IHC-plus™ CHEK1 / CHK1 Antibody (aa192-241) LS-B932
LifeSpan Biosciences
catalog: LS-B932
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-Chk1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CHEK1 / CHK1 Antibody (phospho-Ser345) LS-C117319
LifeSpan Biosciences
catalog: LS-C117319
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk1 (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.
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CHEK1 / CHK1 Antibody (phospho-Ser345) LS-C6064
LifeSpan Biosciences
catalog: LS-C6064
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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to the supplier
CHEK1 / CHK1 Antibody (phospho-Ser317) LS-C6070
LifeSpan Biosciences
catalog: LS-C6070
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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price:
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CHEK1 / CHK1 Antibody LS-C26480
LifeSpan Biosciences
catalog: LS-C26480
domestic sheep polyclonal
reactivity: human
application: western blot, immunoprecipitation
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody (C-Terminus) LS-C46823
LifeSpan Biosciences
catalog: LS-C46823
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
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CHEK1 / CHK1 Antibody (N-Terminus) LS-C46830
LifeSpan Biosciences
catalog: LS-C46830
domestic rabbit polyclonal
reactivity: human, zebrafish
application: western blot, ELISA
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody LS-C46993
LifeSpan Biosciences
catalog: LS-C46993
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody (clone 2G11D5) LS-C47149
LifeSpan Biosciences
catalog: LS-C47149
mouse monoclonal (2G11D5)
reactivity: human
application: western blot, ELISA

quantity:
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CHEK1 / CHK1 Antibody LS-C60087
LifeSpan Biosciences
catalog: LS-C60087
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody (clone 2G1D5) LS-C63109
LifeSpan Biosciences
catalog: LS-C63109
mouse monoclonal (2G1D5)
reactivity: human
application: western blot

quantity:
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CHEK1 / CHK1 Antibody LS-C79127
LifeSpan Biosciences
catalog: LS-C79127
mouse monoclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody (phospho-Ser280) LS-C89007
LifeSpan Biosciences
catalog: LS-C89007
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody (phospho-Ser317) LS-C97441
LifeSpan Biosciences
catalog: LS-C97441
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Dot blot of anti-Phospho-CHK1-S317 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibodies working concentration was 0.5ug per ml.

The anti-Phospho-CHK1-S317 antibody is used in Western blot for detection in, from left to right, Jurkat, A2058, HeLa, and HL60 tissue lysates.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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CHEK1 / CHK1 Antibody (aa251-300) LS-C109689
LifeSpan Biosciences
catalog: LS-C109689
domestic rabbit polyclonal
reactivity: human
application: western blot
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CHEK1 / CHK1 Antibody (phospho-Ser280) LS-C117317
LifeSpan Biosciences
catalog: LS-C117317
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HeLa cells treated with Hu 2nM 24hours, using Chk1 (Phospho-Ser280) Antibody. The lane on the right is blocked with the phospho peptide.
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CHEK1 / CHK1 Antibody (phospho-Ser317) LS-C117318
LifeSpan Biosciences
catalog: LS-C117318
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from MCF7 cells, using Chk1 (Phospho-Ser317) Antibody. The lane on the right is blocked with the phospho peptide.
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CHEK1 / CHK1 Antibody (aa266-315) LS-C117744
LifeSpan Biosciences
catalog: LS-C117744
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Chk1 Antibody. The picture on the right is blocked with the synthesized peptide.
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CHEK1 / CHK1 Antibody (aa256-305) LS-C117857
LifeSpan Biosciences
catalog: LS-C117857
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using Chk1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COS7 and JurKat cells, using Chk1 Antibody. The lane on the right is blocked with the synthesized peptide.
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CHEK1 / CHK1 Antibody (aa271-320) LS-C117858
LifeSpan Biosciences
catalog: LS-C117858
domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry

