TNK2 / ACK1 Antibody (phospho-Tyr284) LS-C34825
LifeSpan Biosciences
catalog: LS-C34825
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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TNK2 / ACK1 Antibody (phospho-Tyr518) LS-C97107
LifeSpan Biosciences
catalog: LS-C97107
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated

Dot blot of anti-Phospho-ACK1-pY518 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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TNK2 / ACK1 Antibody (aa934-964) LS-C99164
LifeSpan Biosciences
catalog: LS-C99164
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

ACK1 Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The ACK1 antibody detected the ACK1 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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TNK2 / ACK1 Antibody (phospho-Tyr284) LS-C126531
LifeSpan Biosciences
catalog: LS-C126531
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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IHC-plus™ TNK2 / ACK1 Antibody (aa278-289) LS-B6360
LifeSpan Biosciences
catalog: LS-B6360
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-TNK2 / ACK1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.25 ug/ml.
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TNK2 / ACK1 Antibody (aa265-290) LS-C155930
LifeSpan Biosciences
catalog: LS-C155930
domestic rabbit polyclonal
reactivity: human
application: western blot

ACK1 Antibody (Center Y284) western blot of NCI-H460 cell line lysates (35 ug/lane). The ACK1 antibody detected the ACK1 protein (arrow).
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TNK2 / ACK1 Antibody (aa383-411) LS-C155931
LifeSpan Biosciences
catalog: LS-C155931
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).
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TNK2 / ACK1 Antibody (aa10-39) LS-C155932
LifeSpan Biosciences
catalog: LS-C155932
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).

Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with ACK1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of NCI-H292 cells using ACK1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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TNK2 / ACK1 Antibody (phospho-Tyr826) LS-C163579
LifeSpan Biosciences
catalog: LS-C163579
domestic rabbit polyclonal
reactivity: human

