ELISA/assay

KRIBIOLISA™ Panitumumab (VECTIBIX) ELISA

KBI1082

1 x 96 wells

940 USD

KRIBIOLISA™ Panitumumab (VECTIBIX) ELISA

ELISA

1 x 96 wells

940 USD

Sensitivity: 80ng/ml Sample Type: Serum, Plasma Calibrator Range: 0 -1000 ng/ml Enzyme Immunoassay for quantitative detection of Panitumumab in serum and plasma. Panitumumab, is a IgG2 human monoclonal antibody specific to the epidermal growth factor receptor. EGFR is a transmembrane protein. Panitumumab works by binding to the extracellular domain of the EGFR preventing its activation. This results in halting of the cascade of intracellular signals dependent on this receptor. Krishgen PK kits, developed using the innovator drug as calibrator, are used for the quantitation of serum protein drug levels, and can be used for biosimilar and generics research as well. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The method employs the quantitative enzyme immunoassay technique. Standards or samples are pipetted into microwells pre-coated with Anti- Panitumumab antibody and Panitumumab present in the sample and standards are bound by Anti- Panitumumab antibody. In the second step, Detection antidody is added and incubated. In the third step, a HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing. Addition of TMB substrate will develop blue color and intensity of blue colour in wells is proportional to the concentration of Panitumumab present in standard or sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Human

Panitumumab, is a IgG2 human monoclonal antibody specific to the epidermal growth factor receptor. EGFR is a transmembrane protein. Panitumumab works by binding to the extracellular domain of the EGFR preventing its activation. This results in halting of the cascade of intracellular signals dependent on this receptor.

This ELISA kit comes with a pre-coated plate, concentrated or lyophilized standards, wash solution, subtrate, stop solution and all the required diluents. For the exact contents of the kit, please refer to the datasheet / IFU.

2-8 Deg C

50 ng/ml - 1000 ng/ml

2.5 ng/ml

The method employs the quantitative enzyme immunoassay technique. Standards or samples are pipetted into microwells pre-coated with Anti- Panitumumab antibody and Panitumumab present in the sample and standards are bound by Anti- Panitumumab antibody. In the second step, Detection antidody is added and incubated. In the third step, a HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing. Addition of TMB substrate will develop blue color and intensity of blue colour in wells is proportional to the concentration of Panitumumab present in standard or sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Panitumumab

RUO

2-8 degrees C