IHC-plus™ YWHAQ / 14-3-3 Theta Antibody (aa1-245, clone AT1A1) LS-B2967
LifeSpan Biosciences
catalog: LS-B2967
mouse monoclonal (AT1A1)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section


Anti-YWHAQ antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody LS-C26571
LifeSpan Biosciences
catalog: LS-C26571
mouse monoclonal
reactivity: human, bovine
application: western blot, ELISA
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa136-146) LS-C54425
LifeSpan Biosciences
catalog: LS-C54425
domestic goat polyclonal
reactivity: human
application: western blot

Antibody (0.03 ug/ml) staining of Human Brain (Hippocampus) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa196-245) LS-C117785
LifeSpan Biosciences
catalog: LS-C117785
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, using 14-3-3 thet/tau Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa41-90) LS-C119363
LifeSpan Biosciences
catalog: LS-C119363
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HUVEC and HeLa cells, using 14-3-3 thet Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa1-10) LS-C121322
LifeSpan Biosciences
catalog: LS-C121322
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (Trp228) LS-C176632
LifeSpan Biosciences
catalog: LS-C176632
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of 14-3-3 / (W228) pAb in extracts from HeLa cells.
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (Gln67) LS-C176847
LifeSpan Biosciences
catalog: LS-C176847
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of 14-3-3 (Q67) pAb in extracts from HUVEC cells.

IHC of 14-3-3 (Q67) pAb in paraffin-embedded human brain tissue.
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa100-150) LS-C289844
LifeSpan Biosciences
catalog: LS-C289844
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunoprecipitation
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa195-245) LS-C289845
LifeSpan Biosciences
catalog: LS-C289845
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (phospho-Ser232) LS-C291155
LifeSpan Biosciences
catalog: LS-C291155
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human pancreas, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The lane on the right is blocked with the phospho peptide.
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody LS-C335254
LifeSpan Biosciences
catalog: LS-C335254
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded mouse heart.

Immunofluorescence analysis of HeLa cells.
quantity:
price:
to the supplier
IHC-plus™ YWHAQ / 14-3-3 Theta Antibody (aa45-94) LS-B11976
LifeSpan Biosciences
catalog: LS-B11976
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (Preservative Free, Biotin) LS-C343170
LifeSpan Biosciences
catalog: LS-C343170
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (Preservative Free) LS-C343171
LifeSpan Biosciences
catalog: LS-C343171
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (C-Terminus) LS-C354150
LifeSpan Biosciences
catalog: LS-C354150
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of 14-3-3 theta/tau expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.

Immunohistochemical analysis of 14-3-3 theta/tau staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of 14-3-3 theta/tau staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (Internal) LS-C354151
LifeSpan Biosciences
catalog: LS-C354151
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of 14-3-3 theta expression in HeLa (A); MCF7 (B); SP2/0 (C); rat liver (D) whole cell lysates.

Immunohistochemical analysis of 14-3-3 theta staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of 14-3-3 theta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa125-175) LS-C359423
LifeSpan Biosciences
catalog: LS-C359423
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa40-90) LS-C359466
LifeSpan Biosciences
catalog: LS-C359466
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (C-Terminus) LS-C368640
LifeSpan Biosciences
catalog: LS-C368640
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of 14-3-3 theta/tau (pS232) expression in A549 (A); A431 (B); HEK293T (C); mouse brain (D) whole cell lysates.

Immunohistochemical analysis of 14-3-3 theta/tau (pS232) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of 14-3-3 theta/tau (pS232) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa1-241) LS-C374945
LifeSpan Biosciences
catalog: LS-C374945
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of YWHAQ / 14-3-3 Theta antibody.

quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa1-245) LS-C377016
LifeSpan Biosciences
catalog: LS-C377016
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: 14-3-3 protein theta antibody at 4 ug/ml. Lane 1: A549 whole cell lysate. Lane 2: mouse lung tissue. Lane 3: HepG2 whole cell lysate. secondary Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 28 kDa. Observed band size: 28 kDa.

Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (aa1-245) LS-C377018
LifeSpan Biosciences
catalog: LS-C377018
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (170-250 aa) LS-C381490
LifeSpan Biosciences
catalog: LS-C381490
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Phospho-14-3-3 theta/beta (S232) antibody
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (170-250 aa) LS-C381800
LifeSpan Biosciences
catalog: LS-C381800
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of 14-3-3 theta/beta antibody
quantity:
price:
to the supplier
YWHAQ / 14-3-3 Theta Antibody (10-90 aa, Internal) LS-C381803
LifeSpan Biosciences
catalog: LS-C381803
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of 14-3-3 theta antibody
quantity:
price:
to the supplier