IHC-plus™ YAP / YAP1 Antibody (clone 2F12) LS-B4494
LifeSpan Biosciences
catalog: LS-B4494

mouse monoclonal (2F12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of YAP1 expression in transfected 293T cell line by YAP1 monoclonal antibody clone 2F12.

Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell (antibody concentration 30 ug/ml).

Detection limit for recombinant GST tagged YAP1 is approximately 0.03 ng/ml as a capture antibody.
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IHC-plus™ YAP / YAP1 Antibody (aa351-400) LS-B3561
LifeSpan Biosciences
catalog: LS-B3561

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-YAP1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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YAP / YAP1 Antibody LS-C48734
LifeSpan Biosciences
catalog: LS-C48734

domestic rabbit polyclonal
reactivity: human
application: western blot

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YAP / YAP1 Antibody LS-C90803
LifeSpan Biosciences
catalog: LS-C90803

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

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YAP / YAP1 Antibody (aa420-446) LS-C99293
LifeSpan Biosciences
catalog: LS-C99293

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot of YAP1 antibody in NCI-H460 cell line lysates (35 ug/lane). YAP1 (arrow) was detected using the purified antibody.

YAP1 antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC-plus™ YAP / YAP1 Antibody (clone 2H1) LS-B4495
LifeSpan Biosciences
catalog: LS-B4495

mouse monoclonal (2H1)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

YAP1 monoclonal antibody, clone 2H1 Western blot of YAP1 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell (antibody concentration 40 ug/ml).

Detection limit for recombinant GST tagged YAP1 is approximately 0.03 ng/ml as a capture antibody.
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YAP / YAP1 Antibody (aa281-330) LS-C118901
LifeSpan Biosciences
catalog: LS-C118901

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HeLa, HepG2, and COLO205 cells, using YAP Antibody. The lane on the right is blocked with the synthesized peptide.
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YAP / YAP1 Antibody LS-C153509
LifeSpan Biosciences
catalog: LS-C153509

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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YAP / YAP1 Antibody (aa104-133) LS-C160678
LifeSpan Biosciences
catalog: LS-C160678

domestic rabbit polyclonal
reactivity: human
application: western blot

YAP Antibody (S127) western blot of MDA-MB231 cell line lysates (35 ug/lane). The YAP antibody detected the YAP protein (arrow).
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YAP / YAP1 Antibody (phospho-Ser127) LS-C163682
LifeSpan Biosciences
catalog: LS-C163682

domestic rabbit polyclonal
reactivity: human

Dot blot of Phospho-YAP-S127 antibody Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
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YAP / YAP1 Antibody (His122) LS-C176565
LifeSpan Biosciences
catalog: LS-C176565

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of YAP (H122) pAb in extracts from HepG2 cells.

IHC of YAP (H122) pAb in paraffin-embedded human heart tissue.
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YAP / YAP1 Antibody (Gln295) LS-C176767
LifeSpan Biosciences
catalog: LS-C176767

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of YAP (Q295) pAb in extracts from HeLa cells.

IHC of YAP (Q295) pAb in paraffin-embedded human breast carcinoma tissue.
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IHC-plus™ YAP / YAP1 Antibody (clone 1A12) LS-B8943
LifeSpan Biosciences
catalog: LS-B8943

mouse monoclonal (1A12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-YAP / YAP1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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IHC-plus™ YAP / YAP1 Antibody (aa93-142) LS-B9238
LifeSpan Biosciences
catalog: LS-B9238

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-YAP / YAP1 antibody IHC staining of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot analysis of lysates from HepG2 cells, treated with Wortmannin 40nM 24h, using YAP Antibody. The lane on the right is blocked with the synthesized peptide.
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YAP / YAP1 Antibody (phospho-Ser127) LS-C199738
LifeSpan Biosciences
catalog: LS-C199738

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right is blocked with the phospho peptide.
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YAP / YAP1 Antibody (phospho-Ser127, APC) LS-C273771
LifeSpan Biosciences
catalog: LS-C273771

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA

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YAP / YAP1 Antibody (phospho-Ser127, AP) LS-C273772
LifeSpan Biosciences
catalog: LS-C273772

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA

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YAP / YAP1 Antibody (phospho-Ser127, PE) LS-C273773
LifeSpan Biosciences
catalog: LS-C273773

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA

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YAP / YAP1 Antibody (aa104-133, AP) LS-C273774
LifeSpan Biosciences
catalog: LS-C273774

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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YAP / YAP1 Antibody (aa104-133, APC) LS-C273775
LifeSpan Biosciences
catalog: LS-C273775

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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YAP / YAP1 Antibody (aa104-133, FITC) LS-C273776
LifeSpan Biosciences
catalog: LS-C273776

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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YAP / YAP1 Antibody (aa104-133, PE) LS-C273777
LifeSpan Biosciences
catalog: LS-C273777

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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YAP / YAP1 Antibody (phospho-Ser127, FITC) LS-C273831
LifeSpan Biosciences
catalog: LS-C273831

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA

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YAP / YAP1 Antibody (aa250-300) LS-C289061
LifeSpan Biosciences
catalog: LS-C289061

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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YAP / YAP1 Antibody (aa104-133) LS-C326407
LifeSpan Biosciences
catalog: LS-C326407

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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YAP / YAP1 Antibody (phospho-Ser127) LS-C326408
LifeSpan Biosciences
catalog: LS-C326408

domestic rabbit polyclonal
reactivity: human
application: ELISA

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YAP / YAP1 Antibody LS-C331201
LifeSpan Biosciences
catalog: LS-C331201

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of A549 cells.

Immunoprecipitation analysis of 150ug extracts of HeLa cells.
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YAP / YAP1 Antibody (Internal) LS-C353263
LifeSpan Biosciences
catalog: LS-C353263

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of YAP1 expression in SW480 (A); HeLa (B); Caco2 (C) whole cell lysates.

Immunohistochemical analysis of YAP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of YAP1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IHC-plus™ YAP / YAP1 Antibody (aa350-400) LS-B12796
LifeSpan Biosciences
catalog: LS-B12796

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

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YAP / YAP1 Antibody (phospho-Ser127) LS-C358850
LifeSpan Biosciences
catalog: LS-C358850

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of YAP1 (pS127) expression in MCF7 (A); PC3 (B) whole cell lysates.
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YAP / YAP1 Antibody (clone 1A12) LS-C359315
LifeSpan Biosciences
catalog: LS-C359315

mouse monoclonal (1A12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

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YAP / YAP1 Antibody (70-150 aa) LS-C381301
LifeSpan Biosciences
catalog: LS-C381301

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-YAP (S127) antibody
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YAP / YAP1 Antibody (70-150 aa) LS-C386693
LifeSpan Biosciences
catalog: LS-C386693

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of YAP antibody
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YAP / YAP1 Antibody (250-330 aa, Internal) LS-C386696
LifeSpan Biosciences
catalog: LS-C386696

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of YAP antibody
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