IHC-plus™ CSTB / Cystatin B / Stefin B Antibody (clone M1-C1) LS-B6073
LifeSpan Biosciences
catalog: LS-B6073
mouse monoclonal (M1-C1)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of CSTB expression in MCF-7 cell lysate.

Detection limit for recombinant GST tagged CSTB is approximately 1 ng/ml as a capture antibody.

Anti-CSTB / Stefin B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ CSTB / Cystatin B / Stefin B Antibody (clone M2F1) LS-B6074
LifeSpan Biosciences
catalog: LS-B6074
mouse monoclonal (M2F1)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of CSTB expression in transfected 293T cell line by CSTB antibody. Lane 1: CSTB transfected lysate (11 KDa). Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to CSTB on A-431 cell. [antibody concentration 10 ug/ml]

Anti-CSTB / Stefin B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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CSTB / Cystatin B / Stefin B Antibody LS-C48467
LifeSpan Biosciences
catalog: LS-C48467
mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry
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CSTB / Cystatin B / Stefin B Antibody (aa1-98) LS-C64158
LifeSpan Biosciences
catalog: LS-C64158
mouse monoclonal
reactivity: human
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody (C-Terminus) LS-C80765
LifeSpan Biosciences
catalog: LS-C80765
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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CSTB / Cystatin B / Stefin B Antibody (N-Terminus) LS-C80766
LifeSpan Biosciences
catalog: LS-C80766
domestic rabbit polyclonal
reactivity: human
application: western blot
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CSTB / Cystatin B / Stefin B Antibody (aa1-98, clone 2F1) LS-C93696
LifeSpan Biosciences
catalog: LS-C93696
mouse monoclonal (2F1)
reactivity: human
application: western blot, ELISA

The Cell lysates of A549 (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CSTB (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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CSTB / Cystatin B / Stefin B Antibody (aa20-69) LS-C116994
LifeSpan Biosciences
catalog: LS-C116994
domestic rabbit polyclonal
reactivity: human
application: western blot
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CSTB / Cystatin B / Stefin B Antibody (aa49-98) LS-C118581
LifeSpan Biosciences
catalog: LS-C118581
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of A549 cells, using Stefin B Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Stefin B Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using Stefin B Antibody. The lane on the right is blocked with the synthesized peptide.
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CSTB / Cystatin B / Stefin B Antibody (aa1-99, Azide-free) LS-C132265
LifeSpan Biosciences
catalog: LS-C132265
mouse monoclonal
reactivity: human
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody LS-C137602
LifeSpan Biosciences
catalog: LS-C137602
mouse monoclonal
reactivity: human
application: western blot, ELISA
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IHC-plus™ CSTB / Cystatin B / Stefin B Antibody (aa86-98) LS-B6463
LifeSpan Biosciences
catalog: LS-B6463
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-CSTB / Stefin B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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CSTB / Cystatin B / Stefin B Antibody (aa10-39) LS-C167109
LifeSpan Biosciences
catalog: LS-C167109
domestic rabbit polyclonal
reactivity: human
application: western blot

CSTB Antibody western blot of SK-BR-3 cell line lysates (35 ug/lane). The CSTB antibody detected the CSTB protein (arrow).
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CSTB / Cystatin B / Stefin B Antibody (Asn84) LS-C176225
LifeSpan Biosciences
catalog: LS-C176225
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Stefin B (N84) pAb in extracts from A549 cells.

IHC of Stefin B (N84) pAb in paraffin-embedded human breast carcinoma tissue.
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IHC-plus™ CSTB / Cystatin B / Stefin B Antibody LS-B8792
LifeSpan Biosciences
catalog: LS-B8792
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-CSTB / Cystatin B / Stefin B antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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CSTB / Cystatin B / Stefin B Antibody (Biotin) LS-C183142
LifeSpan Biosciences
catalog: LS-C183142
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody (FITC) LS-C183143
LifeSpan Biosciences
catalog: LS-C183143
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody (HRP) LS-C183144
LifeSpan Biosciences
catalog: LS-C183144
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody LS-C189971
LifeSpan Biosciences
catalog: LS-C189971
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody (aa10-39, APC) LS-C208687
LifeSpan Biosciences
catalog: LS-C208687
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody (aa10-39, AP) LS-C208688
LifeSpan Biosciences
catalog: LS-C208688
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody (aa10-39, PE) LS-C208690
LifeSpan Biosciences
catalog: LS-C208690
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody (FITC) LS-C210968
LifeSpan Biosciences
catalog: LS-C210968
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

