IHC-plus™ TRIM28 / KAP1 Antibody (aa823-835) LS-B3928
LifeSpan Biosciences
catalog: LS-B3928
domestic goat polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

TRIM28 / KAP1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-TRIM28 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

TRIM28 / KAP1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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IHC-plus™ TRIM28 / KAP1 Antibody (clone 4E6) LS-B5439
LifeSpan Biosciences
catalog: LS-B5439
mouse monoclonal (4E6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody, clone 4E6.

Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody clone 4E6. GAPDH ( 36.1 kD ) used as control.
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TRIM28 / KAP1 Antibody (aa1-50) LS-C287177
LifeSpan Biosciences
catalog: LS-C287177
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 1
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TRIM28 / KAP1 Antibody (C-Terminus) LS-C31432
LifeSpan Biosciences
catalog: LS-C31432
domestic rabbit polyclonal
reactivity: human
application: western blot
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TRIM28 / KAP1 Antibody (clone 20A1) LS-C41046
LifeSpan Biosciences
catalog: LS-C41046
mouse monoclonal (20A1)
reactivity: human
application: western blot

HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TIF1beta antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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TRIM28 / KAP1 Antibody LS-C48687
LifeSpan Biosciences
catalog: LS-C48687
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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TRIM28 / KAP1 Antibody LS-C71214
LifeSpan Biosciences
catalog: LS-C71214
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry
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TRIM28 / KAP1 Antibody (N-Terminus) LS-C109592
LifeSpan Biosciences
catalog: LS-C109592
domestic rabbit polyclonal
reactivity: human
application: western blot
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TRIM28 / KAP1 Antibody (aa216-265) LS-C109698
LifeSpan Biosciences
catalog: LS-C109698
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ TRIM28 / KAP1 Antibody (clone 1D11) LS-B5440
LifeSpan Biosciences
catalog: LS-B5440
mouse monoclonal (1D11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

TRIM28 monoclonal antibody, clone 1D11. Western blot of TRIM28 expression in PC-12.

Anti-TRIM28 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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TRIM28 / KAP1 Antibody (C-Terminus) LS-C143979
LifeSpan Biosciences
catalog: LS-C143979
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry
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TRIM28 / KAP1 Antibody (aa178-207) LS-C160184
LifeSpan Biosciences
catalog: LS-C160184
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

TRIM28 Antibody western blot of K562 cell line lysates (35 ug/lane). The TRIM28 antibody detected the TRIM28 protein (arrow).

TRIM28 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Fluorescent confocal image of HeLa cell stained with TRIM28 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.
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TRIM28 / KAP1 Antibody (C-Terminus) LS-C178267
LifeSpan Biosciences
catalog: LS-C178267
mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot detection of TRIM28(C-terminus) in 293T&HepG2 cell lysates using TRIM28(C-terminus) antibody (1:1000 diluted).

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TRIM28 / KAP1 Antibody (clone TRI5I093) LS-C184240
LifeSpan Biosciences
catalog: LS-C184240
mouse monoclonal (TRI5I093)
reactivity: human
application: western blot, immunocytochemistry
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TRIM28 / KAP1 Antibody (clone AH9R7) LS-C190969
LifeSpan Biosciences
catalog: LS-C190969
mouse monoclonal (AH9R7)
reactivity: human
application: western blot
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TRIM28 / KAP1 Antibody (aa770-783) LS-C205034
LifeSpan Biosciences
catalog: LS-C205034
domestic goat polyclonal
reactivity: human
application: western blot

TRIM28 antibody (1 ug/ml) staining HeLa nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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TRIM28 / KAP1 Antibody LS-C211589
LifeSpan Biosciences
catalog: LS-C211589
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Transcription intermediary factor 1-beta antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 92 kDa. Observed band size: 92 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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TRIM28 / KAP1 Antibody (HRP) LS-C211590
LifeSpan Biosciences
catalog: LS-C211590
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Western blot of Transcription intermediary factor 1-beta antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 92 kDa. Observed band size: 92 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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TRIM28 / KAP1 Antibody (Biotin) LS-C211592
LifeSpan Biosciences
catalog: LS-C211592
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Western blot of Transcription intermediary factor 1-beta antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 92 kDa. Observed band size: 92 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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TRIM28 / KAP1 Antibody (aa178-207, AP) LS-C224441
LifeSpan Biosciences
catalog: LS-C224441
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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TRIM28 / KAP1 Antibody (aa178-207, APC) LS-C224442
LifeSpan Biosciences
catalog: LS-C224442
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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TRIM28 / KAP1 Antibody (aa178-207, Biotin) LS-C224443
LifeSpan Biosciences
catalog: LS-C224443
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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TRIM28 / KAP1 Antibody (aa178-207, FITC) LS-C224444
LifeSpan Biosciences
catalog: LS-C224444
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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TRIM28 / KAP1 Antibody (aa178-207, Azide-free, HRP) LS-C224446
LifeSpan Biosciences
catalog: LS-C224446
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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TRIM28 / KAP1 Antibody (aa178-207, PE) LS-C227139
LifeSpan Biosciences
catalog: LS-C227139
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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IHC-plus™ TRIM28 / KAP1 Antibody LS-B10061
LifeSpan Biosciences
catalog: LS-B10061
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of TRIM28 in human testis tissue lysate with TRIM28 antibody at 1 ug/ml.

Anti-TRIM28 / KAP-1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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TRIM28 / KAP1 Antibody (phospho-Ser824) LS-C286015
LifeSpan Biosciences
catalog: LS-C286015
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
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TRIM28 / KAP1 Antibody (phospho-Ser824) LS-C286347
LifeSpan Biosciences
catalog: LS-C286347
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunocytochemistry
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TRIM28 / KAP1 Antibody (aa650-700) LS-C287178
LifeSpan Biosciences
catalog: LS-C287178
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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TRIM28 / KAP1 Antibody (aa1-50) LS-C290489
LifeSpan Biosciences
catalog: LS-C290489
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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TRIM28 / KAP1 Antibody (aa550-600) LS-C290790
LifeSpan Biosciences
catalog: LS-C290790
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunoprecipitation
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TRIM28 / KAP1 Antibody (aa175-225) LS-C290791
LifeSpan Biosciences
catalog: LS-C290791
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunoprecipitation
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TRIM28 / KAP1 Antibody (phospho-Ser824) LS-C290793
LifeSpan Biosciences
catalog: LS-C290793
domestic rabbit polyclonal
reactivity: human, mouse
modification: phosphorylated
application: western blot
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TRIM28 / KAP1 Antibody LS-C308584
LifeSpan Biosciences
catalog: LS-C308584
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry
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TRIM28 / KAP1 Antibody (aa178-207) LS-C325941
LifeSpan Biosciences
catalog: LS-C325941
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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TRIM28 / KAP1 Antibody LS-C332012
LifeSpan Biosciences
catalog: LS-C332012
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of HeLa cells.
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