LifeSpan Biosciences DDIT3 antibody

IHC-plus™ DDIT3 / CHOP Antibody LS-B3652
LifeSpan Biosciences
catalog: LS-B3652

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-DDIT3 / GADD153 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa15-64) LS-C117859
LifeSpan Biosciences
catalog: LS-C117859

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HeLa cells, using CHOP Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using CHOP Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa, Jurkat, and COLO205 cells, using CHOP Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone 9C8) LS-C35922
LifeSpan Biosciences
catalog: LS-C35922

mouse monoclonal (9C8)
reactivity: human, mouse
application: western blot, immunoprecipitation

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa91-140) LS-C118458
LifeSpan Biosciences
catalog: LS-C118458

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of COS7 cells, using GADD153 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GADD153 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from LOVO cells, treated with serum 10% 15', using GADD153 Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa82-110) LS-C167321
LifeSpan Biosciences
catalog: LS-C167321

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

DDIT3 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The DDIT3 antibody detected the DDIT3 protein (arrow).

DDIT3 Antibody flow cytometry of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone OTI4G2) LS-C175756
LifeSpan Biosciences
catalog: LS-C175756

mouse monoclonal (OTI4G2)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDIT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDIT3.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone OTI4C4) LS-C175757
LifeSpan Biosciences
catalog: LS-C175757

mouse monoclonal (OTI4C4)
reactivity: human
application: western blot

DDIT3 / CHOP Antibody (Lys121) LS-C176032
LifeSpan Biosciences
catalog: LS-C176032

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of GADD153 (K121) pAb in extracts from LOVO treated with serum 10% 15' cells.

IHC of GADD153 (K121) pAb in paraffin-embedded human breast carcinoma tissue.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (Gln26) LS-C176403
LifeSpan Biosciences
catalog: LS-C176403

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of CHOP (Q26) pAb in extracts from Jurkat cells.

IHC of CHOP (Q26) pAb in paraffin-embedded human skeletal muscle tissue.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone 9C8, Biotin) LS-C180575
LifeSpan Biosciences
catalog: LS-C180575

mouse monoclonal (9C8)
reactivity: human, mouse
conjugate: biotin
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone 9C8, DY650) LS-C180576
LifeSpan Biosciences
catalog: LS-C180576

mouse monoclonal (9C8)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone 9C8, DY488) LS-C180577
LifeSpan Biosciences
catalog: LS-C180577

mouse monoclonal (9C8)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone 9C8, DY550) LS-C180579
LifeSpan Biosciences
catalog: LS-C180579

mouse monoclonal (9C8)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone 11C1019) LS-C183798
LifeSpan Biosciences
catalog: LS-C183798

domestic rabbit monoclonal (11C1019)
reactivity: human
application: western blot, immunoprecipitation

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa124) LS-C190199
LifeSpan Biosciences
catalog: LS-C190199

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone 2G3) LS-C197035
LifeSpan Biosciences
catalog: LS-C197035

mouse monoclonal (2G3)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged DDIT3 is approximately 0.03 ng/ml as a capture antibody.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (phospho-Ser30) LS-C198799
LifeSpan Biosciences
catalog: LS-C198799

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of A549 cells, using CHOP (Phospho-Ser30) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CHOP (Phospho-Ser30) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using CHOP (Phospho-Ser30) Antibody. The lane on the right is blocked with the phospho peptide.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa28-77) LS-C201241
LifeSpan Biosciences
catalog: LS-C201241

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa120-149, APC) LS-C234466
LifeSpan Biosciences
catalog: LS-C234466

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa120-149, Biotin) LS-C234467
LifeSpan Biosciences
catalog: LS-C234467

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa120-149, AP) LS-C234468
LifeSpan Biosciences
catalog: LS-C234468

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa120-149, FITC) LS-C234469
LifeSpan Biosciences
catalog: LS-C234469

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa120-149, PE) LS-C234470
LifeSpan Biosciences
catalog: LS-C234470

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa82-110, AP) LS-C234471
LifeSpan Biosciences
catalog: LS-C234471

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa82-110, APC) LS-C234472
LifeSpan Biosciences
catalog: LS-C234472

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa82-110, Biotin) LS-C234473
LifeSpan Biosciences
catalog: LS-C234473

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa82-110, FITC) LS-C234474
LifeSpan Biosciences
catalog: LS-C234474

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa82-110, PE) LS-C234479
LifeSpan Biosciences
catalog: LS-C234479

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa120-149, Azide-free, HRP) LS-C249807
LifeSpan Biosciences
catalog: LS-C249807

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa82-110, Azide-free, HRP) LS-C249808
LifeSpan Biosciences
catalog: LS-C249808

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, flow cytometry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa1-169, Biotin) LS-C299166
LifeSpan Biosciences
catalog: LS-C299166

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western blot of recombinant DDIT3 / CHOP. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
quantity:
price:
to the supplier

IHC-plus™ DDIT3 / CHOP Antibody (clone 3H7) LS-B11229
LifeSpan Biosciences
catalog: LS-B11229

mouse monoclonal (3H7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-DDIT3 / CHOP antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (clone OTI1E7) LS-C336495
LifeSpan Biosciences
catalog: LS-C336495

mouse monoclonal (OTI1E7)
reactivity: human
application: western blot

DDIT3 / CHOP Antibody (clone OTI2D3) LS-C338548
LifeSpan Biosciences
catalog: LS-C338548

mouse monoclonal (OTI2D3)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDIT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (aa120-149) LS-C167320
LifeSpan Biosciences
catalog: LS-C167320

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Fluorescent image of HeLa cell stained with DDIT3 Antibody (C-term A135). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.

DDIT3 Antibody (C-term A135) western blot of mouse testis tissue lysates (35 ug/lane). The DDIT3 antibody detected the DDIT3 protein (arrow).

DDIT3 Antibody (C-term A135) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (Internal) LS-C352113
LifeSpan Biosciences
catalog: LS-C352113

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of GADD153 expression in HeLa (A); MCF7 (B); SP2/0 (C); PC12 (D) whole cell lysates.

Immunohistochemical analysis of GADD153 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of GADD153 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (phospho-Ser30) LS-C352114
LifeSpan Biosciences
catalog: LS-C352114

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of GADD153 (pS30) expression in HeLa (A); Raw264.7 (B); rat spleen (C) whole cell lysates.
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (N-Terminus) LS-C353971
LifeSpan Biosciences
catalog: LS-C353971

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of GADD153 expression in Jurkat (A); HeLa (B) whole cell lysates.

Immunofluorescent analysis of GADD153 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier

IHC-plus™ DDIT3 / CHOP Antibody LS-B12888
LifeSpan Biosciences
catalog: LS-B12888

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of A549 cell using DDIT3 antibody. Blue: DAPI for nuclear staining.

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (10-90 aa) LS-C381506
LifeSpan Biosciences
catalog: LS-C381506

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity:
price:
to the supplier

DDIT3 / CHOP Antibody (60-140 aa, Internal) LS-C382697
LifeSpan Biosciences
catalog: LS-C382697

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of CHOP antibody
quantity:
price:
to the supplier