Western blot of Cystatin-B antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 10 kDa. Observed band size: 10 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CSTB / Cystatin B / Stefin B Antibody (HRP) LS-C210969
LifeSpan Biosciences
catalog: LS-C210969
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Western blot of Cystatin-B antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 10 kDa. Observed band size: 10 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CSTB / Cystatin B / Stefin B Antibody (Biotin) LS-C210971
LifeSpan Biosciences
catalog: LS-C210971
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Western blot of Cystatin-B antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 10 kDa. Observed band size: 10 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CSTB / Cystatin B / Stefin B Antibody (aa1-98) LS-C293620
LifeSpan Biosciences
catalog: LS-C293620
domestic rabbit polyclonal
reactivity: human, bovine
application: western blot

Western blot of recombinant CSTB / Cystatin B / Stefin B. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CSTB / Cystatin B / Stefin B Antibody (aa2-98) LS-C293621
LifeSpan Biosciences
catalog: LS-C293621
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant CSTB / Cystatin B / Stefin B. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CSTB / Cystatin B / Stefin B Antibody (aa2-98) LS-C293622
LifeSpan Biosciences
catalog: LS-C293622
domestic rabbit polyclonal
reactivity: human, rat
application: western blot


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CSTB / Cystatin B / Stefin B Antibody (aa1-98) LS-C293623
LifeSpan Biosciences
catalog: LS-C293623
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Knockout Varification: Lane 1: Wild-type HepG2 cell lysate; Lane 2: CSTB knockout HepG2 cell lysate; Predicted MW: 11kDa Observed MW: 14kDa Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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CSTB / Cystatin B / Stefin B Antibody (aa2-98, Biotin) LS-C299167
LifeSpan Biosciences
catalog: LS-C299167
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot

Western blot of recombinant CSTB / Cystatin B / Stefin B. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CSTB / Cystatin B / Stefin B Antibody (aa2-98, Biotin) LS-C299168
LifeSpan Biosciences
catalog: LS-C299168
domestic rabbit polyclonal
reactivity: human, rat
conjugate: biotin
application: western blot


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CSTB / Cystatin B / Stefin B Antibody (aa1-98, Biotin) LS-C301105
LifeSpan Biosciences
catalog: LS-C301105
domestic rabbit polyclonal
reactivity: human, bovine
conjugate: biotin
application: western blot

Western blot of recombinant CSTB / Cystatin B / Stefin B. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CSTB / Cystatin B / Stefin B Antibody (aa1-98, FITC) LS-C303730
LifeSpan Biosciences
catalog: LS-C303730
domestic rabbit polyclonal
reactivity: human, bovine
conjugate: FITC
application: western blot

Western blot of recombinant CSTB / Cystatin B / Stefin B. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CSTB / Cystatin B / Stefin B Antibody LS-C310022
LifeSpan Biosciences
catalog: LS-C310022
domestic rabbit polyclonal
reactivity: human
application: western blot
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CSTB / Cystatin B / Stefin B Antibody (aa26-45) LS-C313226
LifeSpan Biosciences
catalog: LS-C313226
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

WB of CSTB / Cystatin B / Stefin B antibody. Lane 1: A549 Cell Lysate. Lane 2: A431 Cell Lysate. Lane 3: COLO320 Cell Lysate. Lane 4: HELA Cell Lysate.

CSTB / Cystatin B / Stefin B antibody. IHC(P): Human Lung Cancer Tissue.
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CSTB / Cystatin B / Stefin B Antibody (aa10-39) LS-C329435
LifeSpan Biosciences
catalog: LS-C329435
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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CSTB / Cystatin B / Stefin B Antibody LS-C332816
LifeSpan Biosciences
catalog: LS-C332816
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cells.

Immunohistochemistry of paraffin-embedded human prostate tissue.
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CSTB / Cystatin B / Stefin B Antibody (Internal) LS-C352073
LifeSpan Biosciences
catalog: LS-C352073
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Cystatin B expression in JAR (A); EOC20 (B); U87MG (C) whole cell lysates.

Immunohistochemical analysis of Cystatin B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Cystatin B staining in EOC20 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CSTB / Cystatin B / Stefin B Antibody LS-C356864
LifeSpan Biosciences
catalog: LS-C356864
domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-Cystatin B Antibody at 1:1000 dilution Lane 1: A549 whole cell lysates Lane 2: PC-12 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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CSTB / Cystatin B / Stefin B Antibody (20-100 aa, Internal) LS-C383050
LifeSpan Biosciences
catalog: LS-C383050
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Cystatin B antibody
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