IHC-plus™ Apg7 / ATG7 Antibody LS-B2195
LifeSpan Biosciences
catalog: LS-B2195
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.

Immunofluorescence of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.
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Apg7 / ATG7 Antibody (aa284-313) LS-C156616
LifeSpan Biosciences
catalog: LS-C156616
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-ATG7 Antibody antibody in 293 cell line lysates transiently transfected with the ATG7 gene (2 ug/lane). hAPG7L-P299(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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IHC-plus™ Apg7 / ATG7 Antibody LS-B330
LifeSpan Biosciences
catalog: LS-B330
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-ATG7 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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IHC-plus™ Apg7 / ATG7 Antibody LS-B2194
LifeSpan Biosciences
catalog: LS-B2194
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-ATG7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of APG7 in MCF7 cells with APG7 antibody at 10 ug/ml.

Immunofluorescence of APG7 in MCF7 cells with APG7 antibody at 20 ug/ml.
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IHC-plus™ Apg7 / ATG7 Antibody (C-Terminus) LS-B2216
LifeSpan Biosciences
catalog: LS-B2216
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ Apg7 / ATG7 Antibody (N-Terminus) LS-B2217
LifeSpan Biosciences
catalog: LS-B2217
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Apg7 / ATG7 Antibody (N-Terminus) LS-C69111
LifeSpan Biosciences
catalog: LS-C69111
domestic rabbit polyclonal
reactivity: human
application: western blot
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Apg7 / ATG7 Antibody LS-C93848
LifeSpan Biosciences
catalog: LS-C93848
domestic sheep polyclonal
reactivity: human
application: western blot, immunohistochemistry
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Apg7 / ATG7 Antibody LS-C147030
LifeSpan Biosciences
catalog: LS-C147030
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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Apg7 / ATG7 Antibody (aa550-600) LS-C148584
LifeSpan Biosciences
catalog: LS-C148584
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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Apg7 / ATG7 Antibody (aa540-569) LS-C156407
LifeSpan Biosciences
catalog: LS-C156407
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

hAPG7L-D555 (PEI 1:100)b western blot of HeLa cell line lysates (35 ug/lane). The APG7L antibody detected the APG7L protein (arrow).

APG7L Antibody(D555) immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

APG7L Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Apg7 / ATG7 Antibody LS-C156612
LifeSpan Biosciences
catalog: LS-C156612
mouse monoclonal
reactivity: human
application: western blot

Western blot of anti-APG7 Monoclonal Antibody by Recombinant APG7 protein (Fragment 34KD). APG7 protein (Fragment 34KD)(arrow) was detected using the ascites antibody.
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Apg7 / ATG7 Antibody (aa494-523) LS-C156613
LifeSpan Biosciences
catalog: LS-C156613
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Fluorescent image of U251 cells stained with ATG7 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG7 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000197548).

