 / DDX3X Antibody (aa456-485) LS-C327173
LifeSpan Biosciences
catalog: LS-C327173

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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DDX3 / DDX3X Antibody LS-C334179
LifeSpan Biosciences
catalog: LS-C334179

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human breast cancer tissue.

Immunofluorescence analysis of HeLa cells.
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DDX3 / DDX3X Antibody (clone 2253C5a) LS-C342478
LifeSpan Biosciences
catalog: LS-C342478

mouse monoclonal (2253C5a)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of immunized recombinant protein, using anti-DDX3X monoclonal antibody.

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DDX3X mAb. Predicted molecular weight: 73 kDa

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX3X mAb.
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DDX3 / DDX3X Antibody (Internal) LS-C358966
LifeSpan Biosciences
catalog: LS-C358966

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of DDX3X expression in HepG2 (A); HeLa (B) whole cell lysates.

Immunohistochemical analysis of DDX3X staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of DDX3X staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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DDX3 / DDX3X Antibody (aa24-203, clone 11F11D10) LS-C359308
LifeSpan Biosciences
catalog: LS-C359308

mouse monoclonal (11F11D10)
reactivity: human
application: western blot, flow cytometry

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DDX3 / DDX3X Antibody (aa2-662, HRP) LS-C369532
LifeSpan Biosciences
catalog: LS-C369532

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Western blot. All lanes: DDX3X antibody at 12 ug/ml. Lane 1: NIH3T3 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 73 kDa. Observed band size: 73 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DDX3 / DDX3X Antibody (aa2-662, FITC) LS-C369536
LifeSpan Biosciences
catalog: LS-C369536

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

Western blot. All lanes: DDX3X antibody at 12 ug/ml. Lane 1: NIH3T3 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 73 kDa. Observed band size: 73 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DDX3 / DDX3X Antibody (aa2-662, Biotin) LS-C369537
LifeSpan Biosciences
catalog: LS-C369537

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Western blot. All lanes: DDX3X antibody at 12 ug/ml. Lane 1: NIH3T3 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 73 kDa. Observed band size: 73 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DDX3 / DDX3X Antibody (aa2-662) LS-C369547
LifeSpan Biosciences
catalog: LS-C369547

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot. All lanes: DDX3X antibody at 12 ug/ml. Lane 1: NIH3T3 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 73 kDa. Observed band size: 73 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DDX3 / DDX3X Antibody (aa1-220) LS-C375593
LifeSpan Biosciences
catalog: LS-C375593

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human cervical cancer using antibody at 1:100 dilution.
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