ELISA/assay

KRIBIOLISA™ Necitumumab (PORTRAZZA) ELISA

KBI1496

1 x 96 wells

1050 USD

KRIBIOLISA™ Necitumumab (PORTRAZZA) ELISA

ELISA

1 x 96 wells

1050 USD

Necitumumab (PORTRAZZA) ELISA for quantitative determination of Necitumumab (PORTRAZZA) in human serum, plasma Krishgen PK kits, developed using the innovator drug as calibrator, are used for the quantitation of serum protein drug levels, and can be used for biosimilar and generics research as well. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year

Human

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

0 ng/ml - 640 ng/ml

7.5 ng/ml

This ELISA employs the quantitative sandwich enzyme immunoassay technique. Antibodies to the mAb are pre-coated onto microwells. Samples and standards are pipetted into microwells and the target mAb drug present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-drug antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mAb drug in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Necitumumab

RUO

2-8 degrees C