CDK4 Antibody (clone DCS-156) LS-C22048
LifeSpan Biosciences
catalog: LS-C22048
mouse monoclonal (DCS-156)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
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CDK4 Antibody (C-Terminus) LS-C18774
LifeSpan Biosciences
catalog: LS-C18774
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation
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CDK4 Antibody (aa279-300) LS-C63193
LifeSpan Biosciences
catalog: LS-C63193
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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CDK4 Antibody (aa1-20) LS-C22031
LifeSpan Biosciences
catalog: LS-C22031
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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CDK4 Antibody (C-Terminus) LS-C22047
LifeSpan Biosciences
catalog: LS-C22047
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation
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CDK4 Antibody (clone DCS-35) LS-C22049
LifeSpan Biosciences
catalog: LS-C22049
mouse monoclonal (DCS-35)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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CDK4 Antibody LS-C22051
LifeSpan Biosciences
catalog: LS-C22051
mouse monoclonal
reactivity: human
application: western blot, immunoprecipitation
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CDK4 Antibody (C-Terminus) LS-C30735
LifeSpan Biosciences
catalog: LS-C30735
domestic rabbit polyclonal
reactivity: human
application: western blot
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CDK4 Antibody LS-C26669
LifeSpan Biosciences
catalog: LS-C26669
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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CDK4 Antibody (clone DCS-35) LS-C88038
LifeSpan Biosciences
catalog: LS-C88038
mouse monoclonal (DCS-35)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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CDK4 Antibody (clone DCS-35, Biotin) LS-C88039
LifeSpan Biosciences
catalog: LS-C88039
mouse monoclonal (DCS-35)
reactivity: human
conjugate: biotin
application: western blot, immunocytochemistry, immunoprecipitation
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CDK4 Antibody (clone DCS-35, Azide-free) LS-C88040
LifeSpan Biosciences
catalog: LS-C88040
mouse monoclonal (DCS-35)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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CDK4 Antibody (aa291-303) LS-C88987
LifeSpan Biosciences
catalog: LS-C88987
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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CDK4 Antibody LS-C88988
LifeSpan Biosciences
catalog: LS-C88988
domestic sheep polyclonal
reactivity: human
application: ELISA
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CDK4 Antibody (aa273-305) LS-C99873
LifeSpan Biosciences
catalog: LS-C99873
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Fluorescent image of HeLa cell stained with CDK4 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDK4 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDK4 immunoreactivity is localized to Cytoplasm significantly.

Western blot of CDK4 (arrow) using rabbit polyclonal CDK4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK4 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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CDK4 Antibody LS-C128056
LifeSpan Biosciences
catalog: LS-C128056
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry
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CDK4 Antibody (aa1-303) LS-C128058
LifeSpan Biosciences
catalog: LS-C128058
domestic goat polyclonal
reactivity: human
application: western blot
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CDK4 Antibody LS-C137013
LifeSpan Biosciences
catalog: LS-C137013
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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CDK4 Antibody (C-Terminus) LS-C145969
LifeSpan Biosciences
catalog: LS-C145969
domestic rabbit polyclonal
reactivity: human
application: western blot
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CDK4 Antibody (aa219-246) LS-C164334
LifeSpan Biosciences
catalog: LS-C164334
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Cdk4 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The Cdk4 antibody detected the Cdk4 protein (arrow).
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CDK4 Antibody (clone IML-4) LS-C171093
LifeSpan Biosciences
catalog: LS-C171093
mouse monoclonal (IML-4)
reactivity: human
application: western blot, immunocytochemistry
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CDK4 Antibody (clone IML-4, Magnetic beads) LS-C171094
LifeSpan Biosciences
catalog: LS-C171094
mouse monoclonal (IML-4)
reactivity: human
conjugate: Bead
application: immunoprecipitation
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CDK4 Antibody (clone IML-4, Sepharose) LS-C171095
LifeSpan Biosciences
catalog: LS-C171095
mouse monoclonal (IML-4)
reactivity: human
application: immunoprecipitation
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CDK4 Antibody LS-C178280
LifeSpan Biosciences
catalog: LS-C178280
mouse monoclonal
reactivity: human
application: western blot

