IHC-plus™ PARK7 / DJ-1 Antibody (C-Terminus) LS-B1452
LifeSpan Biosciences
catalog: LS-B1452
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PARK7 / DJ-1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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PARK7 / DJ-1 Antibody (C-Terminus) LS-C2549
LifeSpan Biosciences
catalog: LS-C2549
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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PARK7 / DJ-1 Antibody LS-C38081
LifeSpan Biosciences
catalog: LS-C38081
mouse monoclonal
reactivity: human
application: western blot, ELISA
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PARK7 / DJ-1 Antibody (C-Terminus) LS-C81624
LifeSpan Biosciences
catalog: LS-C81624
domestic rabbit polyclonal
reactivity: human
application: western blot
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PARK7 / DJ-1 Antibody LS-C83396
LifeSpan Biosciences
catalog: LS-C83396
domestic rabbit polyclonal
reactivity: human
application: western blot
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PARK7 / DJ-1 Antibody (clone 1B11) LS-C93700
LifeSpan Biosciences
catalog: LS-C93700
mouse monoclonal (1B11)
reactivity: human
application: western blot, ELISA

Jurkat cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Park7/DJ-1 antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.
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PARK7 / DJ-1 Antibody (aa50-100) LS-C90356
LifeSpan Biosciences
catalog: LS-C90356
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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PARK7 / DJ-1 Antibody (C-Terminus) LS-C90357
LifeSpan Biosciences
catalog: LS-C90357
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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PARK7 / DJ-1 Antibody (aa100-189) LS-C93055
LifeSpan Biosciences
catalog: LS-C93055
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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PARK7 / DJ-1 Antibody (C-Terminus) LS-C93056
LifeSpan Biosciences
catalog: LS-C93056
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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PARK7 / DJ-1 Antibody (aa1-30) LS-C99192
LifeSpan Biosciences
catalog: LS-C99192
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of anti-Park7 (DJ-1) Antibody in mouse brain,kidney,liver tissue lysates and NIH-3T3, HeLa cell line lysates (35 ug/lane). Park7 (arrow) was detected using the purified antibody.

Confocal immunofluorescence of DJ-1 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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IHC-plus™ PARK7 / DJ-1 Antibody (aa178-189) LS-B4444
LifeSpan Biosciences
catalog: LS-B4444
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-PARK7 / DJ-1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.
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PARK7 / DJ-1 Antibody (aa21-70) LS-C118430
LifeSpan Biosciences
catalog: LS-C118430
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HeLa cells, using DJ-1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using DJ-1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HUVEC cells, using DJ-1 Antibody. The lane on the right is blocked with the synthesized peptide.
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PARK7 / DJ-1 Antibody (aa61-110) LS-C119582
LifeSpan Biosciences
catalog: LS-C119582
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HUVEC, JurKat, HeLa, COLO205, and 293 cells, using PARK7 Antibody. The lane on the right is blocked with the synthesized peptide.
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PathPlus™ PARK7 / DJ-1 Antibody (aa167-189) LS-B4759
LifeSpan Biosciences
catalog: LS-B4759
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PARK7 / DJ-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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PARK7 / DJ-1 Antibody LS-C137285
LifeSpan Biosciences
catalog: LS-C137285
domestic rabbit polyclonal
reactivity: human
application: western blot
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PARK7 / DJ-1 Antibody (Pro54) LS-C175990
LifeSpan Biosciences
catalog: LS-C175990
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of DJ-1/PARK7 (P54) pAb in extracts from HUVEC cells.

IHC of DJ-1/PARK7 (P54) pAb in paraffin-embedded human lung carcinoma tissue.
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PARK7 / DJ-1 Antibody (Ala87) LS-C177137
LifeSpan Biosciences
catalog: LS-C177137
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of DJ-1 (A87) pAb in extracts from HT-29 cells.
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PARK7 / DJ-1 Antibody (Biotin) LS-C183230
LifeSpan Biosciences
catalog: LS-C183230
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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PARK7 / DJ-1 Antibody (FITC) LS-C183231
LifeSpan Biosciences
catalog: LS-C183231
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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PARK7 / DJ-1 Antibody (HRP) LS-C183232
LifeSpan Biosciences
catalog: LS-C183232
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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PARK7 / DJ-1 Antibody (aa38-87) LS-C200464
LifeSpan Biosciences
catalog: LS-C200464
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HUVECcells using PARK7 antibody.
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PARK7 / DJ-1 Antibody (aa120-149) LS-C203909
LifeSpan Biosciences
catalog: LS-C203909
domestic rabbit polyclonal
reactivity: human, zebrafish
application: western blot
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PARK7 / DJ-1 Antibody (HRP) LS-C212157
LifeSpan Biosciences
catalog: LS-C212157
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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PARK7 / DJ-1 Antibody (FITC) LS-C212159
LifeSpan Biosciences
catalog: LS-C212159
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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PARK7 / DJ-1 Antibody (Biotin) LS-C212160
LifeSpan Biosciences
catalog: LS-C212160
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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PARK7 / DJ-1 Antibody (aa140-C-term) LS-C286685
LifeSpan Biosciences
catalog: LS-C286685
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
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PARK7 / DJ-1 Antibody (aa140-C-term) LS-C287609
LifeSpan Biosciences
catalog: LS-C287609
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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PARK7 / DJ-1 Antibody (aa1-189) LS-C295769
LifeSpan Biosciences
catalog: LS-C295769
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western Blot; Sample: Human Jurkat cell lysate; Primary Ab: 2µg/ml Rabbit Anti-Human PARK7 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PARK7 / DJ-1 Antibody (aa1-189) LS-C295771
LifeSpan Biosciences
catalog: LS-C295771
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western Blot; Sample: Rat Heart lysate; Primary Ab: 3µg/ml Rabbit Anti-Mouse PARK7 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PARK7 / DJ-1 Antibody (aa1-189) LS-C295772
LifeSpan Biosciences
catalog: LS-C295772
domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western Blot; Sample: Rat Heart lysate; Primary Ab: 1µg/ml Rabbit Anti-Rat PARK7 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PARK7 / DJ-1 Antibody (aa1-189, Biotin) LS-C298081
LifeSpan Biosciences
catalog: LS-C298081
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western Blot; Sample: Human Jurkat cell lysate; Primary Ab: 2µg/ml Rabbit Anti-Human PARK7 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PARK7 / DJ-1 Antibody (aa1-189, Biotin) LS-C301111
LifeSpan Biosciences
catalog: LS-C301111
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot

