CSNK1A1 / CK1 Alpha Antibody (aa309-337) LS-C100596
LifeSpan Biosciences
catalog: LS-C100596
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CK1a antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot of anti-CK1a antibody in A375 cell lysate. CK1a (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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CSNK1A1 / CK1 Alpha Antibody (aa126-175) LS-C118904
LifeSpan Biosciences
catalog: LS-C118904
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using CKI-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CKI-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, using CKI-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
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CSNK1A1 / CK1 Alpha Antibody (C-Terminus) LS-C81669
LifeSpan Biosciences
catalog: LS-C81669
domestic rabbit polyclonal
reactivity: human
application: western blot
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CSNK1A1 / CK1 Alpha Antibody LS-C77824
LifeSpan Biosciences
catalog: LS-C77824
domestic sheep polyclonal
reactivity: human
application: western blot
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CSNK1A1 / CK1 Alpha Antibody (clone AT2E2) LS-C103485
LifeSpan Biosciences
catalog: LS-C103485
mouse monoclonal (AT2E2)
reactivity: human
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (aa287-336) LS-C118022
LifeSpan Biosciences
catalog: LS-C118022
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using CKI-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HUVEC and K562 cells, using CKI-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
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CSNK1A1 / CK1 Alpha Antibody (aa1-337) LS-C127255
LifeSpan Biosciences
catalog: LS-C127255
mouse monoclonal
reactivity: human
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (aa28-77) LS-C151270
LifeSpan Biosciences
catalog: LS-C151270
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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CSNK1A1 / CK1 Alpha Antibody (Gln317) LS-C176683
LifeSpan Biosciences
catalog: LS-C176683
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Casein Kinase I (Q317) pAb in extracts from HUVEC cells.

IHC of Casein Kinase I (Q317) pAb in paraffin-embedded human colon carcinoma tissue.
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CSNK1A1 / CK1 Alpha Antibody (Phe158) LS-C176768
LifeSpan Biosciences
catalog: LS-C176768
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Casein Kinase I (F158) pAb in extracts from HeLa cells.

IHC of Casein Kinase I (F158) pAb in paraffin-embedded human lung carcinoma tissue.
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CSNK1A1 / CK1 Alpha Antibody (aa310-327) LS-C195912
LifeSpan Biosciences
catalog: LS-C195912
domestic goat polyclonal
reactivity: human
application: western blot
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CSNK1A1 / CK1 Alpha Antibody (phospho-Tyr321) LS-C198722
LifeSpan Biosciences
catalog: LS-C198722
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HUVEC cells, using Casein Kinase I alpha (Phospho-Tyr321) Antibody. The lane on the right is blocked with the phospho peptide.
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CSNK1A1 / CK1 Alpha Antibody (phospho-Tyr294) LS-C198804
LifeSpan Biosciences
catalog: LS-C198804
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using CK-1 alpha (Phospho-Tyr294) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HT29 cells treated with heat shock, using CK-1 alpha (Phospho-Tyr294) Antibody. The lane on the right is blocked with the phospho peptide.
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, AP) LS-C255535
LifeSpan Biosciences
catalog: LS-C255535
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, APC) LS-C255536
LifeSpan Biosciences
catalog: LS-C255536
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, Biotin) LS-C255537
LifeSpan Biosciences
catalog: LS-C255537
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, FITC) LS-C255538
LifeSpan Biosciences
catalog: LS-C255538
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, PE) LS-C255539
LifeSpan Biosciences
catalog: LS-C255539
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, Azide-free, HRP) LS-C255540
LifeSpan Biosciences
catalog: LS-C255540
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, AP) LS-C275926
LifeSpan Biosciences
catalog: LS-C275926
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, APC) LS-C275927
LifeSpan Biosciences
catalog: LS-C275927
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, Biotin) LS-C275928
LifeSpan Biosciences
catalog: LS-C275928
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, Azide-free, HRP) LS-C275929
LifeSpan Biosciences
catalog: LS-C275929
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, FITC) LS-C275930
LifeSpan Biosciences
catalog: LS-C275930
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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CSNK1A1 / CK1 Alpha Antibody (aa309-337, PE) LS-C275931
LifeSpan Biosciences
catalog: LS-C275931
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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IHC-plus™ CSNK1A1 / CK1 Alpha Antibody LS-B10375
LifeSpan Biosciences
catalog: LS-B10375
chicken polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of whole rat spinal cord homogenate stained with chicken casein kinase 1 alpha antibody, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~38kDa and a less prominent band at ~34kDa corresponds to other CK1 alpha isotypes.

