DGKA Antibody (aa598-628) LS-C101244
LifeSpan Biosciences
catalog: LS-C101244

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-DGKA Antibody in HL60 cell line lysates (35 ug/lane). DGKA (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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DGKA Antibody (clone 2B7) LS-C133876
LifeSpan Biosciences
catalog: LS-C133876

mouse monoclonal (2B7)
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunoperoxidase of monoclonal antibody to DGKA on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Detection limit for recombinant GST tagged DGKA is 1 ng/ml as a capture antibody.
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DGKA Antibody (aa107-156) LS-C111519
LifeSpan Biosciences
catalog: LS-C111519

domestic rabbit polyclonal
reactivity: human
application: western blot

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DGKA Antibody (aa304-353) LS-C118831
LifeSpan Biosciences
catalog: LS-C118831

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Jurkat cells, using DGKA Antibody. The lane on the right is blocked with the synthesized peptide.
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DGKA Antibody (clone OTI4H10) LS-C173421
LifeSpan Biosciences
catalog: LS-C173421

mouse monoclonal (OTI4H10)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKA.
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DGKA Antibody (clone OTI6B3) LS-C173573
LifeSpan Biosciences
catalog: LS-C173573

mouse monoclonal (OTI6B3)
reactivity: human
application: western blot, immunocytochemistry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKA.

Anti-DGKA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DGKA.
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DGKA Antibody (clone OTI4A11) LS-C173602
LifeSpan Biosciences
catalog: LS-C173602

mouse monoclonal (OTI4A11)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKA.

IHC of paraffin-embedded Human pancreas tissue using anti-DGKA mouse monoclonal antibody.

Flow cytometry of Jurkat cells, using anti-DGKA antibody (Red), compared to a nonspecific negative control antibody (Blue).
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DGKA Antibody (clone OTI7B6) LS-C173603
LifeSpan Biosciences
catalog: LS-C173603

mouse monoclonal (OTI7B6)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKA.

IHC of paraffin-embedded Human breast tissue using anti-DGKA mouse monoclonal antibody.
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DGKA Antibody (clone OTI6A11) LS-C173605
LifeSpan Biosciences
catalog: LS-C173605

mouse monoclonal (OTI6A11)
reactivity: human
application: western blot, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKA.

Flow cytometry of Jurkat cells, using anti-DGKA antibody (Red), compared to a nonspecific negative control antibody (Blue).
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DGKA Antibody (clone OTI8G5) LS-C173671
LifeSpan Biosciences
catalog: LS-C173671

mouse monoclonal (OTI8G5)
reactivity: human
application: western blot, immunocytochemistry

Western blot of extracts (35 ug) from 9 different cell lines by using anti-DGKA monoclonal antibody.

Anti-DGKA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DGKA.
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DGKA Antibody (Ala340) LS-C191952
LifeSpan Biosciences
catalog: LS-C191952

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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DGKA Antibody (aa598-628, AP) LS-C225110
LifeSpan Biosciences
catalog: LS-C225110

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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DGKA Antibody (aa598-628, APC) LS-C225111
LifeSpan Biosciences
catalog: LS-C225111

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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DGKA Antibody (aa598-628, Biotin) LS-C225112
LifeSpan Biosciences
catalog: LS-C225112

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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DGKA Antibody (aa598-628, FITC) LS-C225113
LifeSpan Biosciences
catalog: LS-C225113

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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DGKA Antibody (aa598-628, Azide-free, HRP) LS-C225114
LifeSpan Biosciences
catalog: LS-C225114

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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DGKA Antibody (aa598-628, PE) LS-C225115
LifeSpan Biosciences
catalog: LS-C225115

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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DGKA Antibody LS-C307227
LifeSpan Biosciences
catalog: LS-C307227

mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry

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DGKA Antibody LS-C311089
LifeSpan Biosciences
catalog: LS-C311089

domestic rabbit polyclonal
reactivity: human
application: western blot

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DGKA Antibody (aa598-628) LS-C327187
LifeSpan Biosciences
catalog: LS-C327187

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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DGKA Antibody (clone OTI3G7) LS-C337576
LifeSpan Biosciences
catalog: LS-C337576

mouse monoclonal (OTI3G7)
reactivity: human
application: western blot, flow cytometry

Western blot of extracts (35ug) from 9 different cell lines by using anti-DGKA monoclonal antibody.
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DGKA Antibody LS-C348983
LifeSpan Biosciences
catalog: LS-C348983

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines.
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DGKA Antibody (Internal) LS-C352104
LifeSpan Biosciences
catalog: LS-C352104

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of DGK alpha expression in MDCK (A); COS7 (B) whole cell lysates.

Immunohistochemical analysis of DGK alpha staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of DGK alpha staining in MDCK cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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DGKA Antibody (280-360 aa, Internal) LS-C383128
LifeSpan Biosciences
catalog: LS-C383128

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of DGK-alpha antibody
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