IHC-plus™ CYP17 / CYP17A1 Antibody (aa358-388) LS-B14227
LifeSpan Biosciences
catalog: LS-B14227

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2

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CYP17 / CYP17A1 Antibody (aa221-270) LS-C119484
LifeSpan Biosciences
catalog: LS-C119484

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Jurkat, A549, and HeLa cells, using Cytochrome P450 17A1 Antibody. The lane on the right is blocked with the synthesized peptide.
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CYP17 / CYP17A1 Antibody LS-C155201
LifeSpan Biosciences
catalog: LS-C155201

domestic rabbit polyclonal
reactivity: human
application: western blot

Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE CYP17A1 antibody diluted at 1:10000
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IHC-plus™ CYP17 / CYP17A1 Antibody (aa134-144) LS-B8295
LifeSpan Biosciences
catalog: LS-B8295

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody (0.3 ug/ml) staining of human ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CYP17 / CYP17A1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
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CYP17 / CYP17A1 Antibody (clone OTI9H4) LS-C173230
LifeSpan Biosciences
catalog: LS-C173230

mouse monoclonal (OTI9H4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.
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CYP17 / CYP17A1 Antibody (clone OTI3F12) LS-C173251
LifeSpan Biosciences
catalog: LS-C173251

mouse monoclonal (OTI3F12)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.
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CYP17 / CYP17A1 Antibody (clone OTI3D9) LS-C173252
LifeSpan Biosciences
catalog: LS-C173252

mouse monoclonal (OTI3D9)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.
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CYP17 / CYP17A1 Antibody (Asn249) LS-C176850
LifeSpan Biosciences
catalog: LS-C176850

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of CYP17A1 (N249) pAb in extracts from Jurkat cells.

IHC of CYP17A1 (N249) pAb in paraffin-embedded human breast carcinoma tissue.
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CYP17 / CYP17A1 Antibody (Biotin) LS-C317769
LifeSpan Biosciences
catalog: LS-C317769

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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CYP17 / CYP17A1 Antibody (HRP) LS-C317770
LifeSpan Biosciences
catalog: LS-C317770

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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CYP17 / CYP17A1 Antibody (FITC) LS-C317773
LifeSpan Biosciences
catalog: LS-C317773

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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CYP17 / CYP17A1 Antibody LS-C192749
LifeSpan Biosciences
catalog: LS-C192749

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of various cell lines, using CYP17A1 antibody.
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CYP17 / CYP17A1 Antibody LS-C331434
LifeSpan Biosciences
catalog: LS-C331434

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cells.
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CYP17 / CYP17A1 Antibody (clone OTI5G10) LS-C337466
LifeSpan Biosciences
catalog: LS-C337466

mouse monoclonal (OTI5G10)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.
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CYP17 / CYP17A1 Antibody (clone OTI3F11) LS-C337474
LifeSpan Biosciences
catalog: LS-C337474

mouse monoclonal (OTI3F11)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1.

IHC of paraffin-embedded Human breast tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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CYP17 / CYP17A1 Antibody LS-C342021
LifeSpan Biosciences
catalog: LS-C342021

mouse monoclonal
reactivity: human
application: western blot

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CYP17 / CYP17A1 Antibody (Internal) LS-C352100
LifeSpan Biosciences
catalog: LS-C352100

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Cytochrome P450 17A1 expression in HeLa (A); NIH3T3 (B); H9C2 (C); SW13 (D); ES2 (E) whole cell lysates.

Immunohistochemical analysis of Cytochrome P450 17A1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Cytochrome P450 17A1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CYP17 / CYP17A1 Antibody (aa24-508, Biotin) LS-C369494
LifeSpan Biosciences
catalog: LS-C369494

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry

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CYP17 / CYP17A1 Antibody (aa24-508, HRP) LS-C369495
LifeSpan Biosciences
catalog: LS-C369495

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry

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CYP17 / CYP17A1 Antibody (aa24-508, FITC) LS-C369496
LifeSpan Biosciences
catalog: LS-C369496

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry

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CYP17 / CYP17A1 Antibody (aa24-508) LS-C372390
LifeSpan Biosciences
catalog: LS-C372390

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human adrenal gland using antibody at 1:100 dilution.
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CYP17 / CYP17A1 Antibody (aa201-507) LS-C374303
LifeSpan Biosciences
catalog: LS-C374303

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of CYP17 / CYP17A1 antibody.

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CYP17 / CYP17A1 Antibody (aa183-508) LS-C374305
LifeSpan Biosciences
catalog: LS-C374305

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of CYP17 / CYP17A1 antibody.

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CYP17 / CYP17A1 Antibody (190-270 aa, Internal) LS-C382982
LifeSpan Biosciences
catalog: LS-C382982

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of CYP17A1 antibody
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IHC-plus™ CYP17 / CYP17A1 Antibody (aa1-508) LS-B13802
LifeSpan Biosciences
catalog: LS-B13802

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
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