Dot blot of anti-Phospho-ACK1-pY826 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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TNK2 / ACK1 Antibody (phospho-Tyr284) LS-C198587
LifeSpan Biosciences
catalog: LS-C198587
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ACK1 (Phospho-Tyr284) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using ACK1 (Phospho-Tyr284) Antibody. The lane on the right is blocked with the phospho peptide.
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TNK2 / ACK1 Antibody (aa466-515) LS-C199759
LifeSpan Biosciences
catalog: LS-C199759
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from EOC20 cells, using ACK antibody.
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TNK2 / ACK1 Antibody (aa231-280) LS-C200077
LifeSpan Biosciences
catalog: LS-C200077
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from COLO205 cells using ACK1 antibody.
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TNK2 / ACK1 Antibody (aa265-290, AP) LS-C250669
LifeSpan Biosciences
catalog: LS-C250669
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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to the supplier
TNK2 / ACK1 Antibody (aa265-290, APC) LS-C250670
LifeSpan Biosciences
catalog: LS-C250670
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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to the supplier
TNK2 / ACK1 Antibody (aa265-290, Biotin) LS-C250671
LifeSpan Biosciences
catalog: LS-C250671
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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to the supplier
TNK2 / ACK1 Antibody (aa265-290, FITC) LS-C250672
LifeSpan Biosciences
catalog: LS-C250672
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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TNK2 / ACK1 Antibody (aa265-290, Azide-free, HRP) LS-C250673
LifeSpan Biosciences
catalog: LS-C250673
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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TNK2 / ACK1 Antibody (aa265-290, PE) LS-C250674
LifeSpan Biosciences
catalog: LS-C250674
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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price:
to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr826, AP) LS-C250675
LifeSpan Biosciences
catalog: LS-C250675
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA
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TNK2 / ACK1 Antibody (phospho-Tyr826, Biotin) LS-C250676
LifeSpan Biosciences
catalog: LS-C250676
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA
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price:
to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr826, APC) LS-C250677
LifeSpan Biosciences
catalog: LS-C250677
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA
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TNK2 / ACK1 Antibody (phospho-Tyr826, FITC) LS-C250678
LifeSpan Biosciences
catalog: LS-C250678
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA
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TNK2 / ACK1 Antibody (phospho-Tyr826, Azide-free, HRP) LS-C250680
LifeSpan Biosciences
catalog: LS-C250680
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA
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to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr826, PE) LS-C250681
LifeSpan Biosciences
catalog: LS-C250681
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA
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price:
to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr518, APC) LS-C274808
LifeSpan Biosciences
catalog: LS-C274808
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA
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price:
to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr518, AP) LS-C274809
LifeSpan Biosciences
catalog: LS-C274809
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA
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price:
to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr518, Biotin) LS-C274810
LifeSpan Biosciences
catalog: LS-C274810
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA
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price:
to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr518, FITC) LS-C274811
LifeSpan Biosciences
catalog: LS-C274811
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA
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price:
to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr518, Azide-free, HRP) LS-C274812
LifeSpan Biosciences
catalog: LS-C274812
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA
quantity:
price:
to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr518, PE) LS-C274813
LifeSpan Biosciences
catalog: LS-C274813
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA
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price:
to the supplier
TNK2 / ACK1 Antibody (aa934-964, AP) LS-C274814
LifeSpan Biosciences
catalog: LS-C274814
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
TNK2 / ACK1 Antibody (aa934-964, APC) LS-C274815
LifeSpan Biosciences
catalog: LS-C274815
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
TNK2 / ACK1 Antibody (aa934-964, Biotin) LS-C274816
LifeSpan Biosciences
catalog: LS-C274816
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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price:
to the supplier
TNK2 / ACK1 Antibody (aa934-964, FITC) LS-C274817
LifeSpan Biosciences
catalog: LS-C274817
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
TNK2 / ACK1 Antibody (aa934-964, Azide-free, HRP) LS-C274818
LifeSpan Biosciences
catalog: LS-C274818
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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price:
to the supplier
TNK2 / ACK1 Antibody (aa934-964, PE) LS-C274820
LifeSpan Biosciences
catalog: LS-C274820
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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to the supplier
TNK2 / ACK1 Antibody (aa250-299) LS-C291221
LifeSpan Biosciences
catalog: LS-C291221
domestic rabbit polyclonal
reactivity: human
application: western blot
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to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr518) LS-C291479
LifeSpan Biosciences
catalog: LS-C291479
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot analysis of lysates from HepG2 cells treated with EGF, using phospho-ACK (Phospho-Tyr518) antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ACK (Phospho-Tyr518) Antibody
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TNK2 / ACK1 Antibody (aa814-844) LS-C155934
LifeSpan Biosciences
catalog: LS-C155934
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
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to the supplier
TNK2 / ACK1 Antibody (aa265-290) LS-C319282
LifeSpan Biosciences
catalog: LS-C319282
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr826) LS-C319283
LifeSpan Biosciences
catalog: LS-C319283
domestic rabbit polyclonal
reactivity: human
application: ELISA
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to the supplier
TNK2 / ACK1 Antibody (phospho-Tyr518) LS-C326777
LifeSpan Biosciences
catalog: LS-C326777
domestic rabbit polyclonal
reactivity: human
application: ELISA
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price:
to the supplier
TNK2 / ACK1 Antibody (aa934-964) LS-C326779
LifeSpan Biosciences
catalog: LS-C326779
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
IHC-plus™ TNK2 / ACK1 Antibody LS-B12327
LifeSpan Biosciences
catalog: LS-B12327
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of extracts of various cell lines, using TNK2 antibody.

Immunofluorescence analysis of U2OS cell using TNK2 antibody. Blue: DAPI for nuclear staining.

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
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TNK2 / ACK1 Antibody (phospho-Tyr284) LS-C357153
LifeSpan Biosciences
catalog: LS-C357153
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using ACK1 (Phospho-Tyr284) antibody.

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody.

Immunofluorescence of A549 cells, using ACK1 (Phospho-Tyr284) antibody.
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TNK2 / ACK1 Antibody (phospho-Tyr284) LS-C358365
LifeSpan Biosciences
catalog: LS-C358365
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of ACK1 (pY284) expression in SHSY5Y (A); HeLa (B) whole cell lysates.

Immunohistochemical analysis of ACK1 (pY284) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of ACK1 (pY284) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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TNK2 / ACK1 Antibody (220-300 aa) LS-C381489
LifeSpan Biosciences
catalog: LS-C381489
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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TNK2 / ACK1 Antibody (220-300 aa) LS-C386793
LifeSpan Biosciences
catalog: LS-C386793
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of ACK antibody
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