BCKDK Antibody (aa120-151) LS-C97773
LifeSpan Biosciences
catalog: LS-C97773

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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BCKDK Antibody (aa325-356) LS-C98050
LifeSpan Biosciences
catalog: LS-C98050

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody (C-term T340) (RB05257). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies).
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BCKDK Antibody (aa11-60) LS-C118664
LifeSpan Biosciences
catalog: LS-C118664

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using BCKD Antibody . The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat and 293 cells, using BCKD Antibody . The lane on the right is blocked with the synthesized peptide.
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BCKDK Antibody (N-Terminus) LS-C146419
LifeSpan Biosciences
catalog: LS-C146419

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

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BCKDK Antibody (aa159-188) LS-C164302
LifeSpan Biosciences
catalog: LS-C164302

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Bckdk Antibody western blot of HepG2 cell line lysates (35 ug/lane). The Bckdk antibody detected the Bckdk protein (arrow).
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BCKDK Antibody (clone OTI11C9) LS-C114777
LifeSpan Biosciences
catalog: LS-C114777

mouse monoclonal (OTI11C9)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK.

IHC of paraffin-embedded Human Ovary tissue using anti-BCKDK mouse monoclonal antibody.

Anti-BCKDK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCKDK.
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BCKDK Antibody (Glu41) LS-C191919
LifeSpan Biosciences
catalog: LS-C191919

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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BCKDK Antibody (aa159-188, AP) LS-C252439
LifeSpan Biosciences
catalog: LS-C252439

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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BCKDK Antibody (aa159-188, APC) LS-C252440
LifeSpan Biosciences
catalog: LS-C252440

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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BCKDK Antibody (aa159-188, Biotin) LS-C252441
LifeSpan Biosciences
catalog: LS-C252441

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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BCKDK Antibody (aa159-188, FITC) LS-C252442
LifeSpan Biosciences
catalog: LS-C252442

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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BCKDK Antibody (aa159-188, Azide-free, HRP) LS-C252443
LifeSpan Biosciences
catalog: LS-C252443

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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BCKDK Antibody (aa159-188, PE) LS-C252444
LifeSpan Biosciences
catalog: LS-C252444

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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BCKDK Antibody (aa325-356, AP) LS-C275437
LifeSpan Biosciences
catalog: LS-C275437

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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BCKDK Antibody (aa325-356, APC) LS-C275438
LifeSpan Biosciences
catalog: LS-C275438

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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BCKDK Antibody (aa325-356, Biotin) LS-C275439
LifeSpan Biosciences
catalog: LS-C275439

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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BCKDK Antibody (aa325-356, FITC) LS-C275440
LifeSpan Biosciences
catalog: LS-C275440

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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BCKDK Antibody (aa325-356, Azide-free, HRP) LS-C275441
LifeSpan Biosciences
catalog: LS-C275441

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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BCKDK Antibody (aa325-356, PE) LS-C275442
LifeSpan Biosciences
catalog: LS-C275442

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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BCKDK Antibody LS-C306525
LifeSpan Biosciences
catalog: LS-C306525

mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry

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BCKDK Antibody (aa159-188) LS-C319799
LifeSpan Biosciences
catalog: LS-C319799

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

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BCKDK Antibody (aa325-356) LS-C326914
LifeSpan Biosciences
catalog: LS-C326914

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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BCKDK Antibody (N-Terminus) LS-C353246
LifeSpan Biosciences
catalog: LS-C353246

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of BCKDK expression in Raji (A); HeLa (B); Jurkat (C); SHSY5Y (D) whole cell lysates.

Immunohistochemical analysis of BCKDK staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of BCKDK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IHC-plus™ BCKDK Antibody (aa111-160) LS-B12637
LifeSpan Biosciences
catalog: LS-B12637

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Colon, Submucosal Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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BCKDK Antibody (aa153-412) LS-C375311
LifeSpan Biosciences
catalog: LS-C375311

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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BCKDK Antibody (aa153-412) LS-C375313
LifeSpan Biosciences
catalog: LS-C375313

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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BCKDK Antibody (30-110 aa, N-terminal) LS-C382252
LifeSpan Biosciences
catalog: LS-C382252

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of BCKDK antibody
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