High power image of HeLa cells stained with chicken casein kinase 1 alpha antibody (red) and our panspecific mouse monoclonal antibody to nuclear pore complexes MCA-39C7 (green). Casein kinase 1 alpha has a particulate distribution both in the nucleus and the cytoplasm. Blue is the Hoeschst DNA stain. High quality immunocytochemical images made using this antibody are shown in reference 4.

Anti-CSNK1A1 / CK1 Alpha antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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CSNK1A1 / CK1 Alpha Antibody (phospho-Tyr294) LS-C286258
LifeSpan Biosciences
catalog: LS-C286258
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry
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CSNK1A1 / CK1 Alpha Antibody (aa109-158) LS-C286282
LifeSpan Biosciences
catalog: LS-C286282
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of the lysates from HT-29 cells using CKI-alpha antibody.
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CSNK1A1 / CK1 Alpha Antibody (aa287-337) LS-C288752
LifeSpan Biosciences
catalog: LS-C288752
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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CSNK1A1 / CK1 Alpha Antibody (Biotin) LS-C317617
LifeSpan Biosciences
catalog: LS-C317617
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody LS-C317618
LifeSpan Biosciences
catalog: LS-C317618
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (HRP) LS-C317619
LifeSpan Biosciences
catalog: LS-C317619
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (FITC) LS-C318924
LifeSpan Biosciences
catalog: LS-C318924
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (aa309-337) LS-C327020
LifeSpan Biosciences
catalog: LS-C327020
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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CSNK1A1 / CK1 Alpha Antibody (aa309-337) LS-C329429
LifeSpan Biosciences
catalog: LS-C329429
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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CSNK1A1 / CK1 Alpha Antibody (phospho-Tyr321) LS-C352048
LifeSpan Biosciences
catalog: LS-C352048
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of CK1 alpha (pY321) expression in HEK293T UV-treated (A); Jurkat (B); K562 (C); mouse liver (D); H9C2 UV-treated (E) whole cell lysates.
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CSNK1A1 / CK1 Alpha Antibody (Internal) LS-C353957
LifeSpan Biosciences
catalog: LS-C353957
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of CK1 alpha expression in MCF7 (A); Jurkat (B); NIH3T3 (C); H9C2 (D) whole cell lysates.

Immunohistochemical analysis of CK1 alpha staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of CK1 alpha staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CSNK1A1 / CK1 Alpha Antibody (Internal) LS-C354242
LifeSpan Biosciences
catalog: LS-C354242
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of CK1 alpha expression in Jurkat (A); K562 (B); NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of CK1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of CK1 alpha staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CSNK1A1 / CK1 Alpha Antibody (C-Terminus) LS-C358959
LifeSpan Biosciences
catalog: LS-C358959
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of CK1 alpha (pY294) expression in Jurkat heat shock-treated (A) whole cell lysates.

Immunohistochemical analysis of CK1 alpha (pY294) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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CSNK1A1 / CK1 Alpha Antibody (260-340 aa) LS-C380991
LifeSpan Biosciences
catalog: LS-C380991
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Casein Kinase Ialpha (Y321) antibody
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CSNK1A1 / CK1 Alpha Antibody (230-310 aa) LS-C381373
LifeSpan Biosciences
catalog: LS-C381373
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Casein Kinase I alpha (Y294) antibody
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CSNK1A1 / CK1 Alpha Antibody (100-180 aa, Internal) LS-C382440
LifeSpan Biosciences
catalog: LS-C382440
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Casein Kinase I alpha antibody
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CSNK1A1 / CK1 Alpha Antibody (260-340 aa) LS-C382442
LifeSpan Biosciences
catalog: LS-C382442
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Casein Kinase Ialpha antibody
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