ELISA/assay

GENLISA™ Mouse SARS-CoV-2 IgG Antibody to Spike RBD protein ELISA (Quantitative)

KBVH015-13

1 x 96 wells

485 USD

GENLISA™ Mouse SARS-CoV-2 IgG Antibody to Spike RBD protein ELISA (Quantitative)

ELISA

1 x 96 wells

485 USD

ELISA for the quantitative determination of IgG Antibodies to SARS-CoV-2 Spike Protein in mouse serum and plasma. Accurate, specific detection with reproducible results.

Mouse

The GENLISA™ ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

0 ng/ml - 720 ng/ml

12 ng/ml

The method employs indirect sandwich ELISA technique. SARS-CoV-2 Spike RBD-His Recombinant protein is pre-coated onto microwells. Samples and standards are pipetted into microwells and Antibodies to Mouse Anti-SARS-CoV-2 (2019-nCoV) present in the sample are bound by the protein antigen. After incubation the wells are washed and followed by addition of HRP-conjugated Detection IgG Antibody into each well and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of AntiMouse Anti-SARS-CoV-2 (2019-nCoV) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

SARS-CoV-2 IgG Antibody to Spike RBD protein

RUO

2-8 degrees C