IHC-plus™ PAK4 Antibody LS-B538
LifeSpan Biosciences
catalog: LS-B538
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-PAK4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of PAK4 in Human Colon cells with PAK4 antibody at 20 ug/ml.

Western blot of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 ug/ml.
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PAK4 Antibody (N-Terminus) LS-C48425
LifeSpan Biosciences
catalog: LS-C48425
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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PAK4 Antibody (N-Terminus) LS-C9450
LifeSpan Biosciences
catalog: LS-C9450
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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PAK4 Antibody (aa1-120) LS-C38050
LifeSpan Biosciences
catalog: LS-C38050
domestic goat polyclonal
reactivity: human
application: western blot
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PAK4 Antibody (aa156-187) LS-C99719
LifeSpan Biosciences
catalog: LS-C99719
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PAK4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

The anti-PAK4 antibody is used in Western blot to detect PAK4 in mouse small intestine tissue lysate.
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PAK4 Antibody (aa441-490) LS-C117768
LifeSpan Biosciences
catalog: LS-C117768
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from K562 cells, treated with PMA 125ng/ml 30', using PAK4/5/6 Antibody. The lane on the right is blocked with the synthesized peptide.
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PAK4 Antibody (aa23-72) LS-C135211
LifeSpan Biosciences
catalog: LS-C135211
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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PAK4 Antibody (aa268-317) LS-C201548
LifeSpan Biosciences
catalog: LS-C201548
domestic rabbit polyclonal
reactivity: human
application: western blot
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PAK4 Antibody (aa187-216, AP) LS-C223584
LifeSpan Biosciences
catalog: LS-C223584
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, flow cytometry
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PAK4 Antibody (aa187-216, APC) LS-C223585
LifeSpan Biosciences
catalog: LS-C223585
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, flow cytometry
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PAK4 Antibody (aa187-216, Biotin) LS-C223586
LifeSpan Biosciences
catalog: LS-C223586
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, flow cytometry
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PAK4 Antibody (aa187-216, FITC) LS-C223587
LifeSpan Biosciences
catalog: LS-C223587
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, flow cytometry
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PAK4 Antibody (aa187-216, Azide-free, HRP) LS-C223588
LifeSpan Biosciences
catalog: LS-C223588
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, flow cytometry
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PAK4 Antibody (aa187-216, PE) LS-C223590
LifeSpan Biosciences
catalog: LS-C223590
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, flow cytometry
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PAK4 Antibody (aa156-187, AP) LS-C226089
LifeSpan Biosciences
catalog: LS-C226089
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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PAK4 Antibody (aa156-187, APC) LS-C226090
LifeSpan Biosciences
catalog: LS-C226090
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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PAK4 Antibody (aa156-187, Biotin) LS-C226091
LifeSpan Biosciences
catalog: LS-C226091
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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PAK4 Antibody (aa156-187, FITC) LS-C226092
LifeSpan Biosciences
catalog: LS-C226092
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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PAK4 Antibody (aa156-187, Azide-free, HRP) LS-C226093
LifeSpan Biosciences
catalog: LS-C226093
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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PAK4 Antibody (aa156-187, PE) LS-C226097
LifeSpan Biosciences
catalog: LS-C226097
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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IHC-plus™ PAK4 Antibody (aa187-216) LS-B10177
LifeSpan Biosciences
catalog: LS-B10177
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-PAK4 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Mouse Pak4 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The Pak4 antibody detected the Pak4 protein (arrow).

Mouse Pak4 Antibody flow cytometry of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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PAK4 Antibody (aa25-75) LS-C287254
LifeSpan Biosciences
catalog: LS-C287254
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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PAK4 Antibody (aa299-542) LS-C295748
LifeSpan Biosciences
catalog: LS-C295748
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Knockout Varification: Lane 1: Wild-type Hela cell lysate; Lane 2: PAK4 knockout Hela cell lysate; Predicted MW: 64kDa Observed MW: 70kDa Primary Ab: 1µg/ml Rabbit Anti-Human PAK4 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PAK4 + PAK5 + PAK6 Antibody (phospho-Ser474) LS-C313619
LifeSpan Biosciences
catalog: LS-C313619
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry
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PAK4 Antibody (aa187-216) LS-C323764
LifeSpan Biosciences
catalog: LS-C323764
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, flow cytometry
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PAK4 Antibody (aa156-187) LS-C328138
LifeSpan Biosciences
catalog: LS-C328138
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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PAK4 Antibody LS-C332245
LifeSpan Biosciences
catalog: LS-C332245
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using PAK4 antibody.

Immunohistochemistry of paraffin-embedded human tonsil tissue using PAK4 antibody.
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PAK4 Antibody (aa1-308, clone OTI1C7) LS-C340233
LifeSpan Biosciences
catalog: LS-C340233
mouse monoclonal (OTI1C7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAK4.

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PAK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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PAK4 Antibody (aa1-308, clone OTI1C11) LS-C340235
LifeSpan Biosciences
catalog: LS-C340235
mouse monoclonal (OTI1C11)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAK4.
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PAK4 Antibody (aa1-308, clone OTI3C3) LS-C340250
LifeSpan Biosciences
catalog: LS-C340250
mouse monoclonal (OTI3C3)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAK4.
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PAK4 Antibody (phospho-Ser474) LS-C353257
LifeSpan Biosciences
catalog: LS-C353257
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Immunofluorescent analysis of PAK4 (pS474) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of PAK4 (pS474) expression in HEK293T EGF-treated (A); Raw264.7 EGF-treated (B); PC12 EGF-treated (C) whole cell lysates. Ab used at a 1:500 dilution.
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PAK4 Antibody (FITC) LS-C370617
LifeSpan Biosciences
catalog: LS-C370617
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human prostate using antibody at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PAK4 Antibody LS-C370618
LifeSpan Biosciences
catalog: LS-C370618
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human prostate using antibody at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PAK4 Antibody (Biotin) LS-C370619
LifeSpan Biosciences
catalog: LS-C370619
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human prostate using antibody at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PAK4 Antibody (HRP) LS-C370620
LifeSpan Biosciences
catalog: LS-C370620
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human prostate using antibody at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PAK4 Antibody (410-490 aa) LS-C381564
LifeSpan Biosciences
catalog: LS-C381564
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
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