Immunofluorescence analysis of A549 cells, using Chk1 Antibody. The picture on the right is blocked with the synthesized peptide.
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CHEK1 / CHK1 Antibody (aa286-335) LS-C118075
LifeSpan Biosciences
catalog: LS-C118075
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Chk1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, using Chk1 Antibody. The lane on the right is blocked with the synthesized peptide.
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CHEK1 / CHK1 Antibody (aa310-359) LS-C118076
LifeSpan Biosciences
catalog: LS-C118076
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HT-29 cells, treated with UV, using Chk1 Antibody. The lane on the right is blocked with the synthesized peptide.
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CHEK1 / CHK1 Antibody (phospho-Ser317) LS-C123229
LifeSpan Biosciences
catalog: LS-C123229
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
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CHEK1 / CHK1 Antibody LS-C123230
LifeSpan Biosciences
catalog: LS-C123230
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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to the supplier
CHEK1 / CHK1 Antibody LS-C123231
LifeSpan Biosciences
catalog: LS-C123231
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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price:
to the supplier
CHEK1 / CHK1 Antibody LS-C128083
LifeSpan Biosciences
catalog: LS-C128083
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody (clone 6F5) LS-C20097
LifeSpan Biosciences
catalog: LS-C20097
mouse monoclonal (6F5)
reactivity: human
application: western blot
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CHEK1 / CHK1 Antibody (Lys274) LS-C175954
LifeSpan Biosciences
catalog: LS-C175954
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of Chk1 (K274) pAb in extracts from HeLa cells.
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CHEK1 / CHK1 Antibody (Val339) LS-C175956
LifeSpan Biosciences
catalog: LS-C175956
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of Chk1 (V339) pAb in extracts from HT29 cells.
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CHEK1 / CHK1 Antibody (phospho-Ser280) LS-C177886
LifeSpan Biosciences
catalog: LS-C177886
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot of p-Chk1 (S280) pAb in extracts from HT29 cells treated with UV.
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CHEK1 / CHK1 Antibody (phospho-Ser317) LS-C177887
LifeSpan Biosciences
catalog: LS-C177887
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

Western blot of p-Chk1 (S317) pAb in extracts from MCF7 cells.
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CHEK1 / CHK1 Antibody (phospho-Ser345) LS-C177888
LifeSpan Biosciences
catalog: LS-C177888
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IHC of p-Chk1 (S345) pAb in paraffin-embedded human breast carcinoma tissue.
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CHEK1 / CHK1 Antibody (clone DCS-310) LS-C179607
LifeSpan Biosciences
catalog: LS-C179607
mouse monoclonal (DCS-310)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
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price:
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IHC-plus™ CHEK1 / CHK1 Antibody (Ser280) LS-B9014
LifeSpan Biosciences
catalog: LS-B9014
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Chk1 Antibody (S280) western blot of HeLa cell line and mouse thymus tissue lysates (35 ug/lane). The Chk1 antibody detected the Chk1 protein (arrow).

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
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CHEK1 / CHK1 Antibody (aa297-345) LS-C187106
LifeSpan Biosciences
catalog: LS-C187106
domestic rabbit polyclonal
reactivity: human
application: western blot
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CHEK1 / CHK1 Antibody (clone GF1) LS-C190278
LifeSpan Biosciences
catalog: LS-C190278
mouse monoclonal (GF1)
reactivity: human
application: ELISA
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CHEK1 / CHK1 Antibody (Ser291) LS-C193116
LifeSpan Biosciences
catalog: LS-C193116
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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CHEK1 / CHK1 Antibody (clone 2G3) LS-C196906
LifeSpan Biosciences
catalog: LS-C196906
mouse monoclonal (2G3)
reactivity: human
application: ELISA, immunocytochemistry

