DBF4 Antibody LS-C64525
LifeSpan Biosciences
catalog: LS-C64525

mouse monoclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

DBF4 Antibody (aa10-59) LS-C119300
LifeSpan Biosciences
catalog: LS-C119300

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from NIH/3T3 cells, treated with H2O2 100uM 30', COS7 treated with PMA 125ng/ml 30',using DBF4 Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier

DBF4 Antibody (clone 6G9) LS-C133665
LifeSpan Biosciences
catalog: LS-C133665

mouse monoclonal (6G9)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.

Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].

Detection limit for recombinant GST tagged DBF4 is approximately 0.1 ng/ml as a capture antibody.
quantity:
price:
to the supplier

DBF4 Antibody (aa585-599) LS-C139824
LifeSpan Biosciences
catalog: LS-C139824

domestic goat polyclonal
reactivity: human, mouse
application: western blot

DBF4 antibody (1 ug/ml) staining of Mouse Testis lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
quantity:
price:
to the supplier

DBF4 Antibody (aa581-630) LS-C151281
LifeSpan Biosciences
catalog: LS-C151281

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

quantity:
price:
to the supplier

DBF4 Antibody (Ile18) LS-C176953
LifeSpan Biosciences
catalog: LS-C176953

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of Dbf4 (I18) pAb in extracts from NIH-3T3 treated with H2O2 100uM 30'.
quantity:
price:
to the supplier

DBF4 Antibody (clone 2047C4a) LS-C184322
LifeSpan Biosciences
catalog: LS-C184322

mouse monoclonal (2047C4a)
reactivity: human

quantity:
price:
to the supplier

DBF4 Antibody LS-C310083
LifeSpan Biosciences
catalog: LS-C310083

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

DBF4 Antibody (N-Terminus) LS-C353288
LifeSpan Biosciences
catalog: LS-C353288

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of DBF4 expression in Jurkat (A); HepG2 (B); SW480 (C) whole cell lysates.

Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of DBF4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier

DBF4 Antibody (10-90 aa, N-terminal) LS-C383084
LifeSpan Biosciences
catalog: LS-C383084

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Dbf4 antibody
quantity:
price:
to the supplier