Western blot detection of CDK4 in Hela,LNCAP&F9 cell lysates using CDK4 antibody (1:1000 diluted).
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CDK4 Antibody (clone DCS-156) LS-C179590
LifeSpan Biosciences
catalog: LS-C179590
mouse monoclonal (DCS-156)
reactivity: human
application: western blot, immunohistochemistry
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CDK4 Antibody (aa211-304, clone 10B2179, Azide-free) LS-C183510
LifeSpan Biosciences
catalog: LS-C183510
mouse monoclonal (10B2179)
reactivity: human
application: western blot, ELISA, immunocytochemistry
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CDK4 Antibody (clone DCS-35) LS-C193354
LifeSpan Biosciences
catalog: LS-C193354
mouse monoclonal (DCS-35)
reactivity: human
application: western blot, immunoprecipitation
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CDK4 Antibody (clone DCS-156) LS-C193355
LifeSpan Biosciences
catalog: LS-C193355
mouse monoclonal (DCS-156)
reactivity: human
application: western blot, immunohistochemistry
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CDK4 Antibody (clone DCS-35) LS-C193409
LifeSpan Biosciences
catalog: LS-C193409
mouse monoclonal (DCS-35)
reactivity: human
application: western blot, immunoprecipitation
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CDK4 Antibody (clone DCS-156) LS-C193410
LifeSpan Biosciences
catalog: LS-C193410
mouse monoclonal (DCS-156)
reactivity: human
application: western blot, immunohistochemistry
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CDK4 Antibody (clone 4F11) LS-C196880
LifeSpan Biosciences
catalog: LS-C196880
mouse monoclonal (4F11)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western Blot analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11.Lane 1: CDK4 transfected lysate(33.7 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged CDK4 is approximately 1 ng/ml as a capture antibody.
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CDK4 Antibody (clone AT6D10) LS-C200855
LifeSpan Biosciences
catalog: LS-C200855
mouse monoclonal (AT6D10)
reactivity: human
application: western blot, ELISA

Western Blot: The cell lysate of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDK4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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CDK4 Antibody (aa219-246, AP) LS-C254432
LifeSpan Biosciences
catalog: LS-C254432
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: AP
application: western blot, ELISA
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to the supplier
CDK4 Antibody (aa219-246, Biotin) LS-C254433
LifeSpan Biosciences
catalog: LS-C254433
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA
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CDK4 Antibody (aa219-246, FITC) LS-C254434
LifeSpan Biosciences
catalog: LS-C254434
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA
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CDK4 Antibody (aa219-246, APC) LS-C254435
LifeSpan Biosciences
catalog: LS-C254435
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: APC
application: western blot, ELISA
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CDK4 Antibody (aa219-246, Azide-free, HRP) LS-C254436
LifeSpan Biosciences
catalog: LS-C254436
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA
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to the supplier
CDK4 Antibody (aa273-305, AP) LS-C254437
LifeSpan Biosciences
catalog: LS-C254437
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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price:
to the supplier
CDK4 Antibody (aa219-246, PE) LS-C254438
LifeSpan Biosciences
catalog: LS-C254438
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA
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to the supplier
CDK4 Antibody (aa273-305, APC) LS-C254439
LifeSpan Biosciences
catalog: LS-C254439
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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price:
to the supplier
CDK4 Antibody (aa273-305, Biotin) LS-C254440
LifeSpan Biosciences
catalog: LS-C254440
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity:
price:
to the supplier
CDK4 Antibody (aa273-305, FITC) LS-C254441
LifeSpan Biosciences
catalog: LS-C254441
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity:
price:
to the supplier
CDK4 Antibody (aa273-305, Azide-free, HRP) LS-C254446
LifeSpan Biosciences
catalog: LS-C254446
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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price:
to the supplier
CDK4 Antibody (aa273-305, PE) LS-C254447
LifeSpan Biosciences
catalog: LS-C254447
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, flow cytometry
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CDK4 Antibody LS-C285719
LifeSpan Biosciences
catalog: LS-C285719
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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to the supplier
CDK4 Antibody (aa253-303) LS-C290867
LifeSpan Biosciences
catalog: LS-C290867
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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CDK4 Antibody (aa6-295) LS-C293584
LifeSpan Biosciences
catalog: LS-C293584
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Knockout Varification: Lane 1: Wild-type Hela cell lysate; Lane 2: CDK4 knockout Hela cell lysate; Predicted MW: 33kDa ; Observed MW: 33kDa; Primary Ab: 1µg/ml Rabbit Anti-Human CDK4 Ab; Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;