Immunofluorescence of monoclonal antibody to CHEK1 on HeLa cell . [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged CHEK1 is approximately 1 ng/ml as a capture antibody.
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CHEK1 / CHK1 Antibody (phospho-Ser286) LS-C198794
LifeSpan Biosciences
catalog: LS-C198794
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser286) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.
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CHEK1 / CHK1 Antibody (phospho-Ser296) LS-C198795
LifeSpan Biosciences
catalog: LS-C198795
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using Chk1 (Phospho-Ser296) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HUVEC cells treated with UV 15', using Chk1 (Phospho-Ser296) Antibody. The lane on the right is blocked with the phospho peptide.
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CHEK1 / CHK1 Antibody (phospho-Ser301) LS-C198796
LifeSpan Biosciences
catalog: LS-C198796
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells, using Chk1 (Phospho-Ser301) Antibody. The lane on the right is blocked with the phospho peptide.
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CHEK1 / CHK1 Antibody (Ser280) LS-C203036
LifeSpan Biosciences
catalog: LS-C203036
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of cell lysate using 1 ug/ml Rabbit Anti-Chk1 (Ser280) Polyclonal Antibody. Lane 1: HeLa cell lysate. Lane 2: HEK293 cell lysate. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
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CHEK1 / CHK1 Antibody (aa274-302) LS-C203902
LifeSpan Biosciences
catalog: LS-C203902
domestic rabbit polyclonal
reactivity: human
application: western blot

CHEK1 Antibody (Center S296) western blot of HepG2 cell line lysates (35 ug/lane). The CHEK1 antibody detected the CHEK1 protein (arrow).
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CHEK1 / CHK1 Antibody (aa209-220) LS-C204989
LifeSpan Biosciences
catalog: LS-C204989
domestic goat polyclonal
reactivity: human
application: western blot