Western blot of anti-hAPG7L-R509 antibody in 293 cell line lysates transiently transfected with the ATG7 gene (2 ug/lane). hAPG7L-R509(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Apg7 / ATG7 Antibody (aa540-569) LS-C156615
LifeSpan Biosciences
catalog: LS-C156615
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence of U251 cells, using ATG7 Antibody. U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Western blot of APG7L antibody in 293 cell line lysates transiently transfected with the ATG7 gene(2 ug/lane).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG7L antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Apg7 / ATG7 Antibody (aa22-51) LS-C156617
LifeSpan Biosciences
catalog: LS-C156617
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-Autophagy APG7L Antibody in 293 cell line lysates transiently transfected with the ATG7 gene (3 ug/lane). APG7L (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Apg7 / ATG7 Antibody (aa489-538) LS-C170299
LifeSpan Biosciences
catalog: LS-C170299
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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Apg7 / ATG7 Antibody (aa540-569, APC) LS-C251508
LifeSpan Biosciences
catalog: LS-C251508
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Apg7 / ATG7 Antibody (aa540-569, AP) LS-C251509
LifeSpan Biosciences
catalog: LS-C251509
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Apg7 / ATG7 Antibody (aa540-569, Biotin) LS-C251510
LifeSpan Biosciences
catalog: LS-C251510
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Apg7 / ATG7 Antibody (aa540-569, FITC) LS-C251511
LifeSpan Biosciences
catalog: LS-C251511
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Apg7 / ATG7 Antibody (aa540-569, Azide-free, HRP) LS-C251512
LifeSpan Biosciences
catalog: LS-C251512
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Apg7 / ATG7 Antibody (aa540-569, PE) LS-C251514
LifeSpan Biosciences
catalog: LS-C251514
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Apg7 / ATG7 Antibody (Ser95, AP) LS-C251938
LifeSpan Biosciences
catalog: LS-C251938
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry
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Apg7 / ATG7 Antibody (Ser95, APC) LS-C251939
LifeSpan Biosciences
catalog: LS-C251939
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry
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Apg7 / ATG7 Antibody (Ser95, Biotin) LS-C251940
LifeSpan Biosciences
catalog: LS-C251940
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry
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Apg7 / ATG7 Antibody (Ser95, FITC) LS-C251941
LifeSpan Biosciences
catalog: LS-C251941
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry
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Apg7 / ATG7 Antibody (Ser95, Azide-free, HRP) LS-C251942
LifeSpan Biosciences
catalog: LS-C251942
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry
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Apg7 / ATG7 Antibody (Ser95, PE) LS-C251947
LifeSpan Biosciences
catalog: LS-C251947
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry
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Apg7 / ATG7 Antibody (clone 29CT10.3.12, AP) LS-C259996
LifeSpan Biosciences
catalog: LS-C259996
mouse monoclonal (29CT10.3.12)
reactivity: human
conjugate: AP
application: western blot, ELISA
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Apg7 / ATG7 Antibody (clone 29CT10.3.12, APC) LS-C259997
LifeSpan Biosciences
catalog: LS-C259997
mouse monoclonal (29CT10.3.12)
reactivity: human
conjugate: APC
application: western blot, ELISA
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Apg7 / ATG7 Antibody (clone 29CT10.3.12, Biotin) LS-C259998
LifeSpan Biosciences
catalog: LS-C259998
mouse monoclonal (29CT10.3.12)
reactivity: human
conjugate: biotin
application: western blot, ELISA
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Apg7 / ATG7 Antibody (clone 29CT10.3.12, FITC) LS-C259999
LifeSpan Biosciences
catalog: LS-C259999
mouse monoclonal (29CT10.3.12)
reactivity: human
conjugate: FITC
application: western blot, ELISA
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Apg7 / ATG7 Antibody (clone 29CT10.3.12, PE) LS-C260000
LifeSpan Biosciences
catalog: LS-C260000
mouse monoclonal (29CT10.3.12)
reactivity: human
conjugate: PE
application: western blot, ELISA
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Apg7 / ATG7 Antibody (clone 29CT10.3.12, Azide-free, HRP) LS-C260001
LifeSpan Biosciences
catalog: LS-C260001
mouse monoclonal (29CT10.3.12)
reactivity: human
conjugate: HRP
application: western blot, ELISA
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Apg7 / ATG7 Antibody LS-C306433
LifeSpan Biosciences
catalog: LS-C306433
mouse polyclonal
reactivity: human
application: western blot
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Apg7 / ATG7 Antibody LS-C309547
LifeSpan Biosciences
catalog: LS-C309547
domestic rabbit polyclonal
reactivity: human
application: western blot
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Apg7 / ATG7 Antibody (aa347-581) LS-C311792
LifeSpan Biosciences
catalog: LS-C311792
domestic rabbit polyclonal
reactivity: human
application: western blot
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Apg7 / ATG7 Antibody (aa652-669) LS-C313355
LifeSpan Biosciences
catalog: LS-C313355
domestic rabbit polyclonal
reactivity: human
application: western blot

WB of Apg7 / ATG7 antibody. All lanes: Anti-ATG7 at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 40ug. Lane 2: Rat Kidney Tissue Lysate at 40ug. Lane 3: Human Placenta Tissue Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Lane 6: 293T Whole Cell Lysate at 40ug. Lane 7: HEPG2 Whole Cell Lysate at 40ug. Predicted bind size: 78KD. Observed bind size: 78KD.
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Apg7 / ATG7 Antibody LS-C192267
LifeSpan Biosciences
catalog: LS-C192267
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of ATG7 pAb in extracts from 293T cells.
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Apg7 / ATG7 Antibody (aa540-569) LS-C319529
LifeSpan Biosciences
catalog: LS-C319529
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
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Apg7 / ATG7 Antibody (Ser95) LS-C319672
LifeSpan Biosciences
catalog: LS-C319672
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
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Apg7 / ATG7 Antibody (clone 29CT10.3.12) LS-C329152
LifeSpan Biosciences
catalog: LS-C329152
mouse monoclonal (29CT10.3.12)
reactivity: human
application: western blot, ELISA
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Apg7 / ATG7 Antibody LS-C331071
LifeSpan Biosciences
catalog: LS-C331071
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using ATG7 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.

Immunohistochemistry of paraffin-embedded human tonsil using ATG7 antibodyat dilution of 1:100 (40x lens).
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Apg7 / ATG7 Antibody (aa477-698) LS-C373757
LifeSpan Biosciences
catalog: LS-C373757
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of Apg7 / ATG7 antibody.

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