GENLISA™ Mouse IL-12 ELISA | Krishgen Biosystems KLM0036 product information

product summary

company name :

Krishgen Biosystems

product type :

ELISA/assay

product name :

GENLISA™ Mouse IL-12 ELISA

catalog :

KLM0036

quantity :

1 x 96 wells

price :

270 USD

more info or order :

Krishgen Biosystems product webpage

citations: 3

Reference
Ingawale D, Mandlik S, Patel S. Combination of Sarsasapogenin and Fluticasone attenuates ovalbumin-induced airway inflammation in a mouse asthma model. Immunopharmacol Immunotoxicol. 2020;42:128-137 pubmed publisher
Ingawale D, Mandlik S, Patel S. Anti-inflammatory potential of hecogenin on atopic dermatitis and airway hyper-responsiveness by regulation of pro-inflammatory cytokines. Immunopharmacol Immunotoxicol. 2019;41:327-336 pubmed publisher
Ingawale D, Patel S. Hecogenin exhibits anti-arthritic activity in rats through suppression of pro-inflammatory cytokines in Complete Freund's adjuvant-induced arthritis. Immunopharmacol Immunotoxicol. 2017;:1-13 pubmed publisher

product information

Catalog Number :

KLM0036

Product Name :

GENLISA™ Mouse IL-12 ELISA

Product Type :

ELISA

Size :

1 x 96 wells

List Price (USD) :

270 USD

Product Description :

This ELISA is intended for the quantitative detection of IL-12 in mouse serum, cell cuture supernatant and plasma samples. ELISA Features include:- Ready to use protocol, Standardisation and High Reproducibility, Lot to Lot Consistency, Accuracy and Precision. Validated against seven points for a Gold Ring Standard Quality ELISA - the benchmark sign for Krishgen quality, Long shelf-life, Expert technical support. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, biological fluids and cell culture supernatant. The kit employs a double-antibody sandwich ELISA technique which leads to higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.

SpeciesSummary :

Mouse

Background :

The Genlisa™ ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum / plasma as validated with the kit. The kit employs a sandwhich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibodies. Double antibodies are used in this kit.

Packaging :

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

Storage :

2-8 Deg C

Calibration Range :

30 pg/ml – 480 ng/ml

Sensitivity :

20 pg/ml

Assay Principle :

The method employs a sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and analyte present in the sample are bound by the antibodies. Biotin labeled anti-analyte antibodies are added and followed by Streptavidin-HRP pipetting and incubation to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of analyte in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Biomarker :

IL-12

Regulatory Status :

RUO

Shipping :

2-8 degrees C

more info or order :

Krishgen Biosystems product webpage