DCX / Doublecortin Antibody LS-C36204
LifeSpan Biosciences
catalog: LS-C36204
domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry
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DCX / Doublecortin Antibody (aa254-303) LS-C80410
LifeSpan Biosciences
catalog: LS-C80410
domestic rabbit polyclonal
reactivity: human
application: western blot
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DCX / Doublecortin Antibody (Ser128) LS-C97243
LifeSpan Biosciences
catalog: LS-C97243
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of lysate from human brain tissue lysate, using Phospho-DCX-S128. control. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DCX Antibody (S128) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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DCX / Doublecortin Antibody LS-C109402
LifeSpan Biosciences
catalog: LS-C109402
domestic rabbit polyclonal
reactivity: human
application: western blot

Sample (30 ug of whole cell lysate). A: Raji. 10 % SDS PAGE. DCX antibody diluted at 1:500
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DCX / Doublecortin Antibody (aa206-255) LS-C117747
LifeSpan Biosciences
catalog: LS-C117747
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from RAW264.7 cells, using Doublecortin Antibody. The lane on the right is blocked with the synthesized peptide.
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DCX / Doublecortin Antibody (aa232-242) LS-C139686
LifeSpan Biosciences
catalog: LS-C139686
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

DCX / Doublecortin antibody (0.01µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DCX / Doublecortin antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

DCX / Doublecortin antibody Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
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DCX / Doublecortin Antibody (aa284-297) LS-C155045
LifeSpan Biosciences
catalog: LS-C155045
domestic goat polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry

DCX antibody (0.5 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DCX / Doublecortin antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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DCX / Doublecortin Antibody (clone OTI4A3) LS-C172397
LifeSpan Biosciences
catalog: LS-C172397
mouse monoclonal (OTI4A3)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.

Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.

Flow cytometry of Jurkat cells, using anti-DCX antibody (Red), compared to a nonspecific negative control antibody (Blue).
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DCX / Doublecortin Antibody (Lys292) LS-C176506
LifeSpan Biosciences
catalog: LS-C176506
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of Doublecortin (K292) pAb in extracts from Raw264.7 cells.

IHC of Doublecortin (K292) pAb in paraffin-embedded human lung carcinoma tissue.
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IHC-plus™ DCX / Doublecortin Antibody (clone 2G5) LS-B8946
LifeSpan Biosciences
catalog: LS-B8946
mouse monoclonal (2G5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-DCX / Doublecortin antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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DCX / Doublecortin Antibody (aa362-411) LS-C80409
LifeSpan Biosciences
catalog: LS-C80409
domestic rabbit polyclonal
reactivity: human
application: western blot
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DCX / Doublecortin Antibody (clone 1G12) LS-C197029
LifeSpan Biosciences
catalog: LS-C197029
mouse monoclonal (1G12)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western Blot analysis of DCX expression in transfected 293T cell line by DCX monoclonal antibody (M01), clone 1G12.Lane 1: DCX transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to DCX on HeLa cell . [antibody concentration 10 ug/ml]
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DCX / Doublecortin Antibody (aa346-395) LS-C198889
LifeSpan Biosciences
catalog: LS-C198889
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Jurkat cells, using Doublecortin Antibody. The lane on the right is blocked with the synthesized peptide.
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DCX / Doublecortin Antibody LS-C203112
LifeSpan Biosciences
catalog: LS-C203112
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Flow cytometry of Ramos cells using Doublecortin Antibody, pAb, Rabbit shaded histogram) or with an isotype. control antibody (open histogram), followed by R-PE conjugated anti-rabbit IgG.

Immunohistochemistry of human brain tissue slide (Paraffin embedded) using Rabbit Anti-Doublecortin Polyclonal Antibody. and Purified Rabbit IgG (Whole molecule) Control (Right).

Western blot of tissue lysates using Doublecortin Antibody, pAb, Rabbit (1 ug/ml). The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 49 kD. Observed Size: 49 kD.
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DCX / Doublecortin Antibody (clone 3E1) LS-C204512
LifeSpan Biosciences
catalog: LS-C204512
mouse monoclonal (3E1)
reactivity: human
application: western blot, immunocytochemistry

Blots of crude rat brain extract from a postnatal 3 day animal stained with DCX / Doublecortin antibody. Two bands at ~45 kDa and ~35 kDa show that DCX / Doublecortin antibody binds to an epitope in the region of DCX shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A.

Rat brain neural cultures stained with DCX / Doublecortin antibody (green), chicken polyclonal antibody to GFAP (red) and DNA (blue). The DCX / Doublecortin antibody antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for DCX / Doublecortin antibody. This DCX antibody is therefore an excellent marker of developing neuronal cells.
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DCX / Doublecortin Antibody (Ser128, AP) LS-C276858
LifeSpan Biosciences
catalog: LS-C276858
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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DCX / Doublecortin Antibody (Ser128, APC) LS-C276859
LifeSpan Biosciences
catalog: LS-C276859
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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DCX / Doublecortin Antibody (Ser128, Biotin) LS-C276860
LifeSpan Biosciences
catalog: LS-C276860
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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DCX / Doublecortin Antibody (Ser128, FITC) LS-C276861
LifeSpan Biosciences
catalog: LS-C276861
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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DCX / Doublecortin Antibody (Ser128, Azide-free, HRP) LS-C276862
LifeSpan Biosciences
catalog: LS-C276862
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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DCX / Doublecortin Antibody (Ser128, PE) LS-C276863
LifeSpan Biosciences
catalog: LS-C276863
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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DCX / Doublecortin Antibody LS-C307197
LifeSpan Biosciences
catalog: LS-C307197
mouse polyclonal
reactivity: human
application: western blot
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DCX / Doublecortin Antibody (Ser128) LS-C327218
LifeSpan Biosciences
catalog: LS-C327218
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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DCX / Doublecortin Antibody LS-C330810
LifeSpan Biosciences
catalog: LS-C330810
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of mouse brain, using DCX antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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DCX / Doublecortin Antibody LS-C331284
LifeSpan Biosciences
catalog: LS-C331284
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of mouse brain, using DCX antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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DCX / Doublecortin Antibody (aa1-366) LS-C372972
LifeSpan Biosciences
catalog: LS-C372972
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of DCX / Doublecortin antibody.

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DCX / Doublecortin Antibody LS-C375569
LifeSpan Biosciences
catalog: LS-C375569
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry - paraffin section
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DCX / Doublecortin Antibody (Biotin) LS-C375571
LifeSpan Biosciences
catalog: LS-C375571
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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DCX / Doublecortin Antibody (HRP) LS-C375572
LifeSpan Biosciences
catalog: LS-C375572
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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DCX / Doublecortin Antibody (FITC) LS-C375581
LifeSpan Biosciences
catalog: LS-C375581
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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DCX / Doublecortin Antibody (180-260 aa) LS-C383201
LifeSpan Biosciences
catalog: LS-C383201
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Doublecortin antibody
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