CYP2J2 Antibody (aa231-280) LS-C119500
LifeSpan Biosciences
catalog: LS-C119500

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HepG2 cells, using Cytochrome P450 2J2 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Cytochrome P450 2J2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 and COS7 cells, using Cytochrome P450 2J2 Antibody. The lane on the right is blocked with the synthesized peptide.
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CYP2J2 Antibody (aa156-183) LS-C100653
LifeSpan Biosciences
catalog: LS-C100653

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-CYP2J2 Antibody in CEM cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.

CYP2J2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
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CYP2J2 Antibody (aa335-384) LS-C135720
LifeSpan Biosciences
catalog: LS-C135720

domestic rabbit polyclonal
reactivity: human
application: western blot

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CYP2J2 Antibody (aa96-124) LS-C167228
LifeSpan Biosciences
catalog: LS-C167228

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of CYP2J2 Antibody in HepG2 cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP2J2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of HepG2 cells using CYP2J2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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CYP2J2 Antibody (clone OTI5C9) LS-C173273
LifeSpan Biosciences
catalog: LS-C173273

mouse monoclonal (OTI5C9)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2J2.

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYP2J2 mouse monoclonal antibody.
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CYP2J2 Antibody (clone OTI3A3) LS-C173274
LifeSpan Biosciences
catalog: LS-C173274

mouse monoclonal (OTI3A3)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2J2.

Anti-CYP2J2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2J2.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP2J2 antibody, and then analyzed by flow cytometry.
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CYP2J2 Antibody (clone OTI2A9) LS-C173275
LifeSpan Biosciences
catalog: LS-C173275

mouse monoclonal (OTI2A9)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2J2.

Anti-CYP2J2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2J2.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP2J2 antibody, and then analyzed by flow cytometry.
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CYP2J2 Antibody (clone OTI3A7) LS-C173289
LifeSpan Biosciences
catalog: LS-C173289

mouse monoclonal (OTI3A7)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2J2.
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CYP2J2 Antibody (clone OTI3B4) LS-C173347
LifeSpan Biosciences
catalog: LS-C173347

mouse monoclonal (OTI3B4)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2J2.

IHC of paraffin-embedded Human liver tissue using anti-CYP2J2 mouse monoclonal antibody.
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CYP2J2 Antibody (clone OTI5C5) LS-C173348
LifeSpan Biosciences
catalog: LS-C173348

mouse monoclonal (OTI5C5)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2J2.

IHC of paraffin-embedded Human liver tissue using anti-CYP2J2 mouse monoclonal antibody.
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CYP2J2 Antibody (clone 2D10) LS-C197013
LifeSpan Biosciences
catalog: LS-C197013

mouse monoclonal (2D10)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged CYP2J2 is approximately 3 ng/ml as a capture antibody.
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CYP2J2 Antibody (aa270-315) LS-C313731
LifeSpan Biosciences
catalog: LS-C313731

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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CYP2J2 Antibody (clone OTI4B9) LS-C337482
LifeSpan Biosciences
catalog: LS-C337482

mouse monoclonal (OTI4B9)
reactivity: human
application: western blot, immunocytochemistry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2J2.

Anti-CYP2J2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2J2.
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CYP2J2 Antibody (Internal) LS-C352093
LifeSpan Biosciences
catalog: LS-C352093

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Cytochrome P450 2J2 expression in HepG2 (A); A549 (B) whole cell lysates.

Immunohistochemical analysis of Cytochrome P450 2J2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Cytochrome P450 2J2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CYP2J2 Antibody LS-C356873
LifeSpan Biosciences
catalog: LS-C356873

domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-Cytochrome P450 2J2 Antibody at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: A431 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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CYP2J2 Antibody (aa328-502) LS-C375554
LifeSpan Biosciences
catalog: LS-C375554

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
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CYP2J2 Antibody (aa328-502) LS-C375557
LifeSpan Biosciences
catalog: LS-C375557

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: CYP2J2 antibody at 4 ug/ml. Lane 1: mouse skeletal muscle tissue. Lane 2: rat liver tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 58 kDa. Observed band size: 58 kDa.

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
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CYP2J2 Antibody (200-280 aa, Internal) LS-C383015
LifeSpan Biosciences
catalog: LS-C383015

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of CYP2J2 antibody
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