ELISA/assay

KRIBIOLISA™ Romiplostim (NPLATE) ELISA

KBI1602

1 x 96 wells

1050 USD

KRIBIOLISA™ Romiplostim (NPLATE) ELISA

ELISA

1 x 96 wells

1050 USD

Romiplostim ELISA for the quantative determination of Romiplostim in human serum and plasma samples.

Human

Romiplostim (NPlate™) is a fusion protein analog of thrombopoietin, a hormone that regulates platelet production. Romiplostim is an Fc-peptide fusion protein (peptibody) that signals and activates intracellular transcriptional pathways via the TPO receptor (also known as cMpl) to increase platelet production. The peptibody molecule is comprised of a human immunoglobulin IgG1 Fc domain, with each single-chain subunit covalently linked at the C-terminus to a peptide chain containing 2 TPO receptor-binding domains. Romiplostim has no amino acid sequence homology to endogenous TPO. In pre-clinical and clinical trials no anti-romiplostim antibodies cross reacted with endogenous TPO. Romiplostim is indicated for adult chronic immune (idiopathic) thrombocytopenic purpura (ITP) patients who are refractory to other treatments (e.g. corticosteroids, immunoglobulins).

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

0 ng/ml - 640 ng/ml

Inquire

Romiplostim ELISA is a two-step enzyme immunoassay on the basis of humanized monoclonal antibodies specific to c-Mpl. In the first step, Assay Diluent and Standard or Samples are dispensed simultaneously into the wells of a microtitration plate coated with humanized monoclonal antibodies specific to c-Mpl. In the second step, horseradish peroxidase (HRP) labelled humanized monoclonal antibodies specific to -Mpl are dispensed in respective wells. After incubation unbound components are removed by a washing step. HRP converts the subsequently added substrate solution of 3,3’,5,5’- Tetramethylbenzidine (TMB) within a 30 min reaction time into a blue product. The enzyme reaction is terminated by stop solution dispensed into the wells turning the solution from blue to yellow. The optical density (OD) of the solution read at 450 is directly proportional to the specifically bound amount of Romiplostim.

Romiplostim

RUO

2-8 degrees C