PIM2 / Pim-2 Antibody (aa277-308) LS-C99724
LifeSpan Biosciences
catalog: LS-C99724
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.

PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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PIM2 / Pim-2 Antibody (aa173-222) LS-C117088
LifeSpan Biosciences
catalog: LS-C117088
domestic rabbit polyclonal
reactivity: human
application: western blot
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PIM2 / Pim-2 Antibody (clone OTI8G10) LS-C115101
LifeSpan Biosciences
catalog: LS-C115101
mouse monoclonal (OTI8G10)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti- mouse monoclonal antibody. (Dilution 1:50).

Anti-PIM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.
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PIM2 / Pim-2 Antibody (clone OTI5B5) LS-C115102
LifeSpan Biosciences
catalog: LS-C115102
mouse monoclonal (OTI5B5)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.

IHC of paraffin-embedded Kidney tissue using anti- mouse monoclonal antibody. (Dilution 1:50).

Anti-PIM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.
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PIM2 / Pim-2 Antibody (clone OTI9A5) LS-C115200
LifeSpan Biosciences
catalog: LS-C115200
mouse monoclonal (OTI9A5)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.

IHC of paraffin-embedded Human colon tissue using anti-PIM2 mouse monoclonal antibody. (Dilution 1:50).

Anti-PIM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.
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PIM2 / Pim-2 Antibody (aa53-278) LS-C186336
LifeSpan Biosciences
catalog: LS-C186336
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

PIM2 antibody detects PIM2 protein by Western blot analysis. A. 50 ug mouse testis lysate/extract. 12 % SDS-PAGE. PIM2 antibody dilution:1:500

IHC of paraffin-embedded Breast ca using PIM2 antibody at 1:250 dilution.
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PIM2 / Pim-2 Antibody (aa277-308, AP) LS-C216773
LifeSpan Biosciences
catalog: LS-C216773
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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PIM2 / Pim-2 Antibody (aa277-308, APC) LS-C216774
LifeSpan Biosciences
catalog: LS-C216774
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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PIM2 / Pim-2 Antibody (aa277-308, Biotin) LS-C216775
LifeSpan Biosciences
catalog: LS-C216775
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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PIM2 / Pim-2 Antibody (aa277-308, FITC) LS-C216776
LifeSpan Biosciences
catalog: LS-C216776
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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PIM2 / Pim-2 Antibody (aa277-308, Azide-free, HRP) LS-C216777
LifeSpan Biosciences
catalog: LS-C216777
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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PIM2 / Pim-2 Antibody (aa277-308, PE) LS-C216778
LifeSpan Biosciences
catalog: LS-C216778
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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IHC-plus™ PIM2 / Pim-2 Antibody (aa287-299) LS-B10470
LifeSpan Biosciences
catalog: LS-B10470
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human, Testis: Formalin-Fixed Paraffin-Embedded (FFPE)

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with (0.1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (0.1mg/ml) in Lane B. Detec
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IHC-plus™ PIM2 / Pim-2 Antibody (aa23-36) LS-B10471
LifeSpan Biosciences
catalog: LS-B10471
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human, Heart: Formalin-Fixed Paraffin-Embedded (FFPE)

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with (0.1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (0.1mg/ml) in Lane B. Prima
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PIM2 / Pim-2 Antibody (aa98-319) LS-C295925
LifeSpan Biosciences
catalog: LS-C295925
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant PIM2 / Pim-2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PIM2 / Pim-2 Antibody (aa34-292) LS-C295926
LifeSpan Biosciences
catalog: LS-C295926
domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of recombinant PIM2 / Pim-2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PIM2 / Pim-2 Antibody (aa277-308) LS-C324052
LifeSpan Biosciences
catalog: LS-C324052
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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PIM2 / Pim-2 Antibody (aa82-291) LS-C373827
LifeSpan Biosciences
catalog: LS-C373827
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of PIM2 / Pim-2 antibody.

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PIM2 / Pim-2 Antibody (C-terminal) LS-C385513
LifeSpan Biosciences
catalog: LS-C385513
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
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