CHEK1 antibody (2 ug/ml) staining of Rat Thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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CHEK1 / CHK1 Antibody (aa301-350) LS-C205628
LifeSpan Biosciences
catalog: LS-C205628
domestic rabbit polyclonal
reactivity: human
application: western blot
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CHEK1 / CHK1 Antibody (Ser280, Biotin) LS-C276205
LifeSpan Biosciences
catalog: LS-C276205
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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CHEK1 / CHK1 Antibody (Ser280, APC) LS-C276206
LifeSpan Biosciences
catalog: LS-C276206
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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CHEK1 / CHK1 Antibody (Ser280, AP) LS-C276207
LifeSpan Biosciences
catalog: LS-C276207
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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CHEK1 / CHK1 Antibody (Ser280, FITC) LS-C276208
LifeSpan Biosciences
catalog: LS-C276208
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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CHEK1 / CHK1 Antibody (Ser280, Azide-free, HRP) LS-C276209
LifeSpan Biosciences
catalog: LS-C276209
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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CHEK1 / CHK1 Antibody (Ser280, PE) LS-C276210
LifeSpan Biosciences
catalog: LS-C276210
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
quantity:
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CHEK1 / CHK1 Antibody (phospho-Ser280, AP) LS-C276211
LifeSpan Biosciences
catalog: LS-C276211
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody (phospho-Ser280, APC) LS-C276212
LifeSpan Biosciences
catalog: LS-C276212
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody (phospho-Ser280, Biotin) LS-C276213
LifeSpan Biosciences
catalog: LS-C276213
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
quantity:
price:
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CHEK1 / CHK1 Antibody (phospho-Ser280, FITC) LS-C276214
LifeSpan Biosciences
catalog: LS-C276214
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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price:
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CHEK1 / CHK1 Antibody (phospho-Ser280, Azide-free, HRP) LS-C276215
LifeSpan Biosciences
catalog: LS-C276215
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
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CHEK1 / CHK1 Antibody (phospho-Ser280, PE) LS-C276216
LifeSpan Biosciences
catalog: LS-C276216
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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CHEK1 / CHK1 Antibody (phospho-Ser317, AP) LS-C276217
LifeSpan Biosciences
catalog: LS-C276217
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
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CHEK1 / CHK1 Antibody (phospho-Ser317, APC) LS-C276218
LifeSpan Biosciences
catalog: LS-C276218
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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price:
to the supplier
CHEK1 / CHK1 Antibody (phospho-Ser317, Biotin) LS-C276219
LifeSpan Biosciences
catalog: LS-C276219
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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price:
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CHEK1 / CHK1 Antibody (phospho-Ser317, FITC) LS-C276223
LifeSpan Biosciences
catalog: LS-C276223
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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CHEK1 / CHK1 Antibody (phospho-Ser317, Azide-free, HRP) LS-C276224
LifeSpan Biosciences
catalog: LS-C276224
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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to the supplier
CHEK1 / CHK1 Antibody (phospho-Ser317, PE) LS-C276227
LifeSpan Biosciences
catalog: LS-C276227
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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IHC-plus™ CHEK1 / CHK1 Antibody LS-B10485
LifeSpan Biosciences
catalog: LS-B10485
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity:
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to the supplier
CHEK1 / CHK1 Antibody (aa250-300) LS-C285930
LifeSpan Biosciences
catalog: LS-C285930
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
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CHEK1 / CHK1 Antibody (aa200-250) LS-C287090
LifeSpan Biosciences
catalog: LS-C287090
domestic goat polyclonal
reactivity: human
application: western blot
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CHEK1 / CHK1 Antibody (aa250-300) LS-C287091
LifeSpan Biosciences
catalog: LS-C287091
domestic goat polyclonal
reactivity: human
application: western blot, immunoprecipitation
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CHEK1 / CHK1 Antibody (aa250-300) LS-C287210
LifeSpan Biosciences
catalog: LS-C287210
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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CHEK1 / CHK1 Antibody (phospho-Ser286) LS-C313575
LifeSpan Biosciences
catalog: LS-C313575
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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CHEK1 / CHK1 Antibody (clone 2G1D5) LS-C319137
LifeSpan Biosciences
catalog: LS-C319137
mouse monoclonal (2G1D5)
reactivity: human
application: western blot, ELISA
quantity:
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CHEK1 / CHK1 Antibody (phospho-Ser317) LS-C327068
LifeSpan Biosciences
catalog: LS-C327068
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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CHEK1 / CHK1 Antibody (Ser280) LS-C327070
LifeSpan Biosciences
catalog: LS-C327070
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
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CHEK1 / CHK1 Antibody (phospho-Ser280) LS-C335007
LifeSpan Biosciences
catalog: LS-C335007
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts from HepG2 cells.
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CHEK1 / CHK1 Antibody (phospho-Ser280) LS-C335726
LifeSpan Biosciences
catalog: LS-C335726
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of MCF7 cells.
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CHEK1 / CHK1 Antibody (phospho-Ser317) LS-C335728
LifeSpan Biosciences
catalog: LS-C335728
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of extracts of MCF7 cells.

Immunofluorescence analysis of U2OS cells.
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IHC-plus™ CHEK1 / CHK1 Antibody (clone 2G1D5) LS-B11816
LifeSpan Biosciences
catalog: LS-B11816
mouse monoclonal (2G1D5)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

The anti-CHK1 antibody is used in Western blot to detect CHK1 in NIH/3T3 cell lysate (Lane 1) and K562 cell lysate (Lane 2).
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CHEK1 / CHK1 Antibody (phospho-Ser317) LS-C352005
LifeSpan Biosciences
catalog: LS-C352005
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of CHK1 (pS317) expression in HeLa (A); mouse kidney (B); rat heart (C) whole cell lysates.

Immunohistochemical analysis of CHK1 (pS317) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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CHEK1 / CHK1 Antibody (Internal) LS-C352006
LifeSpan Biosciences
catalog: LS-C352006
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of CHK1 expression in HeLa (A); mouse kidney (B); rat heart (C) whole cell lysates.