Western Blot; Sample: Rat Heart lysate; Primary Ab: 3µg/ml Rabbit Anti-Mouse PARK7 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PARK7 / DJ-1 Antibody (aa1-189, Biotin) LS-C301152
LifeSpan Biosciences
catalog: LS-C301152
domestic rabbit polyclonal
reactivity: human, rat
conjugate: biotin
application: western blot

Western Blot; Sample: Rat Heart lysate; Primary Ab: 1µg/ml Rabbit Anti-Rat PARK7 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PARK7 / DJ-1 Antibody (aa1-189, FITC) LS-C302133
LifeSpan Biosciences
catalog: LS-C302133
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot

Western Blot; Sample: Rat Heart lysate; Primary Ab: 3µg/ml Rabbit Anti-Mouse PARK7 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PARK7 / DJ-1 Antibody (aa1-189, FITC) LS-C305237
LifeSpan Biosciences
catalog: LS-C305237
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot

Western Blot; Sample: Human Jurkat cell lysate; Primary Ab: 2µg/ml Rabbit Anti-Human PARK7 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PARK7 / DJ-1 Antibody (aa1-189) LS-C314329
LifeSpan Biosciences
catalog: LS-C314329
mouse monoclonal
reactivity: human, rat
application: western blot, immunocytochemistry, immunoprecipitation

Western blot of recombinant PARK7 / DJ-1.
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PARK7 / DJ-1 Antibody LS-C192312
LifeSpan Biosciences
catalog: LS-C192312
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of PARK7 pAb in extracts from Jurkat cells.
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PARK7 / DJ-1 Antibody LS-C331187
LifeSpan Biosciences
catalog: LS-C331187
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of various cell lines, using PARK7 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunofluorescence analysis of HeLa cells using PARK7 antibody. Blue: DAPI for nuclear staining.
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PARK7 / DJ-1 Antibody (aa130-164) LS-C344289
LifeSpan Biosciences
catalog: LS-C344289
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemical of paraffin-embedded H. kidney section using PARK7 Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot of lysate from HeLa cell line with PARK7 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.
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PARK7 / DJ-1 Antibody (Internal) LS-C353340
LifeSpan Biosciences
catalog: LS-C353340
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of DJ-1 expression in HeLa (A); HEK293T (B); Jurkat (C) whole cell lysates.

Immunohistochemical analysis of DJ-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of DJ-1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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PARK7 / DJ-1 Antibody (Internal) LS-C353341
LifeSpan Biosciences
catalog: LS-C353341
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of DJ-1 expression in HeLa (A); Jurkat (B); COLO205 (C); PC12 (D) whole cell lysates.

Immunohistochemical analysis of DJ-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of DJ-1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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PARK7 / DJ-1 Antibody (aa2-189) LS-C357498
LifeSpan Biosciences
catalog: LS-C357498
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

PARK7 antibody Western blot. All lanes: Anti PARK7 at 0.5 ug/ml. Lane 1: PANC Whole Cell Lysate at 40 ug. Lane 2: U20S Whole Cell Lysate at 40 ug. Lane 3: SMMC Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 20 kD. Observed band size: 20 kD.

PARK7 antibody IHC-paraffin: Rat Pancreas Tissue.

PARK7 antibody ICC: A549 Cell.
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PathPlus™ PARK7 / DJ-1 Antibody (clone 4H4) LS-B12771
LifeSpan Biosciences
catalog: LS-B12771
mouse monoclonal (4H4)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of whole HeLa cell lysates probed with PARK7 / DJ-1 antibody antibody to DJ-1. Note that the strong single band running at about 21kDa corresponds to DJ-1.

HeLa cells stained with PARK7 / DJ-1 antibody (green), and chicken antibody to vimentin (red) and DNA (blue). PARK7 / DJ-1 antibody antibody reveals strong cytoplasmic staining for DJ-1.

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PathPlus™ PARK7 / DJ-1 Antibody (clone 3E8) LS-B12808
LifeSpan Biosciences
catalog: LS-B12808
mouse monoclonal (3E8)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

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PARK7 / DJ-1 Antibody (30-110 aa, Internal) LS-C383165
LifeSpan Biosciences
catalog: LS-C383165
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of DJ-1 antibody
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PARK7 / DJ-1 Antibody (40-120 aa, Internal) LS-C385377
LifeSpan Biosciences
catalog: LS-C385377
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of PARK7 antibody
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