AKR1A1 Antibody (clone 1A11-2A4) LS-C133637
LifeSpan Biosciences
catalog: LS-C133637
mouse monoclonal (1A11-2A4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of AKR1A1 expression in transfected 293T cell line by AKR1A1 monoclonal antibody (M01), clone 1A11-2A4.

Immunoperoxidase of monoclonal antibody to AKR1A1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.75 ug/ml]

Detection limit for recombinant GST tagged AKR1A1 is approximately 0.1 ng/ml as a capture antibody.
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AKR1A1 Antibody LS-C137236
LifeSpan Biosciences
catalog: LS-C137236
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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AKR1A1 Antibody (aa293-325) LS-C98605
LifeSpan Biosciences
catalog: LS-C98605
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot of AKR1A1 (arrow) using rabbit polyclonal AKR1A1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1A1 gene.
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AKR1A1 Antibody LS-C136832
LifeSpan Biosciences
catalog: LS-C136832
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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AKR1A1 Antibody (C-Terminus) LS-C151742
LifeSpan Biosciences
catalog: LS-C151742
domestic rabbit polyclonal
reactivity: human
application: western blot
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AKR1A1 Antibody (aa50-99) LS-C151743
LifeSpan Biosciences
catalog: LS-C151743
domestic rabbit polyclonal
reactivity: human
application: western blot
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AKR1A1 Antibody (clone OTI9F1) LS-C114818
LifeSpan Biosciences
catalog: LS-C114818
mouse monoclonal (OTI9F1)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.

IHC of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody. (Dilution 1:50).

Flow cytometry of Jurkat cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
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AKR1A1 Antibody (clone OTI8E12) LS-C114819
LifeSpan Biosciences
catalog: LS-C114819
mouse monoclonal (OTI8E12)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.

IHC of paraffin-embedded Kidney tissue using anti-AKR1A1 mouse monoclonal antibody. (Dilution 1:50).

Flow cytometry of HeLa cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
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AKR1A1 Antibody (clone OTI2E7) LS-C114820
LifeSpan Biosciences
catalog: LS-C114820
mouse monoclonal (OTI2E7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.

IHC of paraffin-embedded colon tissue using anti-AKR1A1 mouse monoclonal antibody. (Dilution 1:50).
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AKR1A1 Antibody (clone OTI8H2) LS-C114821
LifeSpan Biosciences
catalog: LS-C114821
mouse monoclonal (OTI8H2)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.

IHC of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody. (Dilution 1:50).

Flow cytometry of HeLa cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
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AKR1A1 Antibody (clone OTI6E3) LS-C114823
LifeSpan Biosciences
catalog: LS-C114823
mouse monoclonal (OTI6E3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.

IHC of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody. (Dilution 1:50).

Anti-AKR1A1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.
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AKR1A1 Antibody (clone OTI4G2) LS-C114824
LifeSpan Biosciences
catalog: LS-C114824
mouse monoclonal (OTI4G2)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.

IHC of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody. (Dilution 1:50).

Flow cytometry of HeLa cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
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AKR1A1 Antibody (clone OTI3C7) LS-C114845
LifeSpan Biosciences
catalog: LS-C114845
mouse monoclonal (OTI3C7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.

IHC of paraffin-embedded Kidney tissue using anti-AKR1A1 mouse monoclonal antibody. (Dilution 1:50).
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AKR1A1 Antibody (clone OTI4G6) LS-C114885
LifeSpan Biosciences
catalog: LS-C114885
mouse monoclonal (OTI4G6)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.

IHC of paraffin-embedded Kidney tissue using anti-AKR1A1 mouse monoclonal antibody. (Dilution 1:50).
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AKR1A1 Antibody LS-C306203
LifeSpan Biosciences
catalog: LS-C306203
mouse polyclonal
reactivity: human
application: western blot
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AKR1A1 Antibody LS-C309415
LifeSpan Biosciences
catalog: LS-C309415
domestic rabbit polyclonal
reactivity: human
application: western blot
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AKR1A1 Antibody LS-C313662
LifeSpan Biosciences
catalog: LS-C313662
domestic rabbit polyclonal
reactivity: human
application: western blot
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AKR1A1 Antibody LS-C192341
LifeSpan Biosciences
catalog: LS-C192341
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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AKR1A1 Antibody LS-C331236
LifeSpan Biosciences
catalog: LS-C331236
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemistry of paraffin-embedded rat kidney tissue.

Immunofluorescence analysis of HeLa cells.

Western blot analysis of extracts of various cell lines, using AKR1A1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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AKR1A1 Antibody (N-Terminus) LS-C345758
LifeSpan Biosciences
catalog: LS-C345758
domestic rabbit polyclonal
reactivity: human
application: western blot
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AKR1A1 Antibody (C-Terminus) LS-C347627
LifeSpan Biosciences
catalog: LS-C347627
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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AKR1A1 Antibody (C-Terminus) LS-C353248
LifeSpan Biosciences
catalog: LS-C353248
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of AKR1A1 expression in HeLa (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
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AKR1A1 Antibody LS-C357048
LifeSpan Biosciences
catalog: LS-C357048
domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-AKR1A1 Antibody at 1:1000 dilution + human kidney lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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AKR1A1 Antibody (250-330 aa, C-terminal) LS-C381964
LifeSpan Biosciences
catalog: LS-C381964
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of AKR1A1 antibody
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