Immunohistochemical analysis of CHK1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of CHK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CHEK1 / CHK1 Antibody (phospho-Ser286) LS-C352007
LifeSpan Biosciences
catalog: LS-C352007
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of CHK1 (pS286) expression in HeLa (A); K562 (B); NIH3T3 (C); H9C2 (D) whole cell lysates.
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CHEK1 / CHK1 Antibody (Internal) LS-C352008
LifeSpan Biosciences
catalog: LS-C352008
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of CHK1 expression in HEK293T (A); NIH3T3 (B); rat heart (C) whole cell lysates.
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CHEK1 / CHK1 Antibody (Internal) LS-C352010
LifeSpan Biosciences
catalog: LS-C352010
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of CHK1 expression in HeLa (A); mouse kidney (B); rat heart (C) whole cell lysates.

Immunohistochemical analysis of CHK1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of CHK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
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CHEK1 / CHK1 Antibody (Internal) LS-C352014
LifeSpan Biosciences
catalog: LS-C352014
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of CHK1 expression in HeLa (A); mouse kidney (B); rat heart (C) whole cell lysates.

Immunohistochemical analysis of CHK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of CHK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CHEK1 / CHK1 Antibody (phospho-Ser296) LS-C357165
LifeSpan Biosciences
catalog: LS-C357165
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of extracts from HUVEC cells, treated with UV (15mins), using Chk1 (Phospho-Ser296) antibody.

Immunohistochemistry of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody.
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CHEK1 / CHK1 Antibody (phospho-Ser296) LS-C358940
LifeSpan Biosciences
catalog: LS-C358940
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of CHK1 (pS296) expression in HeLa UV-treated (A) whole cell lysates.

Immunohistochemical analysis of CHK1 (pS296) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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CHEK1 / CHK1 Antibody (phospho-Ser345) LS-C358948
LifeSpan Biosciences
catalog: LS-C358948
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of CHK1 (pS345) expression in HeLa (A); Jurkat (B); HL60 (C); mouse brain (D) whole cell lysates.

Immunohistochemical analysis of CHK1 (pS345) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of CHK1 (pS345) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CHEK1 / CHK1 Antibody (clone 2G1D5) LS-C359242
LifeSpan Biosciences
catalog: LS-C359242
mouse monoclonal (2G1D5)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
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CHEK1 / CHK1 Antibody (270-350 aa) LS-C380664
LifeSpan Biosciences
catalog: LS-C380664
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Chk1 (S317) antibody
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CHEK1 / CHK1 Antibody (220-300 aa) LS-C380668
LifeSpan Biosciences
catalog: LS-C380668
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot of Phospho-Chk1 (S280) antibody
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CHEK1 / CHK1 Antibody (280-360 aa) LS-C380670
LifeSpan Biosciences
catalog: LS-C380670
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry
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price:
to the supplier
CHEK1 / CHK1 Antibody (230-310 aa) LS-C381151
LifeSpan Biosciences
catalog: LS-C381151
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunocytochemistry
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price:
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CHEK1 / CHK1 Antibody (240-320 aa) LS-C381290
LifeSpan Biosciences
catalog: LS-C381290
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
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price:
to the supplier
CHEK1 / CHK1 Antibody (240-320 aa) LS-C382703
LifeSpan Biosciences
catalog: LS-C382703
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Chk1 antibody
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price:
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CHEK1 / CHK1 Antibody (270-350 aa) LS-C382704
LifeSpan Biosciences
catalog: LS-C382704
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Chk1 antibody
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price:
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CHEK1 / CHK1 Antibody (240-320 aa) LS-C382709
LifeSpan Biosciences
catalog: LS-C382709
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of Chk1 antibody
quantity:
price:
to the supplier
CHEK1 / CHK1 Antibody (280-360 aa) LS-C382710
LifeSpan Biosciences
catalog: LS-C382710
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Chk1 antibody
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price:
to the supplier