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CDK4 Antibody (aa6-295) LS-C293590
LifeSpan Biosciences
catalog: LS-C293590
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant CDK4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CDK4 Antibody (aa6-295, Biotin) LS-C297298
LifeSpan Biosciences
catalog: LS-C297298
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot

Western blot of recombinant CDK4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CDK4 Antibody (aa6-295, FITC) LS-C303089
LifeSpan Biosciences
catalog: LS-C303089
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot

Western blot of recombinant CDK4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CDK4 Antibody LS-C306886
LifeSpan Biosciences
catalog: LS-C306886
mouse polyclonal
reactivity: human
application: western blot
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CDK4 Antibody LS-C309861
LifeSpan Biosciences
catalog: LS-C309861
domestic rabbit polyclonal
reactivity: human
application: western blot
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to the supplier
CDK4 Antibody (N-Terminus) LS-C312593
LifeSpan Biosciences
catalog: LS-C312593
domestic rabbit polyclonal
reactivity: human
application: western blot

WB of CDK4 antibody. Lane 1: Rat Thymus Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: A549 Cell Lysate. Lane 5: COLO320 Cell Lysate. Lane 6: JURKAT Cell Lysate.
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CDK4 Antibody (Biotin) LS-C316690
LifeSpan Biosciences
catalog: LS-C316690
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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CDK4 Antibody (FITC) LS-C316692
LifeSpan Biosciences
catalog: LS-C316692
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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CDK4 Antibody (HRP) LS-C316693
LifeSpan Biosciences
catalog: LS-C316693
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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to the supplier
CDK4 Antibody (aa219-246) LS-C320268
LifeSpan Biosciences
catalog: LS-C320268
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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to the supplier
CDK4 Antibody (aa273-305) LS-C320270
LifeSpan Biosciences
catalog: LS-C320270
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
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to the supplier
CDK4 Antibody LS-C330939
LifeSpan Biosciences
catalog: LS-C330939
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemistry of paraffin-embedded human esophageal cancer using CDK4 antibody at dilution of 1:100 (400x lens).

Western blot analysis of extracts of various cell lines, using CDK4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunofluorescence analysis of A-549 cells using CDK4 antibody. Blue: DAPI for nuclear staining.
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CDK4 Antibody (aa272-303) LS-C344589
LifeSpan Biosciences
catalog: LS-C344589
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry

Fluorescent image of MCF-7 cells stained with (Rat) Cdk4 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Rat Cdk4 Antibody western blot of rat lung tissue lysates (35 ug/lane). The rat Cdk4 antibody detected the rat Cdk4 protein (arrow).
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IHC-plus™ CDK4 Antibody (aa1-50) LS-B13047
LifeSpan Biosciences
catalog: LS-B13047
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)

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CDK4 Antibody (1-50 aa, N-terminal) LS-C386966
LifeSpan Biosciences
catalog: LS-C386966
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of Cdk4 antibody
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CDK4 Antibody (phospho-Thr172) LS-C411605
LifeSpan Biosciences
catalog: LS-C411605
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cell lines.
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