IHC-plus™ SAE2 / UBA2 Antibody (aa160-210) LS-B2091
LifeSpan Biosciences
catalog: LS-B2091

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-UBA2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ SAE2 / UBA2 Antibody LS-B5092
LifeSpan Biosciences
catalog: LS-B5092

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of SAE2 in 293 cell lysate with SAE2 antibody at 0.25 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Anti-UBA2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of SAE2 in 293 cells with SAE2 antibody at 4 ug/ml.
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SAE2 / UBA2 Antibody (aa2-14) LS-C55428
LifeSpan Biosciences
catalog: LS-C55428

domestic goat polyclonal
reactivity: human
application: western blot, immunocytochemistry

SAE2 / UBA2 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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SAE2 / UBA2 Antibody (aa601-631) LS-C96603
LifeSpan Biosciences
catalog: LS-C96603

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

The anti-UBA2 C-term E616 Antibody is used in Western blot to detect UBA2 in Jurkat lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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IHC-plus™ SAE2 / UBA2 Antibody (aa591-640) LS-B7654
LifeSpan Biosciences
catalog: LS-B7654

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-UBA2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot analysis of lysates from 293 cells, treated with UV 5', using Uba2 Antibody. The lane on the right is blocked with the synthesized peptide.
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SAE2 / UBA2 Antibody (Ser621) LS-C176362
LifeSpan Biosciences
catalog: LS-C176362

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Uba2 (S621) pAb in extracts from 293 cells treated with UV 5'.

IHC of Uba2 (S621) pAb in paraffin-embedded human lung carcinoma tissue.
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IHC-plus™ SAE2 / UBA2 Antibody (clone 4A3) LS-B9990
LifeSpan Biosciences
catalog: LS-B9990

mouse monoclonal (4A3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-UBA2 monoclonal antibody.

Anti-UBA2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBA2.

Anti-SAE2 / UBA2 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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SAE2 / UBA2 Antibody (aa601-631, AP) LS-C246056
LifeSpan Biosciences
catalog: LS-C246056

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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SAE2 / UBA2 Antibody (aa601-631, APC) LS-C246057
LifeSpan Biosciences
catalog: LS-C246057

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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SAE2 / UBA2 Antibody (aa601-631, Biotin) LS-C246058
LifeSpan Biosciences
catalog: LS-C246058

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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SAE2 / UBA2 Antibody (aa601-631, FITC) LS-C246059
LifeSpan Biosciences
catalog: LS-C246059

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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SAE2 / UBA2 Antibody (aa601-631, PE) LS-C246064
LifeSpan Biosciences
catalog: LS-C246064

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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SAE2 / UBA2 Antibody (aa601-631, Azide-free, HRP) LS-C247907
LifeSpan Biosciences
catalog: LS-C247907

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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SAE2 / UBA2 Antibody (aa525-575) LS-C289633
LifeSpan Biosciences
catalog: LS-C289633

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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SAE2 / UBA2 Antibody (aa590-640) LS-C289634
LifeSpan Biosciences
catalog: LS-C289634

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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SAE2 / UBA2 Antibody (aa601-631) LS-C328801
LifeSpan Biosciences
catalog: LS-C328801

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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SAE2 / UBA2 Antibody (C-Terminus) LS-C353219
LifeSpan Biosciences
catalog: LS-C353219

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of UBA2 expression in A549 (A); Caco2 (B); HeLa (C); HepG2 (D) whole cell lysates.

Immunohistochemical analysis of UBA2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of UBA2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SAE2 / UBA2 Antibody (560-640 aa, C-terminal) LS-C386557
LifeSpan Biosciences
catalog: LS-C386557

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of UBA2 antibody
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