IHC-plus™ SAE1 Antibody LS-B420
LifeSpan Biosciences
catalog: LS-B420
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-SAE1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
quantity:
price:
to the supplier
IHC-plus™ SAE1 Antibody (clone 1G4-1G5) LS-B6203
LifeSpan Biosciences
catalog: LS-B6203
mouse monoclonal (1G4-1G5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of SAE1 expression in HeLa.

Immunofluorescence of monoclonal antibody to SAE1 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged SAE1 is approximately 0.1 ng/ml as a capture antibody.
quantity:
price:
to the supplier
SAE1 Antibody LS-C17948
LifeSpan Biosciences
catalog: LS-C17948
domestic sheep polyclonal
reactivity: human
application: western blot, immunocytochemistry
quantity:
price:
to the supplier
SAE1 Antibody (aa334-346) LS-C55427
LifeSpan Biosciences
catalog: LS-C55427
domestic goat polyclonal
reactivity: human
application: western blot

Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
quantity:
price:
to the supplier
SAE1 Antibody LS-C76152
LifeSpan Biosciences
catalog: LS-C76152
mouse monoclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
SAE1 Antibody (aa300-329) LS-C98475
LifeSpan Biosciences
catalog: LS-C98475
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

AOS1 Antibody (V315) western blot of 293 cell line lysates (35 ug/lane). The AOS1 antibody detected the AOS1 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
quantity:
price:
to the supplier
SAE1 Antibody LS-C108720
LifeSpan Biosciences
catalog: LS-C108720
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of SAE1 in SK-N-SH lysate with SAE1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
quantity:
price:
to the supplier
SAE1 Antibody (N-Terminus) LS-C111368
LifeSpan Biosciences
catalog: LS-C111368
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
SAE1 Antibody (aa71-120) LS-C111369
LifeSpan Biosciences
catalog: LS-C111369
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
SAE1 Antibody (aa221-270) LS-C118599
LifeSpan Biosciences
catalog: LS-C118599
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HUVEC cells, using AOS1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using AOS1 Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier
SAE1 Antibody LS-C132310
LifeSpan Biosciences
catalog: LS-C132310
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier
SAE1 Antibody LS-C153857
LifeSpan Biosciences
catalog: LS-C153857
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier
SAE1 Antibody (phospho-Ser185) LS-C154247
LifeSpan Biosciences
catalog: LS-C154247
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
quantity:
price:
to the supplier
SAE1 Antibody (clone 2946C4a) LS-C184674
LifeSpan Biosciences
catalog: LS-C184674
mouse monoclonal (2946C4a)
reactivity: human
application: western blot, immunocytochemistry
quantity:
price:
to the supplier
SAE1 Antibody (aa3-247) LS-C186319
LifeSpan Biosciences
catalog: LS-C186319
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: A431 , B: H1299. 10% SDS PAGE. SAE1 antibody diluted at 1:1000.

Immunofluorescence of paraformaldehyde-fixed HeLa using SAE1 antibody at 1:200 dilution.

IHC of paraffin-embedded A549 Xenograft using SAE1 antibody at 1:100 dilution.
quantity:
price:
to the supplier
IHC-plus™ SAE1 Antibody (clone 1G8) LS-B9408
LifeSpan Biosciences
catalog: LS-B9408
mouse monoclonal (1G8)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1.

Anti-SAE1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAE1.

Anti-SAE1 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
quantity:
price:
to the supplier
SAE1 Antibody (aa300-329, AP) LS-C206905
LifeSpan Biosciences
catalog: LS-C206905
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SAE1 Antibody (aa300-329, APC) LS-C206906
LifeSpan Biosciences
catalog: LS-C206906
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SAE1 Antibody (aa300-329, Biotin) LS-C206907
LifeSpan Biosciences
catalog: LS-C206907
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SAE1 Antibody (aa300-329, FITC) LS-C206908
LifeSpan Biosciences
catalog: LS-C206908
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SAE1 Antibody (aa300-329, PE) LS-C206912
LifeSpan Biosciences
catalog: LS-C206912
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SAE1 Antibody (aa300-329, Azide-free, HRP) LS-C209157
LifeSpan Biosciences
catalog: LS-C209157
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SAE1 Antibody (aa296-346) LS-C289632
LifeSpan Biosciences
catalog: LS-C289632
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunoprecipitation
quantity:
price:
to the supplier
SAE1 Antibody (aa300-329) LS-C328802
LifeSpan Biosciences
catalog: LS-C328802
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SAE1 Antibody (clone OTI6C7) LS-C339476
LifeSpan Biosciences
catalog: LS-C339476
mouse monoclonal (OTI6C7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1.

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
SAE1 Antibody (clone OTI6B11) LS-C339477
LifeSpan Biosciences
catalog: LS-C339477
mouse monoclonal (OTI6B11)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1.

IHC of paraffin-embedded Human breast tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
SAE1 Antibody (clone OTI3C11) LS-C339478
LifeSpan Biosciences
catalog: LS-C339478
mouse monoclonal (OTI3C11)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1.
quantity:
price:
to the supplier
SAE1 Antibody (clone OTI3C3) LS-C339550
LifeSpan Biosciences
catalog: LS-C339550
mouse monoclonal (OTI3C3)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1.

IHC of paraffin-embedded Human breast tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
SAE1 Antibody (clone OTI2B2) LS-C339570
LifeSpan Biosciences
catalog: LS-C339570
mouse monoclonal (OTI2B2)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1.
quantity:
price:
to the supplier
SAE1 Antibody (Internal) LS-C353229
LifeSpan Biosciences
catalog: LS-C353229
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of SAE1 expression in Jurkat (A); A431 (B); HeLa (C) whole cell lysates.

Immunohistochemical analysis of SAE1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of SAE1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
SAE1 Antibody (190-270 aa, Internal) LS-C385989
LifeSpan Biosciences
catalog: LS-C385989
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of SAE1 antibody
quantity:
price:
to the supplier