domestic rabbit polyclonal
application: western blot, ELISA, immunocytochemistry
application: western blot, ELISA, immunocytochemistry
Western blot of DYKDDDDK tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (DYKDDDDK-tag Antibody, pAb, Rabbit, 1 ug/ml) The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Immunofluorescence of HEK293 cells transfected with Flag-tagged CD4 protein using DYKDDDDK-tag Antibody (DYKDDDDK-tag Antibody, pAb, Rabbit, 10 ug/ml)
quantity:
price:
to the supplier
domestic rabbit polyclonal
application: western blot, ELISA
application: western blot, ELISA
domestic rabbit polyclonal
application: western blot, ELISA
application: western blot, ELISA
mouse monoclonal
application: western blot, immunocytochemistry, immunoprecipitation
application: western blot, immunocytochemistry, immunoprecipitation
Western blot analysis of over-expressed FLAG-tagged protein in 293T cell lysate.
Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation of FLAG-tagged protein from HEK293T cells transfected with vector overexpressing Flag tag; using Anti-FLAG-tag Antibody.
quantity:
price:
to the supplier